The fertility of buffalo sperm (Bubalus bubalis) on three diluent types and three equilibration times
DOI:
https://doi.org/10.56825/bufbu.2022.4133171Keywords:
Bubalus bubalis, buffaloes, abnormality, acrosome intact, motility, membrane plasma integrity, pregnancy rateAbstract
This study objective was to determine the fertility of buffalo sperm in response to different types of diluent and equilibration times. The first research stage was arranged in completely randomized design with two factors, i.e., diluent types (tris egg yolk, andromed, and triladyl) and equilibration times (3 h, 4 h and 5 h). The second research stage was the fertility test of frozen semen that artificialy inseminated on 90 4-years-old buffalo-cows. The observed variables were the percentage of motility, abnormality, membrane plasma integrity, acrosome intact, recovery rate and pregnancy rate. The result showed that there was no interaction between diluent types and equilibration times on all observed variables. The best buffalo sperm fertility was produced from andromed and tris egg yolk compared to triladlil diluent. The best equilibration time for buffalo semen was 5 h rather than 4 h and 3 h.
Downloads
Download data is not yet available.
Metrics
Metrics Loading ...
References
Andrabi, S.M.H. 2009. Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reprod. Domest. Anim., 44(3): 552-569. DOI: 10.1111/j.1439-0531.2008.01240.x
Ansari, M.S., B. Rakha and S. Akhter. 2017. Cryopreservation of Nili-Ravi buffalo (Bubalus bubalis) semen in AndroMed extender; in vitro and in vivo evaluation. Reprod. Domest Anim., 52(6): 992-997. DOI: 10.1111/rda.13008
Ariantie, O., T. Yusuf, D. Sajuthi and R. Arifiantini. 2013. Pengaruh krioprotektan gliserol dan dimethilformamida dalam pembekuan semen kambing peranakan etawah menggunakan pengencer tris modifikasi. Jurnal Ilmu Ternak dan Veteriner, 18(4): 239-250.
Arifiantini, R. and B. Purwantara. 2010. Motility and viability of friesian holstein spermatozoa in three different extender stored at 5oC. Journal of the Indonesian Tropical Animal Agriculture, 35(4): 222-226. DOI: 10.14710/jitaa.35.4.222-226
Benson, J.D., E.J. Woods, E.M. Walters and J.K. Critser. 2012. The cryobiology of spermatozoa. Theriogenology, 78(8): 1682-1699. DOI: 10.1016/j.theriogenology.2012.06.007
Das, G.C., P.K. Das, S. Deori, H. Mazumdar, B.N. Bhattacharyya and A. Phookan. 2017. Seasonal variation in the characteristics of the swamp buffalo semen of northeast India. Buffalo Bull, 36(1): 215-220. Available on: https://kukrdb.lib.ku.ac.th/journal/BuffaloBulletin/search_detail/result/366256
Das, R.P. 1985. Assessment of spermatozoal function. Journal of Biosciences, 7(2): 245-255. Available on: https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.529.8497&rep=rep1&type=pdf
Eriani, K., N. Sari, M. Ihdina and Rosnizar. 2017. The effect of equilibration time on semen freezing of local swamp buffalo (Bubalus bubalis) with combination diluent of lactose and glycerol. Nusantara Bioscience, 9(1): 77-82.
Eriani, K., N. Sari, R. Rosnizar, D. Dasrul, S. Suhartono and M. Rizal. 2017. Cryopreservation of Aceh swamp buffalo (Bubalus bubalis) semen with combination of glycerol and lactose. Journal of Biology and Biology Education, 9(3): 409. DOI: 10.15294/biosaintifika.v9i3.11426
Febriani, G.D., H. Hamdan and J. Melia. 2014. The influence of time equilibration to the quality of the swamp buffalo (Bubalus bubalis) semen. Jurnal Medika Veterinaria, 8(1): 1-4.
Garner, D.L. and E.S.E. Hafez. 2016. Spermatozoa and seminal plasma, p. 96-109. In Reproduction in Farm Animals, Lippincott Williams and Wilkins, Baltimore, Maryland, USA.
Gordon, I. 2017. Reproductive Technologies in Farm Animals, Cabi, Wallingford, USA.
Hezavehei, M., M. Sharafi, H. Kouchesfahani, R. Mohseni, Henkel, A. Agarwal, V. Esmaeili and A. Shahverdi. 2018. Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. Reprod. BioMed., 37(3): 327-339. DOI: 10.1016/j.rbmo.2018.05.012
Holt, W. 2000. Basic aspects of frozen storage of semen. Anim. Reprod. Sci., 62(1-3): 3-22. DOI: 10.1016/s0378-4320(00)00152-4
Jainudeen, M.R. and E.S.E. Hafez. 2016. Cattle and buffalo, p. 157-171. In Reproduction in Farm Animals, Lippincott Williams and Wilkins, Baltimore, Maryland, USA.
Koonjaenak, S., V. Chanatinart, S. Aiumlamai, T. Pinyopumimintr and H. Rodriguez-Martinez. 2007. Seasonal variation in semen quality of swamp buffalo bulls (Bubalus bubalis) in Thailand. Asian J. Androl., 9(1): 92-101. DOI: 10.1111/j.1745-7262.2007.00230.x
Kumar, P., M. Saini, D. Kumar, A.K. Balhara, S.P. Yadav, P. Singh and P.S. Yadav. 2015. Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Anim. Reprod. Sci., 159: 38-45. DOI: 10.1016/j.anireprosci.2015.05.010
Manjunath, P. 2012. New insights into the understanding of the mechanism of sperm protection by extender components. Anim. Reprod. Sci., 9(4): 809-815.
Minitube. 2001. Certificate Andromed. Available on: https://www.minitube.com/pdf/index/13503-0200_Leaflet-AndroMed_en_171002.pdf
National Standardization Agency of Indonesia. 2008. Semen Beku Kerbau, Available on: https://id.scribd.com/document/345026647/SNI-4869-2-2008-Semen-Beku-Kerbau
Naz, S., M. Umair and S. Iqbal. 2018. Comparison of tris egg yolk-based, triladyl and optixell extender on post-thaw quality, kinematics and in vivo fertility of Nili ravi buffalo (Bubalus bubalis) bull spermatozoa. Andrologia, 50(8): 13063.
Shah, S.A., S.M. Andrabi and I.Z. Qureshi. 2016. Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Andrology, 4(5): 972-976. DOI: 10.1111/andr.12214
Shahverdi, A., A. Rastegarnia and T. Rezaei. 2014. Effect of extender and equilibration time on post thaw motility and chromatin structure of buffalo bull (Bubalus bubalis) spermatozoa. Cell J., 16(3): 279-288.
Singh, I. and A.K. Balhara. 2016. New approaches in buffalo artificial insemination programs with special reference to India. Theriogenology, 86(1): 194-199. DOI: 10.1016/j.theriogenology.2016.04.031
Steel, R.G.D., D. George, J.H. Torrie and B. Sumantri. 1991. Prinsip dan prosedur statistika : Suatu pendekatan biometrik. Gramedia Pustaka Utama, Jakarta, Indonesia.
Yulnawati, M. Gunawan, H. Maheswari, M. Rizal, Herdis and A. Boediono. 2010. Quality of epididymal and ejaculated sperms of spotted buffalo in dextrose supplemented extender. HAYATI Journal of Bioscience, 17(1): 27-30. DOI: 10.4308/hjb.17.1.27
Ansari, M.S., B. Rakha and S. Akhter. 2017. Cryopreservation of Nili-Ravi buffalo (Bubalus bubalis) semen in AndroMed extender; in vitro and in vivo evaluation. Reprod. Domest Anim., 52(6): 992-997. DOI: 10.1111/rda.13008
Ariantie, O., T. Yusuf, D. Sajuthi and R. Arifiantini. 2013. Pengaruh krioprotektan gliserol dan dimethilformamida dalam pembekuan semen kambing peranakan etawah menggunakan pengencer tris modifikasi. Jurnal Ilmu Ternak dan Veteriner, 18(4): 239-250.
Arifiantini, R. and B. Purwantara. 2010. Motility and viability of friesian holstein spermatozoa in three different extender stored at 5oC. Journal of the Indonesian Tropical Animal Agriculture, 35(4): 222-226. DOI: 10.14710/jitaa.35.4.222-226
Benson, J.D., E.J. Woods, E.M. Walters and J.K. Critser. 2012. The cryobiology of spermatozoa. Theriogenology, 78(8): 1682-1699. DOI: 10.1016/j.theriogenology.2012.06.007
Das, G.C., P.K. Das, S. Deori, H. Mazumdar, B.N. Bhattacharyya and A. Phookan. 2017. Seasonal variation in the characteristics of the swamp buffalo semen of northeast India. Buffalo Bull, 36(1): 215-220. Available on: https://kukrdb.lib.ku.ac.th/journal/BuffaloBulletin/search_detail/result/366256
Das, R.P. 1985. Assessment of spermatozoal function. Journal of Biosciences, 7(2): 245-255. Available on: https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.529.8497&rep=rep1&type=pdf
Eriani, K., N. Sari, M. Ihdina and Rosnizar. 2017. The effect of equilibration time on semen freezing of local swamp buffalo (Bubalus bubalis) with combination diluent of lactose and glycerol. Nusantara Bioscience, 9(1): 77-82.
Eriani, K., N. Sari, R. Rosnizar, D. Dasrul, S. Suhartono and M. Rizal. 2017. Cryopreservation of Aceh swamp buffalo (Bubalus bubalis) semen with combination of glycerol and lactose. Journal of Biology and Biology Education, 9(3): 409. DOI: 10.15294/biosaintifika.v9i3.11426
Febriani, G.D., H. Hamdan and J. Melia. 2014. The influence of time equilibration to the quality of the swamp buffalo (Bubalus bubalis) semen. Jurnal Medika Veterinaria, 8(1): 1-4.
Garner, D.L. and E.S.E. Hafez. 2016. Spermatozoa and seminal plasma, p. 96-109. In Reproduction in Farm Animals, Lippincott Williams and Wilkins, Baltimore, Maryland, USA.
Gordon, I. 2017. Reproductive Technologies in Farm Animals, Cabi, Wallingford, USA.
Hezavehei, M., M. Sharafi, H. Kouchesfahani, R. Mohseni, Henkel, A. Agarwal, V. Esmaeili and A. Shahverdi. 2018. Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. Reprod. BioMed., 37(3): 327-339. DOI: 10.1016/j.rbmo.2018.05.012
Holt, W. 2000. Basic aspects of frozen storage of semen. Anim. Reprod. Sci., 62(1-3): 3-22. DOI: 10.1016/s0378-4320(00)00152-4
Jainudeen, M.R. and E.S.E. Hafez. 2016. Cattle and buffalo, p. 157-171. In Reproduction in Farm Animals, Lippincott Williams and Wilkins, Baltimore, Maryland, USA.
Koonjaenak, S., V. Chanatinart, S. Aiumlamai, T. Pinyopumimintr and H. Rodriguez-Martinez. 2007. Seasonal variation in semen quality of swamp buffalo bulls (Bubalus bubalis) in Thailand. Asian J. Androl., 9(1): 92-101. DOI: 10.1111/j.1745-7262.2007.00230.x
Kumar, P., M. Saini, D. Kumar, A.K. Balhara, S.P. Yadav, P. Singh and P.S. Yadav. 2015. Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Anim. Reprod. Sci., 159: 38-45. DOI: 10.1016/j.anireprosci.2015.05.010
Manjunath, P. 2012. New insights into the understanding of the mechanism of sperm protection by extender components. Anim. Reprod. Sci., 9(4): 809-815.
Minitube. 2001. Certificate Andromed. Available on: https://www.minitube.com/pdf/index/13503-0200_Leaflet-AndroMed_en_171002.pdf
National Standardization Agency of Indonesia. 2008. Semen Beku Kerbau, Available on: https://id.scribd.com/document/345026647/SNI-4869-2-2008-Semen-Beku-Kerbau
Naz, S., M. Umair and S. Iqbal. 2018. Comparison of tris egg yolk-based, triladyl and optixell extender on post-thaw quality, kinematics and in vivo fertility of Nili ravi buffalo (Bubalus bubalis) bull spermatozoa. Andrologia, 50(8): 13063.
Shah, S.A., S.M. Andrabi and I.Z. Qureshi. 2016. Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Andrology, 4(5): 972-976. DOI: 10.1111/andr.12214
Shahverdi, A., A. Rastegarnia and T. Rezaei. 2014. Effect of extender and equilibration time on post thaw motility and chromatin structure of buffalo bull (Bubalus bubalis) spermatozoa. Cell J., 16(3): 279-288.
Singh, I. and A.K. Balhara. 2016. New approaches in buffalo artificial insemination programs with special reference to India. Theriogenology, 86(1): 194-199. DOI: 10.1016/j.theriogenology.2016.04.031
Steel, R.G.D., D. George, J.H. Torrie and B. Sumantri. 1991. Prinsip dan prosedur statistika : Suatu pendekatan biometrik. Gramedia Pustaka Utama, Jakarta, Indonesia.
Yulnawati, M. Gunawan, H. Maheswari, M. Rizal, Herdis and A. Boediono. 2010. Quality of epididymal and ejaculated sperms of spotted buffalo in dextrose supplemented extender. HAYATI Journal of Bioscience, 17(1): 27-30. DOI: 10.4308/hjb.17.1.27
Downloads
Published
2022-09-29
How to Cite
-, Y., Handoko, J., Rodiallah, M., & Arman, C. (2022). The fertility of buffalo sperm (Bubalus bubalis) on three diluent types and three equilibration times. Buffalo Bulletin, 41(3), 521–529. https://doi.org/10.56825/bufbu.2022.4133171
Issue
Section
Original Article
.png){kind=logo}
