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The present study was conducted to investigate the normal somatic cell count (SCC), associated factors in hygienic management and to define subclinical mastitis in buffaloes in Puducherry. Samples were collected from 50 clinically normal buffaloes stationed at different farms of Puducherry region. Somatic cell count was measured for all the milk samples using Newman-Lampert staining technique. The upper limit of SCC was determined as ≥2, 00,000/ml of milk based on the mean ± 2SD of a total SCC. California mastitis test (CMT) was conducted for all the animals. In the present study subclinical mastitis was diagnosed on the basis of samples with SCCs ≥ 2, 00,000/ml. In the present study 96% of the milk samples were negative for California mastitis test (CMT). The SCC of CMT positive quarter was significantly higher (P<0.01) than CMT negative quarters. Subclinical mastitis was found only in three buffaloes in the present study. Somatic cell counts were significantly higher in the buffaloes with subclinical mastitis. Regarding the hygiene, all the farms had poor management and improper housing. Proper education on hygienic management is need of the hour for buffalo owners of Puducherry region.
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