Molecular detection of Brucella abortus using BSCP31 and IS711 gene based PCR assay in cattle and buffalo

Authors

  • Kirit B. Patel Department of Animal Biotechnology and Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India
  • H.C. Chauhan Department of Animal Biotechnology and Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India
  • S.S. Patel Department of Animal Biotechnology and Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India
  • B.K. Patel Department of Animal Biotechnology and Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India
  • M.D. Shrimali Department of Animal Biotechnology and Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India
  • B.S. Chandel Department of Animal Biotechnology and Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India

Keywords:

Bubalus bubalis, buffaloes, Brucellosis, molecular detection, PCR, bscp31, B4/B5, IS711 primer

Abstract

Brucellosis has been one of the most important reproductive diseases among different livestock species as well as animal handlers and is considered a reemerging infectious disease in many areas of the world. A total of 353 clinical samples of cattle (207) and buffalo (146) were collected for molecular detection of Brucella. Out of 353 clinical samples, 6 samples (vaginal discharge-2, placenta-2, foetal stomach content-1, fetal lung-1) positive for Brucella organism by genus specific PCR based on BSCP31 (B4/B5 primer, 223bp). All six genus specific positive sample also positive for B. abortus +IS711 (498bp) species specific PCR. All the samples which were detected positive in conventional PCR also detected positive for Brucella in SYBR green and TaqMan probe based real time PCR.

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Published

2018-03-29

How to Cite

Patel, K. B., Chauhan, H., Patel, S., Patel, B., Shrimali, M., & Chandel, B. (2018). Molecular detection of Brucella abortus using BSCP31 and IS711 gene based PCR assay in cattle and buffalo. Buffalo Bulletin, 37(1), 71–80. Retrieved from http://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/907

Issue

Vol. 37 No. 1 (2018): January-March

Section

Original Article

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