IMPACT OF CRYOPRESERVATION ON THE STRUCTURAL DIMENSIONS  OF MURRAH BULL SPERMATOZOA

Chetna Gangwar; Atul Saxena; Akhil Patel; Shriprakash Singh; Sarvajeet Yadav; Vijay Singh; Vipin Singh

doi:10.56825/bufbu.2026.4526476

Authors

Chetna Gangwar Department of Veterinary Clinical Complex, College of Veterinary Science and Animal Husbandry, Uttar Pradesh Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Uttar Pradesh, India
Atul Saxena Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Uttar Pradesh Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Uttar Pradesh, India
Akhil Patel Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Sardar Vallabhbahi Patel University of Agriculture and Technology, Uttar Pradesh, India
Shriprakash Singh Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry, Uttar Pradesh Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Uttar Pradesh, India
Sarvajeet Yadav Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, Uttar Pradesh Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Uttar Pradesh, India
Vijay Singh Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Sardar Vallabhbahi Patel University of Agriculture and Technology, Uttar Pradesh, India
Vipin Singh Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Uttar Pradesh Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Uttar Pradesh, India

DOI:

https://doi.org/10.56825/bufbu.2026.4526476

Keywords:

Bubalus bubalis, buffaloes, cryopreservation, fertility, Murrah bull, morphometry, sperm

Abstract

The present study was conducted to evaluate the effect of cryopreservation on sperm morphometry of Murrah bull spermatozoa. A total of 16 ejaculates were collected from Murrah bulls for this study. Semen was collected with Artificial Vagina method at twice a week interval. Semen was evaluated for normal sperm parameters and sperm morphometry was done for normal samples of fresh semen. Semen was extended in glycerolated egg yolk tris extender and equilibrated for 4 h at 5^oC, followed by cryopreservation in automatic biological freezer. Semen samples were evaluated after thawing at 37^oC for 45 seconds for normal parameters and sperm morphometry. Findings of the present study revealed that there was significant difference was observed in sperm head morphometry after cryopreservation. Therefore, the study was concluded with that the cryopreservation alters the sperm architecture which might be responsible for reduced fertility after cryopreservation.

Downloads

Download data is not yet available.

References

Aggarwal, R.A.K., S.P.S. Ahlawat, Y. Kumar, P.S. Panwar, K. Singh and M. Bhargava. 2007. Biometry of frozen-thawed sperm from eight breeds of Indian buffaloes (Bubalus bubalis). Theriogenology, 68(4): 682-686. DOI: 10.1016/j.theriogenology.2007.03.028 DOI: https://doi.org/10.1016/j.theriogenology.2007.03.028

Ahmad, N. 1994. Clinical and experimental studies of reproductive functions in the ram and male goat with special reference to the use of diagnostic ultrasound. Ph.D. Thesis, Department of Large Animal Medicine, and Surgery, Royal Veterinary College, University of London, London, England.

Bane, A. 1954. Studies on monozygous cattle twins. 15. Sexual functions of bulls in relation to herediry, rearing intensity and somatic conditions. Acta Agric. Scand., 4: 95-208. DOI: 10.1080/00015125409439936 DOI: https://doi.org/10.1080/00015125409439936

Biswajit, R. 2014. A comparative study on sperm morphometry of crossbred and Murrah buffalo bulls, International Journal of Agricultural Sciences and Veterinary Medicine, 2(3): 149-155.

Campbell, R.G., J.L. Hancock and L. Rothschild. Counting live and dead bull spermatozoa. Journal of Experimental Biology, 30(1): 44-49. DOI: 10.1242/jeb.30.1.44 DOI: https://doi.org/10.1242/jeb.30.1.44

Casey, P.J., C.G. Gravance, R.O. Davis, D.D. Chabot and I.K.M. Liu. 1997. Morphometric differences in sperm head dimensions of fertile and subfertile stallions. Theriogenology, 47(2): 575-582. DOI: 10.1016/S0093-691X(97)00015-0 DOI: https://doi.org/10.1016/S0093-691X(97)00015-0

Esteso, M.C., A.J. Soler, M.R. Fernandez-Santos, A.A. Quintero-Moreno and J.J. Garde. 2006. Functional significance of the sperm head morphometric size and shape for determining freezability in Iberian Red Deer (Cervus elaphus hispanicus) epididymal sperm samples. J. Androl., 27(5): 662-670. DOI: 10.2164/jandrol.106.000489 DOI: https://doi.org/10.2164/jandrol.106.000489

Francisco, A., V.L. Garcia, G. Joaquin, M. Carmen and V.H. William. 2016. Importance of sperm morphology during sperm transport and fertilization in mammals. Asian J. Androl., 18(6): 844-850. DOI: 10.4103/1008-682X.186880 DOI: https://doi.org/10.4103/1008-682X.186880

Gangwar, C., R. Ranjan, S.D. Kharche, R. Pourouchottamane and B. Rai. 2023. Success of artificial insemination in goats: An overview. Indian Journal of Small Ruminants, 29(1): 1-10. DOI: 10.5958/0973-9718.2023.00017.X DOI: https://doi.org/10.5958/0973-9718.2023.00017.X

Gangwar, C., A. Saxena, S. Yadav, S.P. Singh, V. Singh and A. Patel. 2019. Cryopreservation induced sperm cryoinjuries in Hariana bull semen. International Journal of Livestock Research, 9(6): 136-147. DOI: 10.5455/ijlr.20190102061931 DOI: https://doi.org/10.5455/ijlr.20190102061931

Gravance, C.G., M.E. Casey and P.J. Casey. 2009. Pre-freeze bull sperm head morphometry related to post-thaw fertility. Anim. Reprod. Sci., 114(1-3): 81-88. DOI: 10.1016/j.anireprosci.2008.09.014 DOI: https://doi.org/10.1016/j.anireprosci.2008.09.014

Gravance, C.G., I.K.M. Liu, R.O. Davis, J.P. Hughes and P.J. Casey. 1996. Quantification of normal head morphometry of stallion spermatozoa. J. Reprod. Fertil., 108(1): 41-46. DOI: 10.1530/jrf.0.1080041 DOI: https://doi.org/10.1530/jrf.0.1080041

Gravance, C.G., R. Vishwanath, C. Pitt, D.L. Garner and P.J. Casey. 1998. Effects of cryopreservation on bull sperm head morphometry. J. Androl., 19: 704-709. DOI: 10.1002/j.1939-4640.1998.tb02079.x DOI: https://doi.org/10.1002/j.1939-4640.1998.tb02079.x

Hameed, S., S. Masood, H. Zaneb, M. Sarwar, M.Y. Khan, M. Avais and M.Ur.R. Khan. 2014. Dimensional characteristics of spermatozoa of Nili-Ravi buffalo bulls: Seasonal and climatic influences. Turk. J. Vet. Anim. Sci., 40(2): 207-213. DOI: 10.3906/vet-1508-56 DOI: https://doi.org/10.3906/vet-1508-56

Hancock, J.L. 1951. A staining technique for the study of temperature-shock in semen. Nature, 167. 323-326. DOI: 10.1038/167323b0 DOI: https://doi.org/10.1038/167323b0

Harapanhalli, M.D. and D.P. Mukherjee. 1973. Effect of different diluents on the cytomorphology of buffalo spermatozoa. Indian J. Anim. Prod., 4: 128-135.

Hirai, M., A. Boersma, A. Hoeflich, E. Wolf, J. Foil, R. Aumuller and R. Aumuller. 2001. Objectively measured sperm motility and sperm head morphometry in boars (Sus scrofa): Relation to fertility and seminal plasma growth factors. J. Androl., 22(1): 104-110. DOI: 10.1002/j.1939-4640.2001.tb02159.x DOI: https://doi.org/10.1002/j.1939-4640.2001.tb02159.x

Januskauskas, A., A. Johannisson and H. Rodrıguez-Martınez. 2003. Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility. Theriogenology, 60(4): 743-758. DOI: 10.1016/S0093-691X(03)00050-5 DOI: https://doi.org/10.1016/S0093-691X(03)00050-5

Mishra, G.K., R. Tiwari, S.U. Rehman, K.S. Rathore, R.B. Singh, S.K. Saxena and M.U. Siddiqui. 2012. Effect of seasons on semen production performance of Jersey bulls. Indian J. Dairy Sci., 65(6): 497-500.

Ostermeier, G.C., G.A. Sargeant, B.S. Yandell, D.P. Evenson and J.J. Parrish. 2001. Relationship of bull fertility to sperm nuclear shape. J. Androl., 22: 595-603. DOI: 10.1002/j.1939-4640.2001.tb02219.x DOI: https://doi.org/10.1002/j.1939-4640.2001.tb02219.x

Pena, F.J., F. Saravia, M.G. Herreros, I. Nunez, J.A. Tapia, A. Johannisson, M. Wallgren and H.R. Martínez. 2005. Identification of sperm morphometric subpopulations in two different portions of the boar ejaculate and its relation to postthaw quality. J. Androl., 26(6): 716-723. DOI: 10.2164/jandrol.05030 DOI: https://doi.org/10.2164/jandrol.05030

Salisbury, G.W., N.L. Van Denmark and J.R. Lodge. 1978. Physiology of Reproduction and Artificial Insemination of Cattle, Freeman, San Francisco, USA. p. 494-554.

Saravia, F., I.N. Martinez, J.M. Moran, C. Soler, A. Muriel, H.R. Martinez and F.J. Pena. 2007. Differences in boar sperm head shape and dimensions recorded by computer-assisted sperm morphometry are not related to chromatin integrity. Theriogenology, 68(2): 196-203. DOI: 10.1016/j.theriogenology.2007.04.052 DOI: https://doi.org/10.1016/j.theriogenology.2007.04.052

Tapaloaga, D. and P.R. Tapaloaga. 2015. Study regarding age-related morphometric features of buffalo sperm. Agriculture and Agricultural Science Procedia, 6: 272-276. DOI: 10.1016/j.aaspro.2015.08.071 DOI: https://doi.org/10.1016/j.aaspro.2015.08.071

Thurston, L.M., P.F. Watson, A.J. Mileham and W.V. Holt. 2001. Morphologically distinct sperm subpopulations defined by Fourier shape descriptors in fresh ejaculates correlate with variation in boar semen quality following cryopreservation. J. Androl., 22(3): 382-394. DOI: 10.1002/j.1939-4640.2001.tb02194.x DOI: https://doi.org/10.1002/j.1939-4640.2001.tb02194.x

Venkataswami, V. and A.R. Vedanayagam. 1962. Biometrics of spermatozoa of cattle and buffaloes. Indian Vet. J., 39: 287-291.

Watson, P.F. 1990. AI and the preservation of semen. In Lammingi, G.E. ed. Marshall’s Physiology of Reproduction, Churchill Livingstone, London, UK. p. 747-869.

Zhang, B.R., B. Larsson, N. Lundeheim and H.R. Martinez. 1998. Sperm characteristics and zona pellucida binding in relation to field fertility of frozen-thawed semen from dairy AI bulls. Int. J. Androl., 21(4): 207-216. DOI: 10.1046/j.1365-2605.1998.00114.x DOI: https://doi.org/10.1046/j.1365-2605.1998.00114.x