SEMINAL CHARACTERISTICS IN MURRAH BULLS RECOVERING FROM GOSSYPOL POISONING

Manisha Verma; Rajendra Prasad Tiwari; Girish Kumar Mishra; Mithilesh Uppal; Sanjeev Anand Sahasrabudhe; Shashi Bhushan Sahu

doi:10.56825/bufbu.2025.4444545

Authors

Manisha Verma Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Durg, India
Rajendra Prasad Tiwari Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Durg, India
Girish Kumar Mishra Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, India
Mithilesh Uppal Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Durg, India
Sanjeev Anand Sahasrabudhe Central Semen Station, Government of Chhattisgarh,Anjora, India
Shashi Bhushan Sahu Central Semen Station, Government of Chhattisgarh,Anjora, India

DOI:

https://doi.org/10.56825/bufbu.2025.4444545

Keywords:

Bubalus bubalis, buffaloes, cryopreservation, gossypol poisoning, seminal characteristics

Abstract

The present study was conducted on 5 Murrah buffalo bulls of Central Semen Station, Anjora, Durg. The objective was to analyze the semen properties, semen evaluation after thawing and effect of incubation in frozen thawed semen in Murrah bulls that are in recovery phase of gossypol poisoning. 20 semen ejaculates in total were taken (4 ejaculate from each bull) for the study. Seminal attributes recorded during the recovery period were compared with the data available in Central Semen Station (CSS), Anjora, Durg, Chhattisgarh for the period of poisoning and before poisoning. The average values of fresh semen trait in fresh semen during recovery period viz. semen volume (mL), sperm concentration (millions/mL), progressive initial sperm motility (%), live sperm (%), intact acrosome (%), total sperm abnormalities (%), hypo osmotic swelling (HOS %) and sperm penetration distance (SPD-mm) were 2.94±0.70, 1455.03±109.69, 72.50±1.38, 79.60±1.13, 77.93±0.95, 16.45±0.44, 59.00±0.89 and 27.35±0.62, respectively. There was no significant difference between the ejaculate volumes and sperm concentrations of Murrah bulls before the period of gossypol poisoning, during poisoning and during the recovery period. Progressive motility significantly declined (P<0.01) during poisoning period. There was no significant difference between the live sperm percentage and per cent normal acrosome before-poisoning and during the recovery phase while the per cent total abnormalities were significantly higher (P<0.05) during recovery period as compared to the before toxixity period. After freezing, the mean values of progressive sperm motility (%), total morphological sperm abnormalities (%), intact acrosome (%), HOS reactive (%) and SPD (mm) were 47.00±1.05, 18.70±0.39, 61.35±1.05, 51.55±0.66 and 21.60±0.43, respectively.

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Author Biographies

Manisha Verma, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Durg, India

Manisha Verma

Rajendra Prasad Tiwari, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Durg, India

Rajendra Prasad Tiwari

Girish Kumar Mishra, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, India

Girish Kumar Mishra*

drkodu@gmail.com

Mithilesh Uppal, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Durg, India

Mithilesh Uppa

Sanjeev Anand Sahasrabudhe, Central Semen Station, Government of Chhattisgarh,Anjora, India

Sanjeev Anand Sahasrabudhe
Central Semen Station, Government of Chhattisgarh,Anjora, India

Shashi Bhushan Sahu, Central Semen Station, Government of Chhattisgarh,Anjora, India

Shashi Bhushan Sahu

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