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The present study was conducted in buffaloes to determine the occurrence of subclinical mastitis and its diagnosis in relation to milk SCC and biochemical composition. The results showed that taking quarter foremilk (QFM) somatic cell count (SCC) threshold limit of<200×103 cell for defining healthy quarters, 16.48% of buffaloes and 5.79% of quarters were positive for specific subclinical mastitis. In total, 57/363 (15.70%)of quarters were bacteriological positive with 21 (5.79%) representing specific and 36 (9.92%) latent infections. The major pathogen isolated were 28(49%)coagulase-negative staphylococci followed by 16(28%) S. aureus, 09 (16%) Streptococcus and 4(7%) Corynebacterium spp. The average QFM SCC was found as 52.50×103 cells /ml for the quarter with no bacteria, and 356.51×103 cell /ml for the quarters infected with one or the other bacteria (p<0.05). The milk electrical conductivity (EC), Fat and Lactoseshowed significant alterations with the quarter infection; the EC and Fat increased while Lactose decreased in infected quarters (p < 0.05). The California mastitis test CMT) at cut off score of > 0.5 for diseased quarters showed 68.75% sensitivity with 99.09% specificity. The EC with 87.50% sensitivity and 74.02% specificity and Lactose with 84.37% sensitivity and 66.16% specificity also showed significant (p<0.01) discrimination ability to differentiate healthy and mastitis quarters in buffaloes. The milk pH was not found to be a good parameter to identify diseased quarters.
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