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The present study on incidence of hydatid disease in buffaloes was conducted to evaluate the incidence of hydatid cyst grossly as well as by means of polymerase chain reaction in buffaloes. Results indicated an overall incidence of 12% of hydatid disease in buffaloes in Akola district of Maharashtra. Liver (75%) showed the most predominant site as compared to lung. All most all the cysts were of small size (87.50%) having below 5 cm in diameter and are single in organ. Most of the hydatid cysts in liver (62.50%) did not revealed protoscolices hence considered as sterile. Each PCR isolate obtained from individual hydatdi cyst were further showed 434 base pair fragment of the mitochondrial cytochrome oxidase -1 gene on 1.5% agarose gel electrophoresis and confirmed the hydatid disease.
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