Differentiation of cattle and buffalo meat using multiplex polymerase chain reaction
DOI:
https://doi.org/10.56825/bufbu.2023.4213617Keywords:
Bubalus bubalis, buffaloes, adulteration, labelling, mitochondrial D loop, primer, speciationAbstract
Authentication of origin of animal species is of paramount importance owing to economic, social, religious, forensic and public health considerations. In the present study, a multiplex polymerase chain reaction (mPCR) was developed by designing unique species-specific forward and a common reverse primers flanking mitochondrial D-loop region for the authentication of cattle (Bos indicus / B. taurus) and buffalo (Bubalus bubalis) species. Novel mPCR amplified 382 bp product specific to cattle and 336 bp specific to buffalo. Possibility of cross amplification in other species was rules out by testing as many as 25 other animal species. The novel mPCR assay developed in this study for the authentic identification and differentiation of meats of cattle and buffalo could be applied for the differentiation of samples derived from cattle and buffalo in diagnostic laboratories thereby ensuring prompt labelling of products, protecting consumer sentiments and enforcement of laws.
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References
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Dalmasso, A., T. Civera, F.L. Neve and M.T. Bottero. 2011. Simultaneous detection of cow and buffalo milk in mozzarella cheese by Real-Time PCR assay. Food Chem., 124(1): 362-366. DOI: 10.1016/j.foodchem.2010.06.017
De, S., B. Brahma, S. Polley, A. Mukherjee, D. Banerjee, M. Gohaina, K.P. Singh, R. Singh, T.K. Datta and S.L. Goswami. 2011. Simplex and duplex PCR assays for species specific identification of cattle and buffalo milk and cheese. Food Control, 22(5): 690-696. DOI: 10.1016/j.foodcont.2010.09.026
El-Rady, A. and M. Sayed. 2006. Identification of milk source by polymerase chain reaction-restriction fragment length polymorphism analysis. J. Rapid Meth. Aut. Mic., 14: 146-155. DOI: 10.1111/j.1745-4581.2006.00036.x
Girish, P.S., A.S.R. Anjaneyulu, K.N. Viswas, M. Anand, N. Rajkumar, B.M. Shivakumar and B. Sharma. 2004. Sequence analysis of mitochondrial 12S rRNA gene can identify meat species. Meat Sci., 66(3): 551-556. DOI: 10.1016/S0309-1740(03)00158-X
Girish, P.S., A.S.R. Anjaneyulu, K.N. Viswas, B.M. Shivakumar, M. Anand, M. Patel and B. Sharma. 2005. Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene. Meat Sci., 70(1): 107-112. DOI: 10.1016/j.meatsci.2004.12.004
Girish, P.S., S.B. Barbuddhe, A. Kumari, D.B. Rawool, N.S. Karabasanavar, M. Muthukumar and S. Vaithiyanathan. 2020. Rapid detection of pork using Alkaline Lysis-Loop Mediated Isothermal Amplification (AL-LAMP) technique. Food Control, 110: 107015. DOI: 10.1016/j.foodcont.2019.107015
Guha, S. and V.K. Kashyap. 2005. Development of novel heminested PCR assays based on mitochondrial 16s rRNA gene for identification of seven pecora species. BMC Genetics, 6: 42. DOI: 10.1186/1471-2156-6-42
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Karabasanavar, N.S., S.P. Singh, S.N. Shebannavar, A.M. Kotresh, P.S. Girish and P.V. Umapathi. 2010. Identification of avian species using polymerase chain reaction and sequence analysis of mitochondrial 12S rRNA gene. Vet. Arhiv., 80: 653-661. Available on: https://hrcak.srce.hr/file/93152
Karabasanavar, N.S., S.P. Singh, V. Umapathi, P.S. Girish, S.N. Shebannavar and D. Kumar. 2011c. Authentication of carabeef (water buffalo, Bubalus bubalis) using highly specific polymerase chain reaction. Eur. Food Res. Technol., 233(6): 985-989. DOI: 10.1007/s00217-011-1583-9
Karabasanavar, N.S., S.P. Singh, V. Umapathi, D. Kumar, G. Patil and S.N. Shebannavar. 2011a. A highly specific PCR assay for identification of raw and heat treated mutton (Ovis aries). Small Ruminant Res., 100(2-3): 153-158. DOI: 10.1016/j.smallrumres.2011.07.009
Karabasanavar, N.S., S.P. Singh, V. Umapathi, D. Kumar and S.N. Shebannavar. 2011b. Identification of goat meat using highly species-specific polymerase chain reaction. J. Food Quality, 34(2): 142-149. DOI: 10.1111/j.1745-4557.2011.00376.x
Kumar, D., S.P. Singh, R. Singh and N.S. Karabasanavar. 2011. A highly specific PCR assay for identification of goat (Capra hircus) meat. Small Ruminamt Res., 97(1-3): 76-78. DOI: 10.1016/j.smallrumres.2011.01.013
Martin, I., T. Garcia, V. Fajardo, L. Calleja, M. Rojas, P.E. Hernandez, I. Gonzalez and R. Martin. 2007. Mitochondrial markers for the detection of four duck species and the specific identification of Muscovy duck in meat mixtures using the polymerase chain reaction. Meat Sci., 76(4): 721-729. DOI: 10.1016/j.meatsci.2007.02.013
Martinez, I. and I.M. Yman. 1998. Species identification in meat products by RAPD analysis. Food Res. Int., 31(6-7): 459-466. DOI: 10.1016/S0963-9969(99)00013-7
Matsunaga, T., K. Chikuni, R. Tanabe, S. Muroya, K. Shibata, J. Yamada and Y. Shinmura. 1999. A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Sci., 51(2): 143-148. DOI: 10.1016/s0309-1740(98)00112-0
Parson, W., K. Pegoraro, H. Neiderstatter, M. Foger and M. Steinlechner. 2000. Species identification by means of the cytochrome b gene. Int. J. Legal Med., 114(1-2): 23-28. DOI: 10.1007/s004140000134
Prusiner, S.B. 1994. Biology and genetics of prion diseases. Annu. Rev. Microbiol., 48: 655-686. DOI: 10.1146/annurev.mi.48.100194.003255
Rajapaksha, W.R.A.K.J.S., I.D.S.I.P. Thilakarantne, A.D.N. Chandrasiri and T.B. Niroshan. 2003. Development of PCR assay for identification of buffalo meat. Asian Austral. J. Anim., 16: 1046-1048. Available on: http://www.koreascience.or.kr/article/JAKO200310103489228.pdf
Rodriguez, M.A., T. Garcia, I. Gonzalez, L. Asensio, A. Ferandez, E. Lobo, P.E. Hernandez and R. Martin. 2001. Identification of goose (Anser anser) and mule duck (Anas platyrhynchos x Cairina moschata) foie gras by multiplex Polymerase Chain Reaction amplification of the 5S rDNA gene. J. Agr. Food Chem., 49(6): 2717-2721. DOI: 10.1021/jf0014765
Sambrook, J. and D.W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbour Laboratory Press, New York, USA.
Sawyer, J., C. Wood, D. Shanahan, S. Gout and D. McDowell. 2003. Real-time PCR for quantitative meat species testing. Food Control, 14(8): 579-583. DOI: 10.1016/S0956-7135(02)00148-2
Takeda, K., A. Onishi, N. Ishida, K. Kawakami, M. Komatsu and S. Inumaru. 1995. SSCP analysis of pig mitochondrial DNA d-loop region polymorphism. Anim. Genet., 26(5): 321-326. DOI: 10.1111/j.1365-2052.1995.tb02666.x
Wang, Q., X. Zhang, H.Y. Zhang, J. Zhang, G.Q. Chen, D.H. Zhao, H.P. Mab and W.J. Liao. 2010. Identification of 12 animal species meat by T-RFLP on the 12S rRNA gene. Meat Sci., 85(2): 265-269. DOI: 10.1016/j.meatsci.2010.01.010
Werfel, S.J., S.K. Cooke and H.A. Sampson. 1997. Clinical reactivity to beef in children allergic to cow’s milk. J. Allergy Clin. Immun., 99(3): 293-300. DOI: 10.1016/s0091-6749(97)70045-9
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Re., 18(22): 6531-6535. DOI: 10.1093/nar/18.22.6531
Bataille, M., K. Crainic, M. Leterreux, M. Durigon and P. de Mazancourt. 1999. Multiplex amplification of mitochondrial DNA for human and species identification in forensic evaluation. Forensic Sci. Int., 99(3):165-70. DOI: 10.1016/s0379-0738(98)00185-6
Brodmann, P.D. and D. Moor. 2003. Sensitive and semi-quantitative TaqManTM real-time polymerase chain reaction systems for the detection of beef (Bos taurus) and the detection of the family Mammalia in food and feed. Meat Sci., 65(1): 599-607.
Dalmasso, A., T. Civera, F.L. Neve and M.T. Bottero. 2011. Simultaneous detection of cow and buffalo milk in mozzarella cheese by Real-Time PCR assay. Food Chem., 124(1): 362-366. DOI: 10.1016/j.foodchem.2010.06.017
De, S., B. Brahma, S. Polley, A. Mukherjee, D. Banerjee, M. Gohaina, K.P. Singh, R. Singh, T.K. Datta and S.L. Goswami. 2011. Simplex and duplex PCR assays for species specific identification of cattle and buffalo milk and cheese. Food Control, 22(5): 690-696. DOI: 10.1016/j.foodcont.2010.09.026
El-Rady, A. and M. Sayed. 2006. Identification of milk source by polymerase chain reaction-restriction fragment length polymorphism analysis. J. Rapid Meth. Aut. Mic., 14: 146-155. DOI: 10.1111/j.1745-4581.2006.00036.x
Girish, P.S., A.S.R. Anjaneyulu, K.N. Viswas, M. Anand, N. Rajkumar, B.M. Shivakumar and B. Sharma. 2004. Sequence analysis of mitochondrial 12S rRNA gene can identify meat species. Meat Sci., 66(3): 551-556. DOI: 10.1016/S0309-1740(03)00158-X
Girish, P.S., A.S.R. Anjaneyulu, K.N. Viswas, B.M. Shivakumar, M. Anand, M. Patel and B. Sharma. 2005. Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene. Meat Sci., 70(1): 107-112. DOI: 10.1016/j.meatsci.2004.12.004
Girish, P.S., S.B. Barbuddhe, A. Kumari, D.B. Rawool, N.S. Karabasanavar, M. Muthukumar and S. Vaithiyanathan. 2020. Rapid detection of pork using Alkaline Lysis-Loop Mediated Isothermal Amplification (AL-LAMP) technique. Food Control, 110: 107015. DOI: 10.1016/j.foodcont.2019.107015
Guha, S. and V.K. Kashyap. 2005. Development of novel heminested PCR assays based on mitochondrial 16s rRNA gene for identification of seven pecora species. BMC Genetics, 6: 42. DOI: 10.1186/1471-2156-6-42
Janssen, F.W., G.H. Hagele, J.B. Buntjer and J.A. Lenstra. 1998. Species identification in meat using PCR- generated satellite probes. J. Ind. Microbiol. Biot., 21: 115-120. Available on: https://link.springer.com/content/pdf/10.1038/sj.jim.2900541.pdf
Karabasanavar, N.S., S.P. Singh, S.N. Shebannavar, A.M. Kotresh, P.S. Girish and P.V. Umapathi. 2010. Identification of avian species using polymerase chain reaction and sequence analysis of mitochondrial 12S rRNA gene. Vet. Arhiv., 80: 653-661. Available on: https://hrcak.srce.hr/file/93152
Karabasanavar, N.S., S.P. Singh, V. Umapathi, P.S. Girish, S.N. Shebannavar and D. Kumar. 2011c. Authentication of carabeef (water buffalo, Bubalus bubalis) using highly specific polymerase chain reaction. Eur. Food Res. Technol., 233(6): 985-989. DOI: 10.1007/s00217-011-1583-9
Karabasanavar, N.S., S.P. Singh, V. Umapathi, D. Kumar, G. Patil and S.N. Shebannavar. 2011a. A highly specific PCR assay for identification of raw and heat treated mutton (Ovis aries). Small Ruminant Res., 100(2-3): 153-158. DOI: 10.1016/j.smallrumres.2011.07.009
Karabasanavar, N.S., S.P. Singh, V. Umapathi, D. Kumar and S.N. Shebannavar. 2011b. Identification of goat meat using highly species-specific polymerase chain reaction. J. Food Quality, 34(2): 142-149. DOI: 10.1111/j.1745-4557.2011.00376.x
Kumar, D., S.P. Singh, R. Singh and N.S. Karabasanavar. 2011. A highly specific PCR assay for identification of goat (Capra hircus) meat. Small Ruminamt Res., 97(1-3): 76-78. DOI: 10.1016/j.smallrumres.2011.01.013
Martin, I., T. Garcia, V. Fajardo, L. Calleja, M. Rojas, P.E. Hernandez, I. Gonzalez and R. Martin. 2007. Mitochondrial markers for the detection of four duck species and the specific identification of Muscovy duck in meat mixtures using the polymerase chain reaction. Meat Sci., 76(4): 721-729. DOI: 10.1016/j.meatsci.2007.02.013
Martinez, I. and I.M. Yman. 1998. Species identification in meat products by RAPD analysis. Food Res. Int., 31(6-7): 459-466. DOI: 10.1016/S0963-9969(99)00013-7
Matsunaga, T., K. Chikuni, R. Tanabe, S. Muroya, K. Shibata, J. Yamada and Y. Shinmura. 1999. A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Sci., 51(2): 143-148. DOI: 10.1016/s0309-1740(98)00112-0
Parson, W., K. Pegoraro, H. Neiderstatter, M. Foger and M. Steinlechner. 2000. Species identification by means of the cytochrome b gene. Int. J. Legal Med., 114(1-2): 23-28. DOI: 10.1007/s004140000134
Prusiner, S.B. 1994. Biology and genetics of prion diseases. Annu. Rev. Microbiol., 48: 655-686. DOI: 10.1146/annurev.mi.48.100194.003255
Rajapaksha, W.R.A.K.J.S., I.D.S.I.P. Thilakarantne, A.D.N. Chandrasiri and T.B. Niroshan. 2003. Development of PCR assay for identification of buffalo meat. Asian Austral. J. Anim., 16: 1046-1048. Available on: http://www.koreascience.or.kr/article/JAKO200310103489228.pdf
Rodriguez, M.A., T. Garcia, I. Gonzalez, L. Asensio, A. Ferandez, E. Lobo, P.E. Hernandez and R. Martin. 2001. Identification of goose (Anser anser) and mule duck (Anas platyrhynchos x Cairina moschata) foie gras by multiplex Polymerase Chain Reaction amplification of the 5S rDNA gene. J. Agr. Food Chem., 49(6): 2717-2721. DOI: 10.1021/jf0014765
Sambrook, J. and D.W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbour Laboratory Press, New York, USA.
Sawyer, J., C. Wood, D. Shanahan, S. Gout and D. McDowell. 2003. Real-time PCR for quantitative meat species testing. Food Control, 14(8): 579-583. DOI: 10.1016/S0956-7135(02)00148-2
Takeda, K., A. Onishi, N. Ishida, K. Kawakami, M. Komatsu and S. Inumaru. 1995. SSCP analysis of pig mitochondrial DNA d-loop region polymorphism. Anim. Genet., 26(5): 321-326. DOI: 10.1111/j.1365-2052.1995.tb02666.x
Wang, Q., X. Zhang, H.Y. Zhang, J. Zhang, G.Q. Chen, D.H. Zhao, H.P. Mab and W.J. Liao. 2010. Identification of 12 animal species meat by T-RFLP on the 12S rRNA gene. Meat Sci., 85(2): 265-269. DOI: 10.1016/j.meatsci.2010.01.010
Werfel, S.J., S.K. Cooke and H.A. Sampson. 1997. Clinical reactivity to beef in children allergic to cow’s milk. J. Allergy Clin. Immun., 99(3): 293-300. DOI: 10.1016/s0091-6749(97)70045-9
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Re., 18(22): 6531-6535. DOI: 10.1093/nar/18.22.6531
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Published
2023-03-31
How to Cite
Karabasanavar, N. S., Shivanagowda, G. P., & Singh, S. P. (2023). Differentiation of cattle and buffalo meat using multiplex polymerase chain reaction. Buffalo Bulletin, 42(1), 57–66. https://doi.org/10.56825/bufbu.2023.4213617
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