Evaluation of milk fat as an alternative source of RNA from buffalo milk

Authors

  • Anju Sharma Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India
  • Yashila Girdhar Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India
  • Mandeep Kaur Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India
  • Sonika Ahlawat Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India
  • Ramesh Kumar Vijh Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India
  • Reena Arora Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Keywords:

buffalo, fat, milk, qPCR, RNA

Abstract

The study reports optimization of RNA extraction method from buffalo milk fat, to be used for gene expression analysis. Higher yields were obtained from buffalo milk fat as compared to skim milk, with manual method as well as commercial kits. Quality and integrity of the samples from both methods was comparable. Quantitative profiling of mammary gland specific genes from skim milk and fat derived RNA validated the efficiency of MFG as an alternate source of total RNA. This study confirmed that the RNA obtained from buffalo MFG was suitable for studies of mammary genes expression.  

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Author Biographies

Anju Sharma, Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Yashila Girdhar, Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Mandeep Kaur, Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Sonika Ahlawat, Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Ramesh Kumar Vijh, Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Reena Arora, Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Haryana, India

Buffalo Bulletin Vol.37 No.2

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Published

2018-06-30

How to Cite

Sharma, A., Girdhar, Y., Kaur, M., Ahlawat, S., Vijh, R. K., & Arora, R. (2018). Evaluation of milk fat as an alternative source of RNA from buffalo milk. Buffalo Bulletin, 37(2), 151–156. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/553

Issue

Vol. 37 No. 2 (2018): April-June

Section

Original Article