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The present research work was carried out to evaluate efficacy luteotropic hormones and its effect on progesterone concentration in non-infectious repeat breeding Murrah buffaloes. The research was conducted at Department of Animal Reproduction, Gynaecology and Obstetrics and Radio Immunay Assay (RIA) Laboratory of Bombay Veterinary College, Mumbai and buffalo dairy farms of Aarey Colony, Goregaon, Mumbai. Out of 100 repeat breeding Murrah buffaloes of 6 to 8 years old, thirty two pluriparous Murrah buffaloes of non-infectious origin were selected on the basis of white side test for the present study. The Murrah buffaloes were randomly devided into four groups with eight animals in each group. The Group I was treated with Inj. GnRH 20 μg while Group II and Group III were treated with Inj. hCG 1500 IU and 3000 IU, respectively on day 7 after artificial insemination. Group IV was kept as control. The pregnancy was confirmed after 2 months of artificial insemination by per rectal examination. In present research work, the conception rates in Murrah buffaloes of Group I, II, and III were 50.00% (4/8), 50.00% (4/8) and 62.50% (5/8), respectively. In Group IV none of the eight Murrah buffaloes was conceived. The overall conception rate in the present study was 40.67% (13/32). The mean serum progesterone concentration in Group I, II, III and IV on day 7 was 2.53±0.26, 2.57±0.39, 3.77±0.58, 2.54±0.28 ng/ml, respectively while on day 14 it was 5.74±0.19, 5.99±1.06, 7.34±0.90, 3.96±0.27 ng/ml, respectively. There was no significant difference in mean progesterone concentration between Group I, II, III and IV on day 7 but there was significant difference in mean serum progesterone concentration between Group I, II, III and IV at 5% level on day 14. All the groups treated with respective protocols showed significant increase in the mean serum progesterone concentration on day 14 from day 7. This significant increase clearly indicates that the hormones GnRH 20 μg, hCG 1500 IU and hCG 3000 IU can be effectively used to enhance the progesterone level in non-infectious repeat breeder Murrah buffaloes when given on day 7 after artificial insemination.