Isolation and partial characterization of Ruminococcus flavefaciens from the rumen of swamp buffalo

Authors

  • Phoompong Boonsaen Department of Animal Science, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Thailand
  • Somporn Poonko Department of Animal Science, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Thailand
  • Jeerachai Kanjanapruetipong Department of Animal Science, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Thailand
  • Pharima Phiriyangkul Division of Biochemistry, Department of Science, Faculty of Liberal Art and Science, Kasetsart University, Nakhon Pathom, Thailand
  • Suriya Sawanon Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand

Keywords:

Bubalus bubalis, Fibrolytic bacteria, buffaloes, Ruminococcus flavefaciens, fibrolytic bacteria, Swamp buffalo, non-fibrolytic bacteria, R. flavefaciens, rumen, swamp buffalo

Abstract

The ability to utilize low-quality roughage of swamp buffalo has been reported, thus, rumen of swamp buffalo might be a suitable source for isolation of potent fibrolytic bacteria. A total of 165 gram-negative cocci fibrolytic bacteria were screened from rumen contents of 4 swamp buffalo. Six isolates were identified as Ruminococcus flavefaciens and phylogenetically grouped with type strain. Strain OS14 showed the highest fiber digestion alone or combination with non-fibrolytic bacteria and higher than type strain. DM digestibility of cellulose powder in co-culture of OS14 with all strains non-fibrolytic was increased. Moreover, co-culture of OS14 with S137 showed the highest fiber digestion and notably increased of acetate and propionate. These results indicated that possible cross-feeding between R. flavefaciens with non-fibrolytic bacteria could improve fiber digestion but the amount of improvement might be based on the combination of bacteria strains. The in vitro fermentation products and abundance in co-culture need to be quantified in further study.

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Author Biography

Suriya Sawanon, Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand

Department of Animal Science, Faculty of Agriculture at Kamphaeng Saen

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Published

2019-06-29

How to Cite

Boonsaen, P., Poonko, S., Kanjanapruetipong, J., Phiriyangkul, P., & Sawanon, S. (2019). Isolation and partial characterization of Ruminococcus flavefaciens from the rumen of swamp buffalo. Buffalo Bulletin, 38(2), 311–325. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/667

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Original Article