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The aim of study is to diagnose the MCF and to study the clinical signs, haematobiochemical changes MCF in buflloes. The study revealed that occurrence of respiratory In the present study, buffaloes were more affected than cattle. Cultural isolation and identification of nasal discharges from the clinical cases revealed P. multocida, Pseudomonas spp., Klebsiella spp. and E. Coli. xviii. The P. multocida was also identified from the blood samples of the clinical cases by PCR. Twenty five buffaloes were found positive for malignant catarrhal fever with PCR. The most prominent clinical signs observed in bovine respiratory disease with bacterial involvement were respiratory distress, anorexia, nasal discharges, congested conjunctival mucous membrane, muzzle dryness, cough, oral breathing and sneezing. The prominent physical findings were dyspnoea, tachycardia, tachypnoea, pyrexia, crackles and wheezes. Haematological study showed normal haemogram and leukocytosis with neutrophilia. Serum biochemical profile showed hike in total protein and AST levels and fall in albumin levels. However eosinophils, lymphocytes and monocytes values were with in normal range. Thoracic radiography revealed consolidation of lung.