Effects of age on the haematological and biochemical parameters in Romanian buffalo
DOI:
https://doi.org/10.56825/bufbu.2024.4335224Keywords:
Bubalus bubalis, buffaloes, animal physiology, biochemical profile, haematological profile, Romanian buffaloAbstract
The aim of this study was to investigate the changes of hematic constants related to age in Romanian buffalo, in order to establish references values based on age of the animals. A number of 45 Romanian buffalo animals, clinically healthy, were divided into 3 groups (n=15/group) according to their age, as follows: Group T1 = un-weaned buffalo calves of 1 to 3 months of age, Group T2 = young buffalo heifers of 6 to 12 months, and Group T3 = buffalo adult cows of 3 to 5 years of age. Different hematological parameters and biochemical parameters were investigated in the three groups. Generally, a significant effect of age on both hematological and biochemical indices studied was observed (P<0.05). The concentration of total cholesterol and the concentration of inorganic phosphorous were not influenced by age of the animals (P>0.05). Significant positive correlations were found between total protein and age of the animals (P=0.0019), while significant negative correlations were observed between hemoglobin (P=0.0227), white blood cells count (P=0.0352), lymphocytes (P=0.0002), platelet (P=0.0022), total cholesterol (P=0.1201), calcium (P=0.0077) and age of the animals. The results of the current pilot study could prove useful in establishing a baseline and health alarm thresholds for hematic parameters in water-buffalo, based on age of the animals and physiological status.
Downloads
Metrics
References
Borjesson, D.L., M.M. Christopher and W.M. Boyce. 2009. Biochemical and hematologic reference intervals for free-ranging desert bighorn sheep. J. Wildlife Dis., 36(2): 294-300. DOI: 10.7589/0090-3558-36.2.294
Brockus, C.W. 2011. Erythrocytes, 5th ed. In Latimer, K.S. (edn.) Duncan and Prasse’s Veterinary Laboratory Medicine: Clinical Pathology, Wiley, Chichester, UK. 3-44p.
Brun-Hansen, H.C., A.H. Kampen and A. Lund. 2006. Hematologic values in calves during the first 6 months of life. Vet. Clin. Path., 35(2): 182-187. DOI: 10.1111/j.1939-165x.2006.tb00111.x
Campanile, G., W. Taccone, M. Palladino, C.D. Meo and R.D. Palo. 1993. Influence of age and month of sampling on serum enzymes and electrophoretic picture in buffalo calves in the lactation phase. In Note I. Proceedings III FeMeSPRum Conference, Teramo, Italy.
Couch, C.E., M.A. Movius, A.E. Jolles, M.E. Gorman, J.D. Rigas and B.R. Beechler. 2017. Serum biochemistry panels in African buffalo: Defining reference intervals and assessing variability across season, age and sex, PLoS One, 12(5): 1-15. DOI: 10.1371/journal.pone.0176830
Devi, R. and M.P. Kumar. 2012. Effect of ageing and sex on the caeruloplasmin (Cp) and the plasma protein levels. Journal of Clinical and Diagnostic Research, 6(4): 577-580. DOI: 10.7860/JCDR/2012/.2118
Dhillon, K.S., C.S. Randhawa, K. Gupta, R.S. Singh and S. Chhabra. 2020. Reference values for haematological and biochemical profile in adult Indian buffaloes. Buffalo Bull., 39(2): 145-154. Available on: https://kukrdb.lib.ku.ac.th/journal/BuffaloBulletin/search_detail/result/400635
Directive. 2010. Directive 2010/63/EU of the European Parliament and of the Council on the Protection of Animals Used for Scientific Purposes. Official Journal of the European Union, 276: 33-79. European Union, Bruxelles, Belgique. Available on: https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri= OJ:L:2010:276:0033:0079:en:PDFFAOS
TAT. 2023. Gateway to Dairy Production and Products, Food and Agriculture Organization of the United Nations, Rome, Italy. Available on: https://www.fao. org/dairy-production-products/production/ dairy-animals/buffaloes/en/
Haque, N., A. Ludri, H. Ashutosh, A. Shahed and M. Ashutosh. 2013. Impact on hematological parameters in young and adult Murrah buffaloes exposed to acute heat stress. Buffalo Bull., 32(4): 321-326. Available on: https://kukrdb.lib.ku.ac.th/journal/BuffaloBulletin/search_detail/result/286443
Husakova, T., L. Pavlata, A. Pechova, K. Hauptmanova, E. Pitropovska and L. Tichy. 2014. Reference values for biochemical parameters in blood serum of young and adult alpacas (Vicugna Pacos). Animal, 8(9): 1448-455. DOI: 10.1017/S1751731114001256
Islam, S., J. Ferdous, M.K. Rahman, S. Akter, M.M. Hassan and A. Islam. 2019. Reference values for hematological and serum biochemical parameters of dromedary camel (Camelus dromedarius) in sub-tropical climate of Bangladesh. Advanced Animal Veterinary Sciences, 7(4): 232-237. Available on: https://nexusacademicpublishers.com/uploads/files/AAVS_7_4_232-237.pdf
Jaramillo, I., P. Agudelo-Florez, J. Tobon and J.R. Buitrago. 2022. Hematological values in water buffaloes, variations with age, sex, and Trypanosoma spp. natural infection. Research Square, DOI: 10.21203/rs.3.rs-2234794/v1
Khan, I.S., C. Singh, S. Tejinder and K. Dua. 2018. Age related changes in blood biochemical and hematological profile of buffalo in calves. J. Vet. Sci. Technol., 9(1): 1-4. DOI: 10.4172/2157-7579.1000512
Khan, A., K. Singh, S. Jaiswal, M. Raza, R.S. Jasrotia, A. Kumar, A.K.S. Gurjar, J. Kumari, V. Nayan, M.A. Iquebal, U.B. Angadi, A. Rai, T.K. Datta and D. Kumar. 2022. Whole-genome-based web genomic resource for water buffalo (Bubalus bubalis). Frontiers in Genetics, 11. DOI: 10.3389/fgene.2022.809741
Klinkon, M. and J. Jezek. 2012. Values of blood variables in calves. In Perez-Marin, C.C. (edn.) A Bird’s-Eye View of Veterinary Medicine, 301-320. InTech, Croatia, DOI: 10.5772/32100
Kuralkar, P., S.A. Dhenge and S.V. Kuralkar. 2018. Age related changes in haematology and plasma biochemical profile in Purnathadi buffalo, Buffalo Bull., 37(3): 291-296. Available on: https://kukrdb.lib.ku.ac.th/journal/BuffaloBulletin/search_detail/result/383060
Law 43/2014. Low 43 of the Romanian Parliament of 06 May 2014 on the Handling and Protection of Animals Used for Scientific Purposes, Official Monitor of Romania, no.326/Part I: 2-29. Available on: https://monitoruloficial.ro/Monitorul-Oficial--PI--326--2014.html
Li, W., D.M. Bickhart, L. Ramunno, D. Iamartino, J.L. Williams and G.E. Liu. 2019. Comparative sequence alignment reveals River buffalo genomic structural differences compared with cattle. Genomics, 111(3): 418-425. DOI: 10.1016/j.ygeno.2018.02.018
Miller, M., M. Weber, E.V. Valdes, D. Neiffer, D. Fontenot, G. Fleming and M. Stetter. 2010. Changes in serum calcium, phosphorus, and magnesium levels in captive ruminants affected by diet manipulation. J. Zoo. Wildlife Med., 41(3): 404-408. DOI: 10.1638/2009-0001.1
Mohan, N.H., N. Debasish, J.Y. Waghaye and H.N. Singh. 2009. Age-related haematological changes in Murrah buffalo (Bubalus bubalis) calves. Indian J. Anim. Sci., 79(4): 369-371.
Mohri, M., K. Sharifi and S. Eidi. 2007. Hematology and serum biochemistry of Holstein dairy calves: Age related changes and comparison with blood composition in adults. Res. Vet. Sci., 83(1): 30-39. DOI: 10.1016/j.rvsc.2006.10.017
Patel, M.D., A. Lateef, H. Das, A.S. Patel, A.G. Patel and A.B. Joshi. 2016. Effect of age, sex and physiological stages on hematological indices of Banni buffalo (Bubalus bubalis). Vet. World, 9(1): 38-42. DOI: 10.14202/vetworld.2016.38-42.
Roland, L., M. Drillich and M. Iwersen. 2014. Hematology as a diagnostic tool in bovine medicine. J. Vet. Diagn. Invest., 26(5): 592-598. DOI: 10.1177/1040638714546490
Sharma, K., M. Sharma, S. Mishra, O.P. Dinani and S. Nety. 2019. Comparative evaluation of hemato biochemical profile in healthy and parasitic infected buffalo calves. Indian Journal of Pure and Applied Biosciences, 7(2): 459-462. DOI: 10.18782/2320-7051.7330
Siddhapara, B.S., V.K. Singh, S.S. Chaudhary, G. Puri, A.B. Joshi, T.D. Manat and P.G. Rathod. 2022. Clinico-hematological profile of diarrheic Surti buffalo calves. Pharma Innovation Journal, 11(10): 2363-2367. Available on: https://www.thepharmajournal.com/archives/2022/vol11issue10S/PartAB/S-11-10-232-176.pdf