Evaluation of a multiplex PCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in ground beef marketed in Marajó island

Authors

  • Andrey Carlos do Sacramento de Oliveira Nutrition Undergraduate Course, Maurício de Nassau College, Ser Educacional Group, Belém, Pará, Brazil
  • Bárbara Cristina Amorim Ferreira Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil
  • Gabrielle Virgínia Lopes Ferreira Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil
  • Andréia Silva da Silva Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil
  • Milena Martins Andrade Fernandes Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil
  • Josyane Brasil da Silva Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil
  • Karla Fernanda Viana Mendonça Jardim Nutrition Undergraduate Course, Maurício de Nassau College, Ser Educacional Group, Belém, Pará, Brazil
  • Elieze Maciel Dias Nutrition Undergraduate Course, Maurício de Nassau College, Ser Educacional Group, Belém, Pará, Brazil
  • Fábio Pereira Leivas Leite Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil
  • Talita Bandeira Roos Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil
  • Carina Martins de Moraes Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil

DOI:

https://doi.org/10.56825/bufbu.2022.4143083

Keywords:

Bubalus bubalis, buffaloes, species identification, adulteration, fraud, bovine

Abstract

The present work intends to use a mPCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in samples commercially available on Marajó Island, Pará State, Brazil. For this purpose, a mPCR reaction with different concentrations of DNA and primers with different annealing temperatures was optimized. Next, 28 samples of ground meat marketed as being of bovine origin were collected and a mPCR for the determination of species and for the detection of Salmonella spp. were realized. The results demonstrated the efficiency of the proposed technique for identification of Bos taurus, Bubalus bubalis and Salmonella spp. We conclude that the proposed mPCR technique is efficient and that buffalo meat is marketed as bovine on the Island of Marajó and that such frauds violating the current legislation that normalizes the commercialization of ground beef.

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Author Biographies

Andrey Carlos do Sacramento de Oliveira, Nutrition Undergraduate Course, Maurício de Nassau College, Ser Educacional Group, Belém, Pará, Brazil

Andrey Carlos do Sacramento de Oliveira*

prof.andrey.sacramento@gmail.com

Bárbara Cristina Amorim Ferreira, Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil

Bárbara Cristina Amorim Ferreira

Gabrielle Virgínia Lopes Ferreira, Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil

Gabrielle Virgínia Lopes Ferreira

Andréia Silva da Silva, Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil

Andréia Silva da Silva

Milena Martins Andrade Fernandes, Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil

Milena Martins Andrade Fernandes

Josyane Brasil da Silva, Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil

Josyane Brasil da Silva

Karla Fernanda Viana Mendonça Jardim, Nutrition Undergraduate Course, Maurício de Nassau College, Ser Educacional Group, Belém, Pará, Brazil

Karla Fernanda Viana Mendonça Jardim

Elieze Maciel Dias, Nutrition Undergraduate Course, Maurício de Nassau College, Ser Educacional Group, Belém, Pará, Brazil

Elieze Maciel Dias

Fábio Pereira Leivas Leite, Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil

Fábio Pereira Leivas Leite

Talita Bandeira Roos, Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil

Talita Bandeira Roos

Carina Martins de Moraes, Food Hygiene and Quality Laboratory and Microbiology Laboratory, School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon, Federal University of Pará (Universidade Federal do Pará, UFPA), Castanhal, Brazil

Carina Martins de Moraes

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Published

2022-12-30

How to Cite

Oliveira, A. C. do S. de, Ferreira, B. C. A., Ferreira, G. V. L., Silva, A. S. da, Andrade Fernandes, M. M., Silva, J. B. da, … Moraes, C. M. de. (2022). Evaluation of a multiplex PCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in ground beef marketed in Marajó island. Buffalo Bulletin, 41(4), 699–706. https://doi.org/10.56825/bufbu.2022.4143083

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