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Rabies is an infectious, fatal viral disease caused by a neurotropic virus and transmissible to all mammals through bite by rabid animals viz. dog, mongoose etc. In the present study rabies was tentatively diagnoses on the basis of history and clinical manifestations from buffalo of an organized farm of north Gujarat. This article summarizes the typical characteristics of rabies in a buffalo including its confirmatory diagnosis using florescent antibody test (FAT) and histopathology, which were consider as gold standard test for rabies diagnosis. Finding was also confirmed using polymerase chain reaction. Based on the study it was hypothesized that it might have been transmitted from rabid dog or mongoose.