Comparative studies of certain enzyme assay during various reproductive states in buffaloes

Abstract

Thirty healthy Murrah buffaloes of 5-9 years of age with second to fifth lactation were randomly chosen from well organized dairies located at Jabalpur to elucidate alteration in concentration of some enzymes viz. serum alkaline phosphatase (ALP), acid phosphatase (ACP), alanine transferase (ALT), and aspartate amino transferase (AST), in serum of normal cyclic, repeat breeder and anoestrus Murrah buffaloes. Gynecological examinations were employed for the diagnosis of reproductive status of animals. The selected 30 Murrah buffaloes were divided into three groups and each group comprised of 10 animals for the generation of experimental data. Blood samples were collected from animals of different groups on the day of estrus from normal cyclic, repeat breeder and anoestrus buffaloes on the same day for gynaeco-clinical examination. The serum was prepared following routine procedure. The serum enzymatic activities was monitored immediately for serum alkaline and acid phophatase, alanine amino transferase and aspartate amino transferase. The serum alkaline phosphatase and acid phosphatase values were 9.190 and 1.357 ; 19.778 and 2.667; 14.064 and 2.100 KA units /100 ml in anoestrus , repeat breeder and normal cyclic groups, respectively. The serum alkaline phosphatase and acid phosphatases values were significantly (P<0.01) different among the groups. It was recorded to be highest in repeat breeder followed by normal cyclic and lowest in anestrus buffaloes. The serum alanine aminotransferase and aspartate aminotransferase values were 26.402 and 69.124; 32.035 and 104.435; 34.007 and 85.093 U/L in anoestrus , repeat breeder and normal cyclic groups, respectively. The values of serum alanine aminotransferase and aspartate aminotransferase of repeat breeder was significantly (P<0.01) higher than normal cyclic and lowest in anoestrus group.