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In the present study 3779 feacal samples of buffaloes were collected from the seven agroclimatic zones of Madhya Pradesh state, India. The study was conducted for a period of one year from April 2011 to March 2012. The prevalence of gastrointestinal (GI) parasitic infection was 55.65% (2143). Amphistomes (28.10%) being the most prevalent GI parasite followed by Strongyle (25.59%), Schistosoma sp. (5.19%), Strongyloides sp. (3.15%), Trichuris sp. (2.59%), Fasciola sp. (2.30%), Toxocara (0.66%) and Monezia sp. (0.42%). Among non helmithic infection coccidian showed prevalence of 19.00%. Out of the seven zones, zone V (Central Narmada valley) had the highest prevalence (61.46%) and the Hills of Jhabua zone XII had the lowest prevalence (50.42%). Prevalence in calves was more (59.78%) as compared to adult (54.36%). Season wise highest prevalence was observed in monsoon (73.41%) followed by winter (60.47%) and then summer (36.22%). Prevalence of coccidiosis (25.00%) was highest in winter. Monthly prevalence data showed highest prevalence in the month of August (79.68%) and lowest in the month of April (28.25%). Mean EPG of strongylewas 321.8 and highest intensity of strongyle infection was recorded in the month of July (513.1). Coproculture examination revealed that Haemonchus being the predominant (72.08%) nematode genus, followed by Trichostrongylus (11.42%), Oesophagostomum (10.08%), Bunostomum (3.75%) and Strongyloides (2.67%). The current investigation provide basis to formulate strategic control measures against GI parasitism.