BIOMIMETIC DERMOID GRAFTS WITH ALLOGENIC BIOACTIVITY: AN INNOVATIVE APPROACH FOR HERNIA REPAIR IN BUFFALOES

Abstract

Hernia development is a common abdominal wall condition observed in calves, frequently manifesting in the umbilical region. This innovative biological dermoid scaffold is considered superior because of their inherent ability to combat infections through the release of antimicrobial peptides and non-complement fixing antibodies.

The Division of Surgery at ICAR-IVRI, Izatnagar, has pioneered the development of bioinspired-bioactive dermoid scaffolds, as an initiative aimed at addressing hernias in large animals. The in vitro evaluation of the scaffold included crucial parameters such as H and E staining, SEM evaluation, and scaffold-cell interaction. Histocompatibility, cyto-toxicity, cell attachment, and proliferation of the seeded scaffolds were assessed using DAPI staining, along with an examination of porosity and density.

All cases share a common history of a large swelling in the abdominal region. Physical examination, palpable hernial ring was identified followed by USG examination leading to the diagnosis of a large ventral hernia. Hematobiochemical analysis indicated a slight metabolic alkalosis, along with mild anaemia and azotaemia. Subsequent exploratory laparotomy was performed, to address the large abdominal defect, we decided to repair it using an allogenic dermoid graft in an overlaid fashion. Postoperative care included a 7-day course of antibiotics, analgesics, and antihistamines. All buffaloes in the study exhibited uneventful recovery, with no recurrence observed during the 5-month follow-up period.

No reports on treatment of large abdominal wall defects with dermoid graft reported, which prevent reoccurrence of hernia. This study marks for the first time, where we outline the successful closure of a hernia in clinical cases.