Molecular detection of Brucella abortus using bscp31 and IS711 gene based pcr assay in cattle and buffalo

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Kirit B. Patel H.C. Chauhan S.S. Patel B.K. Patel M.D. Shrimali B.S. Chandel

Abstract

Brucellosis has been one of the most important reproductive diseases among different livestock species as well as animal handlers and is considered a reemerging infectious disease in many areas of the world. A total of 353 clinical samples of cattle (207) and buffalo (146) were collected for molecular detection of Brucella. Out of 353 clinical samples, 6 samples (vaginal discharge-2, placenta-2, foetal stomach content-1, fetal lung-1) positive for Brucella organism by genus specific PCR based on bscp31 (B4/B5 primer, 223bp). All six genus specific positive sample also positive for B. abortus +IS711 (498bp) species specific PCR. All the samples which were detected positive in conventional PCR also detected positive for Brucella in SYBR green and TaqMan probe based real time PCR.

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How to Cite
PATEL, Kirit B. et al. Molecular detection of Brucella abortus using bscp31 and IS711 gene based pcr assay in cattle and buffalo. Buffalo Bulletin, [S.l.], v. 37, n. 1, p. 71-80, mar. 2018. ISSN 2539-5696. Available at: <http://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/907>. Date accessed: 22 sep. 2018.
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Original Article