Identification of IL-1α gene from bovine peripheral blood mononuclear cells by polymerase chain reaction

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S.D. Audarya A. Sanyal J.K. Mohapatra B. Pattnaik

Abstract

Interleukin-1 (IL-1) play its role in inflammation, fever and release of acute phase proteins by acting on major organs of the body. IL- 1α gene along with other cytokines compels Th1 and Th17 inflammatory responses. The present study is aimed at standardization of polymerase chain reaction for amplification of IL-1α gene from bovine peripheral blood mononuclear cells. Blood samples were collected in containers with 0.1% Ethylenediaminetetraacetic acid (EDTA) anticoagulant (Sigma, USA). Peripheral blood mononuclear cells (PBMCs) were isolated using commercially available Histopaque®-1077. Extracted total ribose nucleic acid (RNA) was utilized in reverse transcription to prepare complementary deoxyribose nucleic acid (cDNA). IL-1α gene specific primers were used to amlify partial nucleotide sequence of IL-1α. The cDNA was employed in polymerase chain reaction (PCR). The resulting IL-1α DNA product (124 bp) was identified on agar gel electrophoresis and documented.

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How to Cite
AUDARYA, S.D. et al. Identification of IL-1α gene from bovine peripheral blood mononuclear cells by polymerase chain reaction. Buffalo Bulletin, [S.l.], v. 36, n. 2, p. 323-326, june 2017. ISSN 2539-5696. Available at: <https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/696>. Date accessed: 19 may 2021.
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Original Article