In silico characterization of Mx2 protein of buffalo (Bubalus bubalis)

Authors

  • L. Buragohain Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Assam, India
  • P. Borah Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Assam, India
  • N. Goswami Bioinformatics Infrastructure Facility, College of Veterinary Science, Assam Agricultural University, Assam, India
  • R. Dutta Advanced State Biotech Hub (Assam), College of Veterinary Science, Assam Agricultural University, Assam, India

Keywords:

Bubalus bubalis, buffalo, Mx2 protein, 3D structure

Abstract

The Myxovirus resistance protein 2 of Homo sapiens is well characterized in terms of structure and function but very little is known about myxovirus resistance protein 2 (Mx2) of Bubalus bubalis. Although a few functions of buffalo Mx2 protein are known, its structural characteristics have still remained unclear. In the present study, buffalo Mx2 protein was characterized by defining its possible structure and functions using several online and offline computational tools. Besides the physico-chemical characteristics, various post translational modifcations, localization, possible domains and interactions with other probable proteins were predicted using its amino acid sequence. In addition to the secondary structure, the three-dimensional structure was predicted using I-TASSER homology modelling server and a refined model was constructed in Modeller. The refined model manifested acceptable quality and stereochemistry on validation with different programs. Furthermore, a phylogenetic analysis of the protein was done and Mx2 protein of buffalo was found to have a common ancestry with that of other bovidae family members. These curated observations might provide a sound foundation for further structural and functional characterization of buffalo Mx2 protein.

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Author Biographies

L. Buragohain, Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Assam, India

Lukumoni Buragohain

P. Borah, Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Assam, India

Probodh Borah

N. Goswami, Bioinformatics Infrastructure Facility, College of Veterinary Science, Assam Agricultural University, Assam, India

Nabajyoti Goswami

R. Dutta, Advanced State Biotech Hub (Assam), College of Veterinary Science, Assam Agricultural University, Assam, India

Rupam Dutta

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Published

2019-12-27

How to Cite

Buragohain, L., Borah, P., Goswami, N., & Dutta, R. (2019). In silico characterization of Mx2 protein of buffalo (Bubalus bubalis). Buffalo Bulletin, 38(4), 629–647. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/1608

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Original Article