Effect of supplementation of probiotics and rumen buffer on performance of lactating buffaloes

Main Article Content

Dharmendr Kumar Verma Ganesh Mahadeo Gadegaonkar Bhooshan Namdeorao Ramteke Swati Jaiswal Suresh Dattatray Jagadale Ganesh Darban


The study involved 12 Murrah buffaloes which were divided into two groups of six each viz. Control (T0) and Treatment group (T1). The buffaloes from Group T0 received standard concentrate mixture and roughages as per the routine practice of the farm. Whereas Group T1 received same ration as that of Group T0 along with supplement containing probiotics like yeast (Saccharomyces cerevisiae), bacteria (Bacillus subtilis, Bacillus pumilus and Bacillus amyloliquifaciens) and rumen buffer 10 g/day/animal. The experiment lasted for 91 days. The average daily milk yield and FCM yield, DMI, TDN and DCP intake of buffaloes from Treatment group (T1) was significantly (P˂0.01) higher than Control group. The efficiency of feed utilization in terms of dry matter required per kg of FCM yield produced was significantly (P˂0.01) better in Group T1 than Control group. The average milk fat, protein, total solids, SNF and lactose percentage of Group T1 was significantly (P˂0.01) higher than Control group. The average somatic cell count of milk of buffaloes form Group T1 was significantly (P˂0.05) lower than Control group. The economics of the study showed an extra profit of ₹ 25.20 in Supplemented group over Control group.


Download data is not yet available.

Article Details

How to Cite
VERMA, Dharmendr Kumar et al. Effect of supplementation of probiotics and rumen buffer on performance of lactating buffaloes. Buffalo Bulletin, [S.l.], v. 41, n. 2, p. 293-298, june 2022. ISSN 2539-5696. Available at: <https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/3080>. Date accessed: 16 aug. 2022.
Original Article


Alzahal, O., H. McGill, A. Kleinberg, J.I. Holliday, I.K. Hindrichsen, T.F. Duffield and B.W. McBride. 2014. Use of a direct-fed microbial product as a supplement during the transition period in dairy cattle. J. Dairy Sci., 97(11): 7102-7114. DOI: 10.3168/jds.2014-8248

A.O.A.C. 2005. Officially Analytical Chemists, 18th ed. Association of Analytical Chemistry, Washington D.C., USA.
Ayad, M.A., B. Benallou, M.S. Saim, M.A. Smadi and T. Meziane. 2013. Impact of feeding yeast culture on milk yield, milk components, and blood components in algerian dairy herds. Journal of Veterinary Science and Technology, 4(2): 1-5. DOI: 10.4172/2157-7579.1000135

Azzaz, H.H., H.M. Ebeid, T.A. Morsy and S.M. Kholif. 2015. Impact of feeding yeast culture or yeast culture and Propionibacteria 169 on the productive performance of lactating buffaloes. International Journal of Dairy Science, 10(3): 107-116. DOI: 10.3923/ijds.2015.107.116

BIS. 1990. Compounded Feeds for Cattle. Bureau of Indian Standards, New Delhi, India. Available on: https://www.bis.gov.in/index.php/old_product_manuals/compounded-feeds-for-cattle

Clark, J. H., R.A. Christensen, H.G. Bateman II and K.R. Cummings. 2009. Effects of sodium sesquicarbonate on dry matter intake and production of milk and milk components by Holstein cows. J. Dairy Sci., 92(7): 3354-3363. DOI: 10.3168/jds.2008-1995

Degirmencioglu, T., T. Ozcan, S. Ozbilgin and S. Senturklu. 2013. Effects of yeast culture addition (Saccharomyces cerevisiae) to Anatolian water buffalo diets on milk composition and somatic cell count. Mljekarstvo, 63(1): 42-48. Available on: https://hrcak.srce.hr/file/144672

Hossain, F.M.A., M.M. Islam, A. Ara and N. Iliyas. 2014. Supplementing probiotics (Saccharomyces cerevisiae) in multiparous crossbred cows ration provoke milk yield and composition. Online Journal of Animal and Feed Research, 4(2): 18-24. Available on:

Luan, S., M. Duersteler, E.A. Galbraith and F.C. Cardoso. 2015. Effects of direct-fed Bacillus pumilus 8G-134 on feed intake, milk yield, milk composition, feed conversion, and health condition of pre- and postpartum Holstein cows. J. Dairy Sci., 98(9): 6423-6432. DOI: 10.3168/jds.2015-9512

Maamouri, O., H. Selmi and N. M’hamdi. 2014. Effects of yeast (Saccharomyces cerevisiae) feed supplement on milk production and its composition in Tunisian Holstein Friesian cows. Scientia agriculturae bohemica, 45(3): 170-174. Available on: https://sciendo.com/pdf/10.2478/sab-2014-0104

Musa, A.A. 2017. Effects of feeding sodium bicarbonate and multi-strain probiotics on milk yield and milk composition of lactating Holstein Frisian crossbred cows. Journal of Pharmacognosy and Phytochemistry, 6(6): 1912-1916. Available on: https://www.phytojournal.com/archives/2017/vol6issue6/PartAA/6-6-187-115.pdf

Raafat, M.S. and M.E. Saleh. 1962. Efficiency of feed utilization with buffaloes and dairy cattle. In Proceeding of 2nd Animal Production Conference, Cairo, Egypt.

Snedecor, G.W. and W.G. Cochran. 1994. Statistical Methods, 8th ed. Iowa State University Press, Ames, Iowa, USA.

Talapatra, S.K., S.C. Ray and K.C. Sen. 1940. The analysis of mineral constituents in biological materials. 1. Estimation of phosphorus, chlorine, calcium, magnesium, sodium and potassium in food-stuffs. Indian J. Vet. Sci., 10: 243-258.