Neoplastic conditions of ovaries in buffaloes- A abattoir based study


  • Tirumaran Srinivasan Department of Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Kurumbapet, India
  • Avinash Warundeo Lakkawar Department of Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Kurumbapet, India
  • Raja Kumar Department of Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Kurumbapet, India
  • Madhavan Gopalakrishnan Nair Department of Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Kurumbapet, India
  • Khub Chand Varshney Department of Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Kurumbapet, India



Bubalus bubalis, buffaloes , epithelial, mesenchymal origin, sex cord tumours, ovary


An abattoir-based study was carried out to assess the neoplasms affecting the ovaries of buffaloes. A total of 200 pairs of ovaries from 3 to 12 years old she buffaloes carcasses were examined and collected for routine histopathological studies. Ovarian neoplasms were recorded in 12 animals accounting for 6% and in all the cases the involvement was unilateral. Epithelial tumours were recorded in 6 cases (three each in right and left). Histologically, all the epithelial tumours were adenomas and were further sub-classified as simple adenoma (3 cases), fibro-adenoma (2 cases) and a solitary case of papillary cystadenoma. Sex cord-stromal tumors were recorded in 4 cases that included a solitary case of granulosa cell tumour in the right ovary and luteoma in 3 cases (one in right and two in left). Among the tumours of mesenchymal origin, haemangioma was observed in 2 cases involving each of left and right ovary.


Download data is not yet available.


Metrics Loading ...


Ali, A., A.K. Abdel-Razek, R. Derar, H.A. Abdel-Rheem and S.H. Shehata. 2009. Forms of reproductive disorders in cattle and buffaloes in Middle Egypt. Reprod. Domest. Anim., 44(4): 580-586. DOI: 10.1111/j.1439-0531.2007.01022.x

Ananda Raja, R. and Ch. Srilatha. 2007. Cystic ovarian degeneration in buffaloes. Indian J. Vet. Pathol., 31(2): 147-150. Available on:

Azab, A.M.S.M. 2005. Morphopathological studies on some affections in female genital tract in buffaloes. Ph.D. Thesis, Cairo University, Cairo, Egypt.

Azawi, O.I., A.J. Ali and E.M. Lazim. 2008. Pathological and anatomical abnormalities affecting buffalo cows reproductive tracts in Mosul. Iraqi Journal of Veterinary Sciences, 22(2): 59-67. DOI: 10.33899/ijvs.2008.5715

Banu, T.A., M. Shamsuddin and J. Bhattarcharjee. 2012. Milk progesterone enzyme-linked immunosorbent assay as a tool to investigate ovarian cyclicity of water buffaloes in relation to body condition score and milk production. Acta Vet. Scand., 54(1): 30-37. DOI: 10.1186/1751-0147-54-30

Benoit, J.M., R.C. Lefebvre, P.Y. Mulon, I. Raggio and M. Doré. 2005. Ovarian vascular hamartoma in a cow. Canadian Vet. J., 46(11): 1026‐1028.

Brackenbury, B. and M. Rebelatto. 2002. Bovine Granulosa Cell Tumors. Spring Newsletter Purdue University, West Lafayette, USA.

Carlton, W., M.D. McGavin and R.G. Thomson. 1995. Thomson's Special Veterinary Pathology, 2nd ed. Mosby, St. Louis, London, UK.

Damodaran, S. 1956. Studies on genital pathology of the female buffaloes (Bos bubalis). M.Sc. Thesis, Madras Veterinary College, Tamil Nadu, India.

Deeb, S., N.M. Safwat, M.B. El-Begawey and K.A. El-Nesr. 2018. A new classification of ovarian granulosa cell tumor based on histopathology in Egyptian cows and buffaloes. Journal of Veterinary Pathology Research, 2(1): 1-9. Available on:

Dobson, H. and M. Kamonpatana. 1986. A review of female cattle reproduction with special reference to a comparison between buffaloes, cows and Zebu. J. Reprod. Fertil., 77(1): 1-36. DOI: 10.1530/jrf.0.0770001

Dwivedi, J.N. and C.M. Singh. 1970. Studies on the pathology of female reproductive organs of Indian buffalo. Occurrence of rete ovarii and cystadenoma. Indian Vet. J., 47(2): 115-118.

Dwivedi, J.N. 1969. Hemangioma in lung of buffalo. Indian Vet. J., 46(4): 288-289.

Dwivedi, J.N. and C.M. Singh. 1971. Studies on the pathology of female reproductive organs of Indian buffaloes. 1. Ovarian abnormalities. Indian J. Anim. Health, 41: 10-27.

Dwivedi, J.N. and C.M. Singh. 1975. Studies on the folliculoids in ovaries of Indian buffalo. Indian J. Anim. Sci., 45: 15-20.

El-Nesr, K.A., H.H. Kamel and A.H. Abd-El-Rahman. 2006. Bovine ovarian granulose cell tumors: Pathological and clinicopathological studies. Egyptain Journal of Comparative Pathology and Clinical Pathology, 19: 228-245.

Ganorkar, A.G. and D.L. Paikne. 1994. Diseases of ovary in slaughtered buffaloes. Buffalo Bull., 13(4): 82-85. Available on:

Gerhenson, D.M. and J.T. Wharton. 2013. Sex-cord-stromal tumors of the ovary: Granulosa-stromal cell tumors. In Goff, B. and R.L. Garcia (eds.) Gynecologic Oncology. Available on:

Honparkhe, M., V.K. Gandotra and A.S. Nanda. 2004. Ultrasonographic measurements in comparison with the rectal palpation and echotexture of reproductive organs of buffaloes (Bubalus bubalis) during different stages of the estrous cycle. Asian Austral. J. Anim., 17(7): 919-923. DOI: 10.5713/ajas.2004.919

Hunt, J.T.C., D. Ronald and K.W. Norval. 1996. Veterinary Pathology, 6th ed. Clinical Chemistry, Academic Press, New York, USA.

Jainudeen, M.R. 1986. Reproduction in water buffalo. In Morrow, D.A. (edn.) Current Therapy in Theriogenology, 1st ed. W.B. Saunders Company, Philadelphia, USA. p. 43-449.

Jeevanajyothi, B.S., K.S. Rao and K.G.S. Raja. 2003. Reproductive disorders in buffaloes of Rangareddy district of Andhra Pradesh. Indian J. Anim. Res., 37(2): 107-109.

Jones, T.C., R.J. Hunt and N.W. King. 1996. Veterinary Pathology, 6th ed. Williams and Wilkins, Baltimore, London, UK.

Jubb, K.V.F., P.C. Kennedy and N. Palmer. 1993. Pathology of Domestic Animals, 4th ed. Elsevier Science, California, USA.

Khan, A., M.Z. Khan and M. Ahmad. 1989. Pathological studies on reproductive organs of the Nili-Ravi buffalo. Buffalo J., 5(2): 197-203.

Khan, M.Z. 1991. Prevalence and pathology of disease conditions of ovaries of Nili Ravi buffaloes. Buffalo J., 7(2): 215-223.

Kitano, Y., I. Iwabuchi, T. Keruma, Y. Ishikawa and K. Kadota. 1996. Ovarian sex cord‐stromal tumours in cattle, with particular reference to the lamination of basal laminae. Zentralbl Veterinarmed A, 43(9): 531-541. DOI: 10.1111/j.1439-0442.1996.tb00485.x

Kumar, N. and B. Singh. 1984. Some neoplasms involving female genitalia of buffaloes. Indian Vet. J., 61(3): 185-187.

Kumar, N. and B. Singh. 1985. Pathology of inflammatory conditions of female genitalia in buffaloes. Indian Vet. J., 62: 365-368.

Kumaresan, A. and M.R. Ansari. 2002. Incidence of reproductive disorders of female buffaloes. An abattoir survey. Indian J. Vet. Med., 26: 141-143.

Lee, C.G. and P.W. Ladds. 1976. Vascular hamartoma of the ovary in a cow. Aust. Vet. J., 52: 236.

Luktuke, S.N., A.R. Bhatacharya and S.K. Singh. 1973. Studies on aberrations in functional activity of the ovaries in buffaloes. Indian Vet. J., 50(9): 876-881.

Luna, L.G. 1968. Manual of Histological Staining Methods of the Armed Forces Institute of Pathology, McGraw-Hill, New York, USA.

Mac Lachlan, N.J. 1987. Ovarian disorders in domestic animals. Environ. Health Persp., 73: 27-33. DOI: 10.1289/ehp.877327

McEntee, K. 1990. Ovarian neoplasms. Reproductive Pathology of Domestic Mammals, Academic Press, San Diego, New York, USA. p. 69-93.

McEntee, K. 1990. Reproductive Pathology of Domestic Animals, 4th ed. Academic Press, Inc., New York, USA.

Meuten, D.J. 2017. Tumors in Domestic Animals, 5th ed. John Wiley and Sons, Iowa, USA.

Mittal, D., U.K. Garg and G.P. Jatav. 2010. Prevalence of different pathological affections of ovaries in buffaloes (Bubalus bubalis) in Malwa region of Madhya Pradesh. Buffalo Bull., 29(1): 39-42. Available on:

Modi, L.C., P.A. Patel and S.P. Patel. 2011. Prevalence of reproductive problems in buffalo in Mehsana milk-shed area of Gujarat. International Journal for Agro Veterinary and Medical Sciences, 5(4): 424-428. DOI: 10.5455/ijavms.20110801094631

Moulton, J.E. 1990. Tumours in Domestic Animals, 3rd ed. California University of California, California, USA.

Narnaware, S.D., K. Sirothia and A.G. Bhandarkar. 2007. Ovarian tumours in buffaloes (Bubalus bubalis). Indian Journal of Veterinary Pathology, 31(1): 60-61. Available on:

Narsinha Rao, A.V. and O. Sreemannarayana. 1982. Clinical analysis of reproductive failure among female buffaloes (Bubalus bubalis) under village management in Andhra Pradesh. Theriogenology, 18(4): 403-411. DOI: 10.1016/0093-691x(82)90162-5

Nasrin, A., A. Baharak and K. Reza. 2012. Concurrent cystic endometrial hyperplasia, ovarian luteoma and biliary cyst adenoma in an aged rabbit (Oryctolagus cuniculus): Case report and literature review. Asian Pacific Journal of Tropical Biomedicine, 2(3): S1975-S1978. DOI: 10.1016/S2221-1691(12)60527-9

Pande, M., G.K. Das, R. Singh, S.S. Ramteke, F.A. Khan, H. Kumar, J.S. Rajoriya and N. Srivastava. 2016. Ovarian Teratoma in a water buffalo (Bubalus Bubalis). Buffalo Bull., 35(4): 557-562. Available on:

Patel, S.B., K.K. Hadiya and D.B. Chavan. 2007. Incidence of reproductive disorders in cattle and buffaloes in Kaira district of Gujarat. Indian Journal of Field Veterinarians, 3: 15-17.

Potekar, A.M., B.R. Deshpande and D.R. Pargaonkar. 1982. Note on ovarian abnormalities in Indian buffaloes. Indian J. Anim. Sci., 52(10): 951-953.

Purohit, G.N. 2014. Ovarian and oviductal pathologies in the buffalo: Occurrence, diagnostic and therapeutic approaches. Asian Pacific Journal of Reproduction, 3(2): 156-168. DOI: 10.1016/S2305-0500(14)60020-8

Qureshi, S., I.M. Khan and R.A. Chaudhry. 1992. Comparitive efficiency of rectal palpation and milk progesterone profiles in diagnosis of ovarian contents in buffaloes. Pakistan Journal of Agricultural Research, 13(2): 196-200.

Radostits, O.M., C.C. Gay, D.C. Blood and K.W. Hinchcliff. 2000. Veterinary Medicine-A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses, 9th ed. W.B. Saunders Company Ltd., London, UK. p. 1684-1688.

Raja, A.R. and C. Srilatha. 2008. Incidence of pathological conditions of ovaries of buffaloes. Indian Vet. J., 85: 997-998.

Raju, K.G.S., K.V. Naidu and K.S. Rao. 2007. Incidence of reproductive disorders in buffaloes under field conditions of Andhra Pradesh. Indian Journal of Animal Reproduction, 28(1): 46-48.

Saxena, G., S. Rani and H. Danodia. 2006. Pathological conditions in genital tract of female buffaloes (Bubalus bubalis). Pak. Vet. J., 26(2): 91-93. Available on:

Sharifuddin, A.W. 1983. Assessment of ovarian function in the swamp buffalo (Bubalus bubalis). M.Sc. Thesis, University of Pertanian, Serdang, Selangor Darul Ehsan, Malaysia.

Sharma, V.K., R.C. Gupta, S.K. Mishra, N.K. Khurana and S.K. Khar. 1993. An abattoir study of lesions in buffalo genitalia. Indian Vet. J., 70: 1165-1167.

Talerman, A. and R. Vang. 2014. Non specific tumors of the ovary, including Mesenchymal tumors. Balusteins Pathology of the Genital Tract. Springer Verlag. New York, USA.

Terzano, G.M. 2012. Ultrasonography and reproduction in buffalo. Journal of Buffalo Science, 1: 163-173. Available on:

Yener, Z., F. Karaca and M. Alan. 2004. Serous papillary cystadenoma in the ovary of a cow. Aust. Vet. J., 82(12): 781-783. DOI: 10.1111/j.1751-0813.2004.tb13250.x

Zinnebauer, H. 1961. Ein Besonders grosser ovarian tumor. Wein Tierarztl Monasschr, 48: 944-947.

Granulosa cell tumour showing neoplastic cells arranged in tubular pattern. H & E x 200




How to Cite

Srinivasan, T., Lakkawar, A. W., Kumar, R., Nair, M. G., & Varshney, K. C. (2024). Neoplastic conditions of ovaries in buffaloes- A abattoir based study. Buffalo Bulletin, 43(1), 55–67.



Original Article