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The aim of the present study was to evaluate the response of progesterone administration on resumption of cyclicity on post-partum anestrus buffaloes located in and around Danapur, Patna, Bihar area. Only those buffaloes were selected which did not show sign of estrus for one year after parturition and were maintained in small unorganized herd. The investigation was conducted in hot summer months (viz. March to June) season. The enumerated buffaloes were examined per rectally and a total of 38 apparently healthy buffaloes were selected with normal genitalia without having palpable corpus luteum on ovaries and pathological lesion. Selected animals were subjected to deworming prior to this study with broad-spectrum anthelmintic Fenbendazole (3 grams) once. The animals were first treated with PGF2α at the dose rate of (25 mg) intramuscularly on day ‘0’. Out of 38 animals 2 buffaloes that had shown signs of estrus have been dropped and they were discarded from further investigation. Finally total of 24 buffaloes were selected randomly and divided into 4 groups (6 animals in each group) to observe the effect of progesterone (Duraprogen, 250 mg) at different dosages on resumption of cyclicity. Animals of Group control were treated with normal saline (2 ml) on day 39, 43, 47 and 51 while those under Group T1,, T2 and T3 were treated with Duraprogen intramuscular on day 39. The animals of Group T1 were further treated with Duraprogen on day 41, 43, 45, 47 and 49 (i.e. on alternate day). The animals of Group T2 were further treated with Duraprogen on day 43, 47 and 51 (on three day interval) while the animals of group T3 were further treated on day 46 (on six day interval). Results showed that in group T1 , animals treated with progesterone (Duraprogen TM250 mg,1ml) I/M on alternate day (i.e. day 39, 41, 43, 45, 47 and 49), five out of 6 animals showed signs of estrus. Two animals showed signs of estrus on day 44 while other three showed the estrus sign on day 45, 46 and 49. The intensity of estrus was moderate in 2 animals and strong in 3 animals. In group T2 , where animals were treated with progesterone (Duraprogen TM250 mg, 1 ml) I/M on every 4th day (on day 39, 43, 47, 51), three out of 6 animals showed signs of estrus. Two animals showed sign of estrus on day 46 and one on day 50. The intensity of estrus was moderate in 2 animals and strong in 1 animal. In group T3 , where animals were treated with progesterone (Duraprogen TM250 mg) I/M on every 7th day after administration of 1st injection (on day 39 and 46), one out of 6 animals showed sign of estrus on day 43 and the intensity was moderate. Moreover, resumption of cyclicity was highly significant in group T1 in which repeated dosages of progesterone (Duraprogen TM250 mg, 1 ml) i.e. on alternate days, were administered. The effect of progesterone on cyclicity decreases with decrease in frequency of dose i.e. on three day interval and on six day interval.