Recovery trends and correlation of non invasively isolated mammary epithelial cells with milk yield in Surti and Mehsani buffaloes during early lactation

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Kuldeep Kumar Tyagi Balkrushna Pratapchand Brahmkshtri Vishnubhai Bhikabhai Kharadi Umed Vitthalbhai Ramani Gaurav Mulvant Pandya Mamta Janmeda


This study aims to elucidate recovery trends and correlation of non-invasively isolated mammary epithelial cells with milk yield in Surti and Mehsani buffalo breeds during early lactation. Surti and Mehsani buffaloes, 13 each maintained at Livestock Research Station, Navsari and Sardarkrushinagar, Dantiwada, Gujarat, India respectively were selected for the study. Milk samples were collected repeatedly from the same buffaloes at 15th and 60th day postpartum (pp). Buffaloes were categorized for data analysis and comparisons in to four groups viz. S15 (Surti buffaloes 15th day pp), S60 (Surti buffaloes 60th day pp), M15 (Mehsani buffaloes 15th day pp) and M60 (Mehsani buffaloes 60th day pp). The total somatic cells were separated from milk samples through centrifugation and primary bovine mammary epithelial cells (pBMEC) were obtained from total somatic cells using antibody mediated magnetic separation method. There was significant decrease in mean somatic cell count (SCC) with advancement of lactation to the tune of 18.72% in Surti and 16.84%  in Mehsani buffaloes from day 15 to 60 pp. Comparable recovery of pBMEC (2.13 to 3.45%) from total somatic cells was observed over the advancement of lactation and between breeds. The pBMEC and SCC had exhibited negligible to low and non significant correlations with cumulative milk yield (CMY) and Test day milk yield (TDMY). Thus, it can be concluded that pBMEC can successfully be recovered from somatic cells. pBMEC and SCC both does not proved to be a significant explanatory variable for milk yield in buffalo breeds under study.


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TYAGI, Kuldeep Kumar et al. Recovery trends and correlation of non invasively isolated mammary epithelial cells with milk yield in Surti and Mehsani buffaloes during early lactation. Buffalo Bulletin, [S.l.], v. 40, n. 2, p. 317-323, june 2021. ISSN 2539-5696. Available at: <>. Date accessed: 28 sep. 2022.
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Alcorn, J., X. Lu, J.A. Moscow and P.J. McNamara. 2002. Transporter gene expression in lactating and nonlactating human mammary epithelial cells using real-time reverse transcription-polymerase chain reaction. J. Pharmacol. Exp. Ther., 303(2): 487-496. DOI: 10.1124/jpet.102.038315
Boutinaud, M., M.H. Ben Chedly, E. Delamaire and J. Guinard-Flament. 2008. Milking and feed restriction regulate transcripts of mammary epithelial cells purified from milk. J. Dairy Sci., 91(3): 988-998. DOI: 10.3168/jds.2007-0587.
Boutinaud, M. and H. Jammes. 2002. Potential uses of milk epithelial cells: A review. Reprod. Nutr. Dev., 42(2): 133-147. DOI: 10.1051/rnd:2002013
Boutinaud, M., V. Lollivier, L. Finot, R.M. Bruckmaier and P. Lacasse. 2012. Mammary cell activity and turnover in dairy cows treated with the prolactin-release inhibitor quinagolide and milked once daily. J. Dairy Sci., 95(1): 177-187. DOI: 10.3168/jds.2011-4461
Boutinaud, M., H. Rulquin, D.H. Keisler, J. Djiane and H. Jammes. 2002. Use of somatic cells from goat milk for dynamic studies of gene expression in the mammary gland. J. Anim. Sci., 80(5): 1258-1269. DOI: 10.2527/2002.8051258x
Buehring, G.C. 1990. Culture of mammary epithelial cells from bovine milk. J. Dairy Sci., 73(4): 956-963. DOI: 10.3168/jds.S0022-0302(90)78752-8.
Ceron-Munoz, M., H. Tonhati, J. Duarte, J. Oliveira, M. Munoz-Berrocal and H. Jurado-Gamez. 2002. Factors affecting somatic cell counts and their relations with milk and milk constituent yield in buffaloes. J Dairy Sci., 85: 2885-2889. DOI: 10.3168/jds.S0022-0302(02)74376-2.
Dhakal, I.P. 2006. Normal somatic cell count and subclinical mastitis in Murrah buffaloes. Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health, 53: 81-86. DOI: 10.1111/j.1439-0450.2006.00918.x.
Feng, S., A.M. Salter, T. Parr and P.C. Garnsworthy. 2007. Extraction and quantitative analysis of stearoyl-coenzyme A desaturase mRNA from dairy cow milk somatic cells. J. Dairy Sci., 90(9): 4128-4136. DOI: 10.3168/jds.2006-830.
Gomm, J.J., P.J. Browne, R.C. Coope, Q.Y. Liu, L. Buluwela and R.C. Coombes. 1995. Isolation of pure populations of epithelial and myoepithelial cells from the normal human mammary gland using immunomagnetic separation with Dynabeads. Analitycal Biochemistry, 226(1): 91-99. DOI: 10.1006/abio.1995.1196.
Kavitha, K.L., K. Rajesh, K. Suresh, K. Satheesh and N.S. Sunder. 2009. Buffalo mastitis-Risk factors. Buffalo Bull., 28(3): 135-137. Available on:
Miller, R.H., M.J. Paape and L.A. Fulton. 1991. Variation in milk somatic cells of heifers at first calving. J. Dairy Sci., 74: 3782-3790. DOI: 10.3168/jds.S0022-0302(91)78570-6.
Muggli, J. 1995. Influence of stage of lactation on somatic cell counts. Animal Breeding Abstracts, 1996.
Nandi, S. 2010. Enzymatic alteration in buffalo milk related to udder health status. M.V.Sc. Thesis, Maharashtra Animal and Fishery Sciences, Nagpur, Maharashtra, India.
Patil, M.P., A.S. Nagvekar, S.D. Ingole, S.V. Bharucha and V.T. Palve. 2015. Somatic cell count and alkaline phosphatase activity in milk for evaluation of mastitis in buffalo. Vet. World, 8(3): 8-11. DOI: 10.14202/vetworld.2015.363-366.
Rodriguez-Zas, S.L., D. Gianola and G.E. Shook. 2000. Evaluation of models for somatic cell score lactation patterns in Holsteins. Livest. Prod. Sci., 67: 19-30. DOI: 10.1016/S0301-6226(00)00193-7.
Sarubbi, F., F. Polimeno, G. Auriemma, G. Maglione, R. Baculo and R. Palomba. 2013. Effects of season calving and managements on lactating curves in two different farms (organic vs conventional) in buffalo cows. Open Journal of Animal Sciences, 3(1): 83-87.
Sharma, N., N.K. Singh and M.S. Bhadwal. 2011. Relationship of somatic cell count and mastitis: An overview. Asian Austral. J. Anim. Sci., 24(3): 429-438. DOI: 10.5713/ajas.2011.10233.
Shook, G.E. and M.M. Schutz. 1994. Selection on somatic cell score to improve resistance to mastitis in the United States. J. Dairy Sci., 77(2): 648-658. DOI: 10.3168/jds.S0022-0302(94)76995-2.
Sigl, T., H.H.D. Meyer and S. Wiedemann. 2012. Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation. Czech J. Anim. Sci., 57(10): 469-480. Available on: https://www.agriculturejournals.c
Silva, I.D. and K.F.S.T. Silva. 1994. Total and differential cell counts in buffalo milk. Buffalo J., 2: 133-137.