Therapeutic management of subclinical mastitis in buffaloes
Keywords:
Bubalus bubalis, buffaloes, marbofloxacin, vitamin E, selenium, trisodium citrate, subclinical mastitisAbstract
The present investigation was carried out to study the therapeutic efficacy of marbofloxacin along with vitamin E, selenium and trisodium citrate against sublinical mastitis in buffaloes. The study was carried out on lactating buffaloes maintained at different organized dairy farms in and around Bidar, Karnataka. Screening of subclinical mastitis was done by California mastitis test (CMT) and Electrical Conductivity (EC). Milk samples positive for subclinical mastitis were considered for isolation and identification of bactria pathogens by cultural examination and biochemical tests. 18 out of 81 lactating buffaloes were found positive for subclinical mastitis affecting one or two quarters giving a quarter wise prevalence of 11.33% and animal wise prevalence of 22.22%. Out of 27 positive milk samples 14 (51.85%) were found positive for Staphylococcus spp. followed by Streptococcus spp. (33.33%) and E. coli (14.81%). The antibiogram of the bacterial isolates to standard antibiotic discs determined by disc diffusion method revealed highest sensitivity to gentamicin and enrofloxacin followed by cefteriaxone, moxifloxacin, cefoperazone, tetracycline and least sensitive to amoxicillin with clavalanic acid. Positive quarters treated with single dose of marbofloxacin at the rate of 8 mg/kg body weight intramuscularly recorded the efficacy of 58.33%. Positive quarters treated with marbofloxacin single dose along with vit E, selenium and trisodium citrate recorded the efficacy of 80%. Hence, marbofloxacin along with vit E, selenium and trisodium citrate may be used effectively to treat subclinical mastitis in buffaloes.
Downloads
Metrics
References
Atakisi, O., H. Oral, E. Atakisi, O. Merhan, S.M. Pancarci, A. Ozcana, S. Marasli, B. Polat, A. Colak and S. Kaya. 2010. Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Res. Vet. Sci., 89(1): 10-13. DOI: 10.1016/j.rvsc.2010.01.008
Bauer, A.W., W.M.M. Kieby, J.C. Shrenis and M. Turck. 1966. Antibiotic susceptibity testing by a standardized single disc method. Am. J. Clin. Pathol., 45(4): 493-496. DOI: 10.1093/ajcp/45.4_ts.493
Buchnan, R.E. and N.E. Gibbon, 1984. Bergeys Manual of Determinative Bacteriology, 8th ed. The Williams and Wilkins Company Baltimore, USA.
Dua, K. 2001. Incidence, etiology and estimated economic losses due to mastitis in Punjab and in India-An update. Indian Dairyman, 53(10): 41-48.
Grandemange, E., P.A. Perrin, R.S. Richards and F. Woehrle. 2017. Efficacy of a single injection of marbofloxacin in the treatment of acute E. coli mastitis in lactating dairy cows. Revue. Med. Vet. Toulouse, 2017. 168(10-12): 219-228. Available on: https://www.revmedvet.com/2017/RMV168_219_228.pdf
Joshi, S. and S. Gokhale. 2006. Status of mastitis as an emerging disease in improved and periurban dairy farms in India, Ann. N. Y. Acad. Sci., 1081: 74-83. DOI: 10.1196/annals.1373.007
Khan, A.Z. and G. Muhammad. 2005. Quarterwise comparative prevalence of mastitis in buffaloes and crossbred cows. Pak. Vet. J., 25(1): 9-12. Available on: http://pvj.com.pk/pdf-files/25_1/9-12.pdf
Mahapatra, A., S. Panigrahi, R.C. Patra, M. Rout and S. Ganguly. 2018. A study on Bovine mastitis related oxidative stress along with therapeutic regimen. International Journal of Current Microbiology and Applied Sciences, 7(1): 247-256. DOI: 10.20546/ijcmas.2018.701.027
Mukherjee, R. 2007. Effect of bovine recombinant somatotropin on the occurrence of clinical mastitis in lactating buffaloes. Indian J. Vet. Med., 27(1): 60.
Patil, N.A., M.D. Harapanahalli, J.A. Mulia, S.V. Hosamani and B.K. Pugasheddi. 1995. Comparative study on prevalence and diagnosis of subclinical mastitis in cows and buffaloes. Indian J. Dairy Sci., 48: 478-479.
Patil, N.A., P.M. Rao and K.N.V. Sastry. 1996. Evaluation of electrical conductivity test in detecting subclinical udder infection. Indian J. Anim. Sci., 66(6): 575-576.
Pillet, F., C. Montreuil, O. Roy, E. Grandemange and F. Woehrle. 2013. Field experience with a single intravenous injection of marbofloxacin 16% in lactating dairy cows with acute clinical mastitis. Revue. Med. Vet. Toulouse, 164(12): 537-545. Available on: https://www.revmedvet.com/2013/RMV164_537_545.pdf
Rai, R.B., T. Damodaran, K. Dhama, S. Chakraborty, B. Singh, H. Ali, S. Rai and S. Mani. 2013. Evaluation studies on a tri-sodium citrate based novel formulation for prevention and treatment of bovine mastitis. International Journal of Current Research, 5(7): 1959-196.
Sandholm, M., L. Kaartinen and S. Pyorala. 1990. Bovine mastitis- Why does antibiotic therapy not always work? An overview. J. Vet. Pharmacol. Ther., 13(3): 248-260. DOI: 10.1111/j.1365-2885.1990.tb00774.x
Santoshi, P., P.S. Oberoi, M.N. Alhussien and A.K. Dang. 2018. Combined effect of trisodium citrate and vitamin E supplementation during the transition period on body weight and other production parameters in Sahiwal cows. Indian J. Dairy. Sci., 71(1): 78-83.
Schlam, O.W. and D.O. Noorlander. 1957. Experiments and observations leading to development of the California mastitis test. J. Am. Vet. Med. Assoc., 130(5): 199-204.
Sharma, A. and N. Sindhu. 2007. Occurrence of clinical and subclinical mastitis in buffaloes in the state of Haryana (India). Ital. J. Anim. Sci., 6: 965-967. DOI: 10.4081/ijas.2007.s2.965
Yousaf, A., I. Sarfaraz, M.A. Zafar, R.Z. Abbas, A. Hussain and A. Manzoor. 2010. Effect of treatment with tri-sodium citrate alone and in combination with levamisole HCL on total milk bacterial count in dairy buffalo suffering from subclinical mastitis. Revista Veterinaria, 21(1): 187-189.
.png){kind=logo}
