Pathological studies on spontaneous urinary bladder lesions in Indian buffaloes
DOI:
https://doi.org/10.56825/bufbu.2023.4223585Keywords:
Bubalus bubalis, buffaloes, epithelial abnormalities, pathology, spontaneous, urinary bladderAbstract
The present investigation describes the spontaneous pathological lesions in urinary bladders (n = 177) of slaughtered buffaloes collected from Northern India. Gross examination revealed congestion, haemorrhages, oedematous changes and thickening of mucosa. Microscopically, epithelial abnormalities, inflammatory and neoplastic conditions were noticed. Epithelial abnormalities included epithelial hyperplasia, von Brunn’s nests, cystitis cystica and cystitis glandularis. Inflammatory conditions such as congestion, haemorrhages and cystitis were noticed. Cystitis was classified as lymphocytic, plasmo-lymphocytic, follicular and interstitial types. However, only single case of haemangiosarcoma was observed. Polymerase chain reaction revealed the presence of DNA of only BPV-1 in one sample out of 21, while one sample showed the presence of DNA of both BPV-1 and BPV-2. On immunohistochemistry, Ki-67 expression was observed only in haemangiosarcoma and one case of plasmo-lymphocytic cystitis with immunostaining restricted to nuclei. COX-2 immunostaining was observed in one case each of flat hyperplasia, von Brunn’s nest, haemangiosarcoma and two cases of plasmo-lymphocytic cystitis. Among epithelial abnormalities, COX-2 immunostaining was restricted to epithelial component of the lesions, and it was diffused cytoplasmic type with occasional perinuclear staining particularly in the cytoplasmic membrane. However, in haemangiosarcoma the COX-2 immunoreactivity was noticed in epithelial as well as connective tissue stroma. In conclusion, it may be stated that urinary bladders of slaughtered buffaloes showed a number of epithelial abnormalities, inflammatory conditions and occasionally neoplasms. Further studies to explore the infectious as well as non-infectious etiologies should be undertaken.
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References
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Ristimaki, A., O. Nieminen, K. Saukkonen, K. Hotakainen, S. Nordling and C. Haglund. 2001. Expression of cyclooxygenase-2 in human transitional cell carcinoma of the urinary bladder. Am. J. Pathol., 158(3): 849-853. DOI: 10.1016/S0002-9440(10)64033-3
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Somvanshi, R. 2011. Papillomatosis in buffaloes: A less-known disease. Transbound. Emerg. Dis., 58(4): 327-332. DOI: 10.1111/j.1865-1682.2011.01211.x
Somvanshi, R., S. Pathania, N. Nagarajan, K. Pangty and P. Kumar. 2012. Pathological study of non-neoplastic urinary bladder lesions in cattle and buffaloes: A preliminary report. Trop. Anim. Health Pro., 44(4): 855-861. DOI: 10.1007/s11250-011-9978-y
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Wadhwa, P., A.K. Goswami, K. Joshi and S.K. Sharma. 2005. Cyclooxygenase-2 expression increases with the stage and grade in transitional cell carcinoma of the urinary bladder. Int. Urol. Nephrol., 37(1): 47-53. DOI: 10.1007/s11255-004-4699-z
Woods, A.L., P.A. Hall, N.A. Shepherd, A.M. Hanby, N.H. Waseem, D.P. Lane and DA Levison. 1991. The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis. Histopathology, 19(1): 21-27. DOI: 10.1111/j.1365-2559.1991.tb00890.x
Yaguiu, A., C. de Carvalho, A.C. de Freitas, L.G.B. Goes, M.L.Z. Dagli, E.H. Birgel Jr, W. Becak and R. de Cassia Stocco dos Santos. 2006. Papillomatosis in cattle: In situ detection of bovine papillomavirus DNA sequences in reproductive tissues. Brazilian Journal of Morphological Sciences, 23: 525-529.
Aydin, Y. and I.A. Ozkul. 1995. Non-neoplastic findings of the urinary bladder in cattle and water buffalo in the Black region of Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 42: 189-192.
Banerjee, G.C. 1998. Buffalo, p. 697-727. In A Text Book of Animal Husbandry. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, India.
Borzacchiello, G., G. Lovane, M.L. Marcante, F. Poggiali, F. Roperto, S. Roperto and A. Venuti. 2003. Presence of bovine papillomavirus type 2 DNA and expression of the viral oncoprotein E5 in naturally occurring urinary bladder tumours in cows. J. Gen. Virol., 84(11): 2921-2926. DOI: 10.1099/vir.0.19412-0
Carvalho, T., D. Naydan, T. Nunes, C. Pinto and M.C. Peleteiro. 2009. Immunohistochemical evaluation of vascular urinary bladder tumors from cows with enzootic hematuria. Vet. Pathol., 46(2): 211-221. DOI: 10.1354/vp.46-2-211
Deshmukh, S., H.S. Banga, K.S. Kwatra, N.D. Singh, P.D. Gadhave and R.S. Brar. 2010. Immunohistochemical study on spontaneous transitional cell carcinoma of urinary bladder in an Indian water buffalo (Bubalus bubalis). Indian Journal of Veterinary Pathology, 34: 113-116.
Dore, M., I. Lanthier and J. Sirois. 2003. Cyclooxygenase-2 expression in canine mammary tumors. Vet. Pathol., 40(2): 207-212. DOI: 10.1354/vp.40-2-207
Dore, M. 2011. Cyclooxygenase-2 expression in animal cancers. Vet. Pathol., 48(1): 254-265. DOI: 10.1177/0300985810379434
Fagiolo, A., C. Roncoroni, O. Lai and A. Borghese. 2005. Buffalo pathologies, p. 249-296. In Borghese, A. (edn.) Buffalo Production and Research, Food and Agriculture Organization of the United Nations, Rome, Italy.
Herenda, D., T.W. Dukes and T.E. Feltmate. 1990. An abattoir survey of urinary bladder lesions in cattle. Canadian Vet. J., 31(7): 515-518.
Khan, K.N.M., K. Stanfield, D. Trajkovic and R.K. Harris. 2000. Cyclooxygenase-2 expression in inflammatory lung lesions of nonhuman primates. Vet. Pathol., 37: 512-516. DOI: 10.1354/vp.37-5-512a
Kumar, P., S. Pathania, B. Jangir and R. Somvanshi. 2014. Expression of certain cancer biomarkers in urinary bladder tumors/lesions of cattle and buffaloes. Indian Journal of Veterinary Pathology, 38(3): 143-148. DOI: 10.5958/0973-970X.2014.01161.4
Leishangthem, G.D. 2006. Etio-pathological studies on bovine papillomatosis and its combined effects on fern fed hamsters. Ph.D. Thesis, Indian Veterinary Research Institute Deemed University, Izatnagar, Uttar Pradesh, India.
Li, G., T. Yang, L. Li, J. Yan, Y. Zeng, J. Yu and Y. Zahang. 2004. Cyclooxygenase-2 parallels invasive depth and increased MVD in transitional cell carcinoma. Colloid. Surface. B., 37(1-2): 15-19. DOI: 10.1016/j.colsurfb.2004.05.011
Luna, L.G. 1968. Manual of Histological Staining Methods of The Armed Force Institute of Pathology, 3rd ed. Mc Graw Hill Book Co., London, UK. 258p.
Mckenzie, R.A. 1978. Bovine enzootic haematuria in Queensland. Aust. Vet. J., 54: 61-64. DOI: 10.1111/j.1751-0813.1978.tb00344.x
Nagarajan, N. 2011. BPV-Bracken fern interaction in buffaloes and laboratory model hamsters: Molecular and clinico-pathological studies. M.V.Sc. Thesis, Indian Veterinary Research Institute Deemed University, Izatnagar, Uttar Pradesh, India.
Ozkul, I.A. and Y. Aydin. 1996. Tumours of the urinary bladder in cattle and water buffalo in the black sea region of Turkey. Brit. Vet. J., 152(4): 473-475. DOI: 10.1016/S0007-1935(96)80041-8
Pangty, K. 2009. Molecular diagnosis of bovine papillomatosis/enzootic bovine haematuria and assessment of BPV-2 hamster tumour model for immunoprophylaxis/ anti-tumour therapeutics. M.V.Sc. Thesis, Indian Veterinary Research Institute Deemed University, Izatnagar, Uttar Pradesh, India.
Pathania, S., K. Dhama, G. Saikumar, S. Shahi and R. Somvanshi. 2012. Detection and quantification of bovine papilloma virus type 2 (BPV-2) by real-time PCR in urine and urinary bladder lesions in enzootic bovine haematuria (EBH)-affected cows. Transbound. Emerg. Dis., 59(1): 79-84. DOI: 10.1111/j.1865-1682.2011.01248.x
Radostits, O.M., C.C. Gay, K.W. Hinchcliff and P.D. Constable. 2007. Diseases of the urinary system, p. 543-573. In Veterinary Medicine: A Textbook of The Diseases of Cattle, Horses, Sheep, Pigs and Goats, Saunders Elsevier, Edinburgh, London, UK.
Ristimaki, A., O. Nieminen, K. Saukkonen, K. Hotakainen, S. Nordling and C. Haglund. 2001. Expression of cyclooxygenase-2 in human transitional cell carcinoma of the urinary bladder. Am. J. Pathol., 158(3): 849-853. DOI: 10.1016/S0002-9440(10)64033-3
Scholzen, T. and J. Gerdes. 2000. The Ki-67 protein: From the known and the unknown. J. Cell. Physiol., 182(3): 311-322. DOI: 10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9
Sledge, D.G., D.J. Patrick, S.D. Fitzgerzld, Y. Xie and M. Kiupel. 2015. Differences in expression of uroplakin III, cytokeratin 7, and cyclooxygenase-2 in canine proliferative urothelial lesions of the urinary bladder. Vet. Pathol., 52(1): 74-82. DOI: 10.1177/0300985814522819
Somvanshi, R. 2011. Papillomatosis in buffaloes: A less-known disease. Transbound. Emerg. Dis., 58(4): 327-332. DOI: 10.1111/j.1865-1682.2011.01211.x
Somvanshi, R., S. Pathania, N. Nagarajan, K. Pangty and P. Kumar. 2012. Pathological study of non-neoplastic urinary bladder lesions in cattle and buffaloes: A preliminary report. Trop. Anim. Health Pro., 44(4): 855-861. DOI: 10.1007/s11250-011-9978-y
Sreelekshmy, M., R.B. Bind, M.M. Nilakanth, C. Balachandran and R. Somvanshi. 2017. Studies on spontaneous pathological lesions in bovine and bubaline urinary bladders with reference to detection of Bovine Papillomaviruses (BPVs) and H-ras biomarker. Indian Journal of Veterinary Pathology, 41(4): 239-244. DOI: 10.5958/0973-970X.2017.00059.1
Wadhwa, P., A.K. Goswami, K. Joshi and S.K. Sharma. 2005. Cyclooxygenase-2 expression increases with the stage and grade in transitional cell carcinoma of the urinary bladder. Int. Urol. Nephrol., 37(1): 47-53. DOI: 10.1007/s11255-004-4699-z
Woods, A.L., P.A. Hall, N.A. Shepherd, A.M. Hanby, N.H. Waseem, D.P. Lane and DA Levison. 1991. The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis. Histopathology, 19(1): 21-27. DOI: 10.1111/j.1365-2559.1991.tb00890.x
Yaguiu, A., C. de Carvalho, A.C. de Freitas, L.G.B. Goes, M.L.Z. Dagli, E.H. Birgel Jr, W. Becak and R. de Cassia Stocco dos Santos. 2006. Papillomatosis in cattle: In situ detection of bovine papillomavirus DNA sequences in reproductive tissues. Brazilian Journal of Morphological Sciences, 23: 525-529.
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2023-06-30
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Jangir, B. L., & Somvanshi, R. (2023). Pathological studies on spontaneous urinary bladder lesions in Indian buffaloes. Buffalo Bulletin, 42(2), 277–289. https://doi.org/10.56825/bufbu.2023.4223585
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