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Tropical theileriosis poses major threat for buffaloes causing significant economical loss to livestock farmers. Early detection and prompt treatment helps to minimise mortality and economical loss. This study was conducted on 79 female buffaloes presented with the signs suggestive of theileriosis. Evaluation of PCR for detection and buparvaquone for efficacy was undertaken. Overall prevalence of T. annulata infection recorded was 22.78 % by PCR. Adult buffaloes showed higher prevalence (13.92 %) compared to young buffaloes (8.86 %). Blood smear examination revealed 38.89 % sensitivity in detection of Theileria piroplasms. Haematological observations showed significant decreased values of Hb, TEC, PCV and TLC. Neutropenia, monocytopenia, eosinopenia and lymphocytosis were recorded. Buparvaquone was 100 % effective in complete elimination of T. annulata in infected buffaloes.