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The present study was conducted in the month of August, 2011 to determine the seroprevalence of brucellosis in organized herd of buffaloes with a history of abortions located in North Gujarat. For the purpose, a total of 117 serum samples were collected from buffaloes and were screened for the presence of antibodies against Brucella by Rose Bengal plate test. The overall sero-prevalence recorded as 25.64%. Age-wise prevalence indicated that 30.53% animals reacted positive to the test was of above 60 months age. However, in less than 60 months of age groups, only 4.55% animals were positive. Prevalence of brucellosis in aborted animals was 72.00% as compared to 13.04% in animals without history of abortion. The study indicated high-seropositivity of brucellosis in the farm and to prevent economic losses to the farm, it is necessary to develop and adopt various control measures.