Development and application of a loop-mediated isothermal amplification method for rapid detection of Pasteurella multocida

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Sivaramakrishna Akondi Anil Kumar Arora Narinder Singh Sharma

Abstract

Pasteurella multocida is an important pathogen affecting livestock and poultry causing significant economic losses. A loop-mediated isothermal amplification (LAMP) assay using four primers targeting a conserved region of the kmt1 gene was standardised to diagnose Pasteurella multocida. The test was carried out at 64oC for 45 minutes, the LAMP products could be visually confirmed using fluorescent dye SYBR Green I as detection reagent both with naked eye as well as under UV-illumination. The sensitivity of the developed LAMP assay was 104 fold higher than PCR. Furthermore, no cross-reactivity was found with the other tested bacteria. The developed LAMP assay allows easy, rapid, accurate and sensitive detection of Pasteurella multocida.

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How to Cite
AKONDI, Sivaramakrishna; ARORA, Anil Kumar; SHARMA, Narinder Singh. Development and application of a loop-mediated isothermal amplification method for rapid detection of Pasteurella multocida. Buffalo Bulletin, [S.l.], v. 41, n. 2, p. 318-327, june 2022. ISSN 2539-5696. Available at: <https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/3763>. Date accessed: 16 aug. 2022.
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Original Article

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