Assaying the concentration of immunoglobulin G in colostrum from females postpartum and serum from neonatal calves of buffaloes (Bubalus bubalis)


  • Swati Agrawal Division of Animal Genetics, Indian Council of Agricultural Research, Indian Veterinary Research Institute, Uttar Pradesh, India
  • Tarun Kumar Division of Pharmacology and Toxicology, Indian Council of Agricultural Research, Indian Veterinary Research Institute, Uttar Pradesh, India
  • Rajni Chaudhary Indian Council of Agricultural Research, North Temperate Research Station, Himachal Pradesh, India
  • Anitta Pulikan Lionel Division of Animal Genetics, Indian Council of Agricultural Research, Indian Veterinary Research Institute, Uttar Pradesh, India
  • Subodh Kumar Division of Animal Genetics, Indian Council of Agricultural Research, Indian Veterinary Research Institute, Uttar Pradesh, India



Bubalus bubalis, buffaloes, colostrum, failure of passive transfer, immunoglobulin G, serum


Neonatal ruminants are born without any humoral immunity due to lack of placental transfer of immunoglobulins during gestation. This predisposes the newborn buffalo calves to a high incidence of morbidity and mortality on exposure to infectious agents. Colostrum is the first milk produced by the females after calving and is a rich source of immunoglobulins, especially immunoglobulin G (IgG). The immunocompetence of the neonates can be boosted by feeding them sufficient amount of good quality colostrum within a few hours after birth. Optimal colostrum management at a farm not only reduces the occurrence of diseases among the younger stock but also enhances their growth performance and productivity once they are adults. In the present study, eighty animals at a Murrah buffalo farm were screened for the concentration of IgG in the colostrum collected from recently parturated females and in the serum collected from their calves within 6 to 12 h of colostrum consumption to determine the status of transfer of passive immunity. Indirect ELISA was used to estimate the IgG levels. The overall mean (range) of colostral and serum IgG concentration was found to be 50.44±3.36 (12.71 to 227.78) and 10.85±0.62 (0.25 to 19.88) mg/ml, respectively for all the 80 animals. Routine screening of buffaloes, in a similar way, will help to reduce calf deaths due to immunodeficiency.


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Author Biographies

Swati Agrawal, Division of Animal Genetics, Indian Council of Agricultural Research, Indian Veterinary Research Institute, Uttar Pradesh, India,

Tarun Kumar, Division of Pharmacology and Toxicology, Indian Council of Agricultural Research, Indian Veterinary Research Institute, Uttar Pradesh, India

Tarun Kumar

Rajni Chaudhary, Indian Council of Agricultural Research, North Temperate Research Station, Himachal Pradesh, India

Rajni Chaudhary

Anitta Pulikan Lionel, Division of Animal Genetics, Indian Council of Agricultural Research, Indian Veterinary Research Institute, Uttar Pradesh, India

Subodh Kumar, Division of Animal Genetics, Indian Council of Agricultural Research, Indian Veterinary Research Institute, Uttar Pradesh, India

Subodh Kumar*


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How to Cite

Agrawal, S., Kumar, T., Chaudhary, R., Lionel, A. P., & Kumar, S. (2022). Assaying the concentration of immunoglobulin G in colostrum from females postpartum and serum from neonatal calves of buffaloes (Bubalus bubalis). Buffalo Bulletin, 41(3), 493–509.



Original Article