Identification of Chlamydia abortus and Chlamydia pecorum in water buffaloes (Bubalus bubalis) and cows cohabitating the same herd

Magdalena Limón-González; Rigoberto Hernández-Castro; Gabriela Palomares Reséndiz; Enrique Herrera López; Efrén Díaz Aparicio

doi:10.56825/bufbu.2023.4243989

Authors

Magdalena Limón-González Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, Ciudad de México, Mexico
Rigoberto Hernández-Castro Departamento de Ecología de Agentes Patógenos. Hospital General Manuel Gea González, Ciudad de México, Mexico
Gabriela Palomares Reséndiz CENID Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Ciudad de México, Mexico
Enrique Herrera López CENID Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Ciudad de México, Mexico
Efrén Díaz Aparicio CENID Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Ciudad de México, Mexico

DOI:

https://doi.org/10.56825/bufbu.2023.4243989

Keywords:

Bubalis bubalis, water buffaloes, cattle, Chlamydia abortus, Chlamydia pecorum, Mexico

Abstract

The aim of this study was to determine the presence of Chlamydia spp. in a dual-purpose zebu and water buffalo-mixed herd cohabitating in a ranch located in southern Mexico. The zootechnical purpose of this herd is milk and cheese production. A total of 52 vaginal exudate samples were obtained from clinically healthy water buffaloes, less than one month after parturition, and two vaginal exudate samples were taken from cows that had recently aborted. L929 cells were used for bacterial isolation. Two of these cultures were infected, confirmed by direct immunofluorescence. Total DNA was extracted for analysis with two types of real-time PCR, a Chlamydiaceae-specific real-time PCR and another species-specific real-time PCR for C. abortus, C. psittaci and C. pecorum. Two Chlamydia isolates were obtained, one from a water buffalo positive to C. abortus and C. pecorum, the other from a cow positive to C. abortus. This is the first report of the presence of C. abortus and C. pecorum in water buffaloes in Mexico. C. abortus was also detected in one cow living in the same herd as the water buffaloes.

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