Evaluation of milk fat as an alternative source of RNA from buffalo milk

Main Article Content

Anju Sharma Yashila Girdhar Mandeep Kaur Sonika Ahlawat Ramesh Kumar Vijh Reena Arora

Abstract

The study reports optimization of RNA extraction method from buffalo milk fat, to be used for gene expression analysis. Higher yields were obtained from buffalo milk fat as compared to skim milk, with manual method as well as commercial kits. Quality and integrity of the samples from both methods was comparable. Quantitative profiling of mammary gland specific genes from skim milk and fat derived RNA validated the efficiency of MFG as an alternate source of total RNA. This study confirmed that the RNA obtained from buffalo MFG was suitable for studies of mammary genes expression.  

Downloads

Download data is not yet available.

Article Details

How to Cite
SHARMA, Anju et al. Evaluation of milk fat as an alternative source of RNA from buffalo milk. Buffalo Bulletin, [S.l.], v. 37, n. 2, p. 151-156, june 2018. ISSN 2539-5696. Available at: <http://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/553>. Date accessed: 22 sep. 2018.
Keywords
buffalo; milk; fat; qPCR; RNA
Section
Original Article