KVK, GUNTUR IMPACTFUL INTERVENTION WITH SEX-SORTED SEMEN: A FIELD STUDY ON CONCEPTION PERFORMANCE AND FEMALE CALF YIELD IN BUFFALOES
DOI:
https://doi.org/10.56825/bufbu.2026.4516447Keywords:
Bubalus bubalis, buffaloes, sex sorted semen, graded Murrah buffalo, conception rate, female calf, Articial inseminationAbstract
A field study was conducted under the impactful interventions of Keishi Vigyan Kendra (KVK), to popularise and evaluate the performance of Sex Sorted Semen (SSS) in Graded Murrah (GM) buffaloes under farmer managed field conditions of Guntur District, Andhra Pradesh. A total 600 doses of SSS straws derived from three breeding bulls (Mahabali, GM401, GM404) were used between November 2023 to June 2024 across two adopted villages of KVK. The inseminations were carried out as per the guidelines given by Andhra Pradesh Livestock Development Agency (APLDA) in enrolled GM buffaloes receiving either single or double dose of SSS straws. Pregnancy diagnosis was done between 80 to 100 days post insemination and animals were monitored for possibility of dystocia, still births and calf sex. Out of 393 inseminated buffaloes, 139 animals were found pregnancy positive (PD +ve) resulting in birth of 120 female and 13 male calves while six pregnant animals were sold before calving. Conception rates ranged from 25 to 38.71% across months, with a tendency of higher success (PD +ve) in double insemination as compared to single inseminations. Bull wise conception rate varied from 25 to 38.9% reflecting interactive effect of bull efficiency, seasonal variations, time of inseminations and insemination protocol. Overall, this trial achieved a conception rate of 35.4% with 90.2% female and 9.77% male calf births. Minimum reproductive complications were recorded (1.44% dystocia, 2.16% abortion cases and no still births or premature calves). These findings confirm the practical utility of SSS in reliably skewing the sex rate towards female calves, there by supporting herd replacement and sustainable dairy productivity under field conditions.
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References
Amann, RP. 1999. Issues affecting commercialization of sexed sperm. Theriogenology, 52(8): 1441-1457. DOI: 10.1016/s0093-691x(99)00229-0
Biswas, J., A. Chakrabarti, A. De, M. Pal, U.S. Das, K. Saha, A. Mondal and S. Pan. 2016. Minimum number of sex-sorted frozen sperm per dose in Sahiwal (Bos indicus) cattle. Adv. Anim. Vet. Sci., 4(12): 613-618. Available on: https://researcherslinks.com/nexus_uploads/files/AAVS_4_12_613-618.pdf
Campanile, G., B. Gasparrini, D. Vecchio, G. Neglia, E.M. Senatore, A. Bella, G.A. Presicce and L. Zicarelli. 2011. Pregnancy rates following AI with sexed semen in Mediterranean Italian buffalo heifers (Bubalus bubalis). Theriogenology, 76(3): 500-506. DOI: 10.1016/j.theriogenology.2011.02.029
Cerchiaro, I., M. Cassandro, R. Dal Zotto, P. Carnier and L. Gallo. 2007. A field study on fertility and purity of sex-sorted cattle sperm. J. Dairy Sci., 90(5): 2538-2542. DOI: 10.3168/jds.2006-694
Chebel, R.C., F.S. Guagnini, J.E.P. Santos, J.P. Fetrow and J.R. Lima. 2010. Sex-sorted semen for dairy heifers: Effects on reproductive and lactational performances. J. Dairy Sci., 93(6): 2496-2507. DOI: 10.3168/jds.2009-2858
DeJarnette, J.M., R.L. Nebel, C.E. Marshall, J.F. Moreno, C.R. McCleary and R.W. Lenz. 2008. Effect of sex-sorted sperm dosage on conception rates in Holstein heifers and lactating cows. J. Dairy Sci., 91(5): 1778-1785. DOI: 10.3168/jds.2007-0964
De Vries, A., M. Overton, J. Fetrow, K. Leslie, S. Eicker and G. Rogers. 2008. Exploring the impact of sexed semen on the structure of the dairy industry. J. Dairy Sci., 91(2): 847-856. DOI: 10.3168/jds.2007-0536
Gaviraghi, A., R. Puglisi, D. Balduzzi, A. Severgnini, V. Bornaghi, G. Bongioni, A. Frana, L.M. Gandini, A. Lukaj, C. Bonacina and A. Galli. 2013. Minimum number of spermatozoa per dose in Mediterranean Italian buffalo (Bubalus bubalis) using sexed frozen semen and conventional artificial insemination. Theriogenology, 79(8): 1171-1176. DOI: 10.1016/j.theriogenology.2013.02.014
Hirabhai, P.K., T.P. Hirjibhai, S.H. Hirjibhai and V.K. Bhagvanbhai. 2022. Seasonal variation in semen quality and conception rate of Jaffarabadi buffalo bulls (Bubalus bubalis) in India. Buffalo Bull., 41(3): 431-439. DOI: 10.56825/bufbu.2022.4133642
Johnson, L.A. and G.R. Welch. 1999. Sex preselection: high-speed flow cytometric sorting of X and Y sperm for maximum efficiency. Theriogenology, 52(8): 1323-1341. DOI: 10.1016/s0093-691x(99)00220-4
Kunavongkrit, A., A. Suriyasomboon, N. Lundeheim, T.W. Heard and S. Einarsson. 2005. Management and sperm production of boars under differing environmental conditions. Theriogenology, 63(2): 657-667. DOI: 10.1016/j.theriogenology.2004.09.039
Lakshmi, R.K.S., M.Y. Kumar, A. Anitha and S.N. Meera. 2025. Assessment of paddy straw availability as a feed source for sustainability of livestock farming in Guntur, Bapatla and Palnadu districts of Andhra Pradesh. Indian J. Anim. Nutr., 42(1). DOI: 10.56093/ijan.v42i1.3
Lu, Y., Y. Liao, M. Zhang, B. Yang, X. Liang, X. Yang, S. Lu, Z. Wu, H. Xu, Y. Liang and K. Lu. 2015. A field study on artificial insemination of swamp and crossbred buffaloes with sexed semen from river buffaloes. Theriogenology, 84(6): 862-867. DOI: 10.1016/j.theriogenology.2015.05.022
Seidel, Jr, G.E. and J.L. Schenk. 2008. Pregnancy rates in cattle with cryopreserved sexed sperm: Effects of sperm numbers per inseminate and site of sperm deposition. Anim. Reprod. Sci., 105(1-2): 129-138. DOI: 10.1016/j.anireprosci.2007.11.015
Sharma, N., D.K. Chand, S. Rawat, M. Sharma and H. Verma. 2018. Effect of sexed semen on conception rate and sex ratio under field conditions. J. Entomol. Zool. Stud., 6(1): 702-705. https://www.entomoljournal.com/archives/2018/vol6issue1/PartJ/6-1-26-860.pdf
Sharma, R., M.H. Khan, M.K. Patra, B. Kumar, N.P. Bajia, A.L. Reddy, N. Biswas and S. Kumar. 2024. Status and prospects of sex sorted semen in India. Int. J. Vet. Sci. Anim. Husb., 9(5): 39-47. DOI: 10.22271/veterinary.2024.v9.i5a.1645
Tubman, L.M., Z. Brink, T.K. Suh and G.E. Seidel Jr. 2004. Characteristics of calves produced with sperm sexed by flow cytometry/cell sorting. J. Anim. Sci., 82(4): 1029-1036. DOI: 10.2527/2004.8241029x
Weigel, K.A. 2004. Exploring the role of sexed semen in dairy production systems. J. Dairy Sci., 87(Suppl.): 120-130. DOI: 10.3168/jds.S0022-0302(04)70067-3
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