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The present study was undertaken to know the sero prevalence of brucellosis in buffaloes in Telangana State by employing Rose Bengal plate test (RBPT), Lateral flow assay (LFA), Standard Tube agglutination test (STAT) and Enzyme linked immunosorbent assay (ELISA). Out of 920 buffaloes screened, 36 males and 884 females, 32 from Government farms and 888 from private farms and 32 from 1-3 years old, 844 from 3-6 years old and 44 from above 6 years old. The sero prevalence was 5.65%, 5.76%, 5.43% and 5.98% by RBPT, LFA, STAT and ELISA respectively. The prevalence in males was 5.56% by RBPT, LFA and ELISA, whereas 2.78% by STAT and in females, 5.66%, 5.77%, 5.54% and 6.00% by RBPT, LFA, STAT and ELISA respectively. The prevalence in Government farms was 3.13% by RBPT, LFA and ELISA whereas zero by STAT and from private farms 5.74%, 5.86%, 5.63 % and 6.08% were positive by RBPT, LFA, STAT and ELISA respectively. The prevalence in 1-3 years age was, 3.13% by RBPT, LFA and ELISA, zero by STAT, from 3-6 years age, 5.92%,6.04%, 5.81% and 6.16% by RBPT, LFA STAT and ELISA respectively and from above 6 years age 2.27% by RBPT, LFA and STAT, whereas 4.55%by ELISA. Higher efficacy observed by ELISA followed by LFA, RBPT and STAT. The higher prevalence was observed in females than males, in private than Government farms and in 3-6 years age group than other age groups.