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A comparative studies on certain stress markers viz. SOD (Superoxide Dismutase), GSH (Total Glutathione) and GPx (Glutathione Peroxidase) as well as trace minerals viz. Cu (Copper), Co (Cobalt), Zn (Zinc) and Fe (Iron) were carried out on twenty four (24) lactating Surti buffaloes, divided into two groups viz. normal cyclic (n=12) and anoestrus (n=12) groups, maintained at Livestock Research Station, Navsari Agricultural University, Navsari, Gujarat. The mean SOD and GPx levels were found significantly (P<0.05) lower as 2.21±0.31 vs. 3.37±0.39 U/ml and 1.94±0.20 vs. 2.81±0.27 mU/ml, respectively whereas, the mean plasma GSH level was observed significantly (P<0.05) higher 56.32±1.21 vs. 51.49±1.38 ng/μl in normal cyclic as compared to anoestrus Surti buffaloes. The mean serum Cu and Zn concentration were observed significantly (P<0.01) higher 0.58±0.03 vs. 0.42±0.03 and 1.48±0.12 vs. 0.94±0.07 μg/ml, respectively and mean serum Co and Fe concentration were found non-significantly (P>0.05) higher 0.65±0.06 vs. 0.58±0.04 and 3.13±0.18 vs. 2.88±0.13 μg/ ml, respectively in normal cyclic as compared to anoestrus Surti buffaloes.