Alteration of milk pH, somatic cell count (SCC), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activities in buffalo milk related to udder health status


  • Sani Nandi Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, West Tripura, India


Bubalus bubalis, buffaloes, alkaline phosphatase, lactate dehydrogenase, mastitis, pH, somatic cell count


The present study was designed to correlate the milk pH, somatic cell count (SCC), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activities in buffalo milk with the udder health status. A total of 60 (Sixty) buffaloes were randomly selected irrespective of their age, breed, feeding practice, stage and season of lactation from the herd belonging to Purnadhadi buffalo unit, PGIVAS, Teaching Veterinary Clinical complex, PGIVAS, Veterinary Polyclinic, Akola and also buffaloes of farmers in and around Akola. After thorough clinical examination of each udder, about 30 ml of milk was collected in sterilized, clean, dry plastic bottles and after careful screening by CMT, categorized in normal, sub- clinical (1+), sub- clinical (2+), sub- clinical (3+) and clinical group containing 12 animals in each group. The pH was measured using a digital pH meter (E.I. Model 101E) and SCC was calculated in freshly collected milk. LDH and ALP activities were measured by spectrophotometric method. The milk pH, SCC, LDH and ALP activities were found proportional to the severity of the udder infection as detected by the CMT reactions.


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How to Cite

Nandi, S. (2021). Alteration of milk pH, somatic cell count (SCC), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activities in buffalo milk related to udder health status. Buffalo Bulletin, 40(3), 443–450. Retrieved from



Original Article