Correlation of age with body weight and testicular biometry in developing buffalo males of Murrah breed


  • Lal Chand Ranga Haryana Livestock Development Board, Panchkula, India
  • Ramesh Kumar Chandolia Department of Veterinary Gynaecology and Obstetrics, Lala Lajpat Rai University of Veterinary and Animal Sciences, Haryana, India
  • Laxman Singh Dr. Bhimrao Ambedkar University, Bichpuri, Uttar Pradesh, India
  • Ravi Dutt Department of Veterinary Gynaecology and Obstetrics, Lala Lajpat Rai University of Veterinary and Animal Sciences, Haryana, India



Bubalus bubalis, buffaloes, body weight, biometry, scrotal circumference, testicular length, width


The current investigation was conducted on one hundred and eighty Murrah buffalo male calves from the age of one to thirty months, to investigate the correlation of age with body weight and testicular biometry. Six animals were included in each month. The body weight (b. wt) of the male calves was recorded on the weight bridge balance and the circumference of scrotum was measured by inch tape. The testicular dimensions were measured ultasonographically. Mean body weight from one to thirty month ranged between 57.0±1.99 and 433.0±9.81 kg. It increased 12.52 kg per month. The mean testicular circumference at 1, 12, 18 and 30 months was 9.00±00, 20.25±0.64, 21.42±0.64 and 26.67±0.42 cm, respectively. The overall increase in testicular circumference from one to thirty months was 0.59 cm per month. The mean length of right testes at one month was 2.89±0.15 cm and at eighteenth month was 6.87±0.27 cm. It increased in parallel pattern with b.wt and age. The width of right testes ranged between 1.01±0.05 to 4.53±0.211 cm. The length of left testes from one to eighteen month ranged between 2.73±0.19 to 6.49±0.10 cm with an increase of 0.21 cm per month. Similarly, the mean width of left testes ranged between 1.12±0.02 to 4.61±0.17 cm and significantly increased 0.12 cm/month. The data obtained in the current extensive study may serve as reference values for Murrah buffalo bulls.


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Abdel-Razek, A.K. and A. Ali. 2005. Developmental changes of bull (Bos taurus) genitalia as evaluated by caliper and ultrasonography. Reprod. Domest. Anim., 40(1): 23-27. DOI: 10.1111/j.1439-0531.2004.00549.x

Ahmad, E., N. Ahmad, Z. Naseer, M. Aleem, M.S. Khan, M. Ashiq and M. Younis. 2011. Relationship of age to body weight, scrotal circumference, testicular ultrasonograms, and semen quality in Sahiwal bulls. Trop. Anim. Health Pro., 43(1): 159-164. DOI: 10.1007/s11250-010-9668-1

Ahmad, M., M.T. Asmat and N.U. Rehman. 2005. Relationship of testicular size and libido to age and season in Sahiwal bulls. Pak. Vet. J., 25(2): 67-70. Available on:

Alexander, J.H. 2008. Bull breeding soundness evaluation: A practitioner’s perspective. Theriogenology, 70(3): 469-472. DOI: 10.1016/j.theriogenology.2008.05.030

Almquist, J.O., R.J. Branas and K.A. Barber. 1976. Postpuberal changes in semen production of Charolais bulls ejaculated at high frequency and the relation between testicular measurements and sperm output. J. Anim. Sci., 42(3): 670-676. DOI: 10.2527/jas1976.423670x

Arrighi, S., G. Bosi, D. Groppetti and F. Cremonesi. 2010. Morpho-and Histometric Evaluations on the testis and epididymis in buffalo bulls during the different reproductive seasons. The Open Anatomy Journal, 2(1): 29-33. DOI: 10.2174/1877609401002010029

Barth, A.D. 2007. Evaluation of potential breeding soundness of the bull. In Youngquist, R.S. and W.R. Thelwall (eds). Current Therapy in Large Animal Theriogenology, Saunders Elsevier, Philadelphia, USA. p. 228-240.

Basic Animal Husbandry Statistics. 2019. Ministry of Fisheries, Animal Husbandry and Dairying, Department of Animal Husbandry and Dairying, Krishi Bhawan, New Delhi, India. Available on:

Bedi, K.S. 1980. Studies on genital development and semen quality in buffalo bulls. M.VSc. Thesis, Punjab Agricultural University, Ludhiana, India.

Brito, L.F.C., A.E.D.F. Silva, L.H. Rodrigues, F.V. Vieira, L.A.G. Deragon and J.P. Kastelic. 2002. Effect of age and genetic group on characteristics of the scrotum, testes and testicular vascular cones, and on sperm production and semen quality in AI bulls in Brazil. Theriogenology, 58(6): 1175-1186. DOI: 10.1016/s0093-691x(02)00921-4

Cardoso da Luz, P.A., P.R. Santos, C. Andrighetto, A.M. Jorge and A.C. Assis Neto. 2013. The correlation between age, body weight and testicular parameters in Murrah buffalo bulls raised in Brazil. J. Reprod. Develop., 59(1): 14-17. DOI: 10.1262/jrd.2012-021

Chandolia, R.K., A. Honaramooz, B.C. Omeke, R. Pierson, A.P. Beard and N.C. Rawlings. 1997. Assessment of development of the testes and accessory glands by ultrasonography in bull calves and associated endocrine changes. Theriogenology, 48(1): 119-132. DOI: 10.1016/S0093-691X(97)00195-7

Chenoweth, P.J., F.M. Hopkins, J.C. Spitzer and R.E. Larsen. 2010. Guidelines for using the bull breeding soundness evaluation form. Clinical Theriogenology, 2(1): 43-50.

Crudeli, G., D. Pochon, M. Olazarri, N. Monzón, L. Chaparro, S. Flores, E. Patiño and J. Cedres. 2007. Morphometric evaluation of male Mediterranean buffaloes in Northern Corrientes, Argentina. Ital. J. Anim. Sci., 6: 1281-1283. DOI: 10.4081/ijas.2007.s2.1281

Devkota, B., T. Koseki, M. Matsui, M. Sasaki, E. Kaneko, A. Miyamoto, C.A. Montoya and Y.I. Miyake. 2008. Relationships among age, body weight, scrotal circumference, semen quality and peripheral testosterone and estradiol concentrations in pubertal and postpubertal Holstein bulls. J. Vet. Med. Sci., 70(1): 119-121. DOI: 10.1292/jvms.70.119

Galmessa, U. 2002. Semen quality and cryopreservability in relation to testicular biometry in cattle. M.Sc. Thesis, Indian Council of Agricultural Research- National Dairy Research Institute, Deemed University, Haryana, India.

Gipsona, T.A., D.W. Vogtl and J.W. Massey. 1985. Associations of scrotal circumference with semen traits in young beef bulls. Theriogenology, 24(2): 217-225. DOI: 10.1016/0093-691x(85)90186-4

Henry, M., F.M. Brito, A.M. Loaiza Echeverri, C.H.S. De Oliveira, A.F.B. Gibson, B.P. Neves, G.D.O. Andrade, O.I. Meloand and E. Bastianetto. 2013. Scrotal circumference growth curves of buffalo bulls of different breeds raised in Brazil. Buffalo Bull., 32(2): 439-442. Available on:

Jain, R. 2004. Relationship of age, body weight, testicular measurement and semen quality for selection of Karan Fries and Sahiwal bulls. M.Sc. Thesis, Indian Council of Agricultural Research, National Dairy Research Institute, Deemed University Karnal, Haryana, India.

Kumar, H. 2014. Effect of ascorbic acid on refrigerated storage capacity of epididymal spermatozoa of Murrah bull. M.V.Sc. Thesis, Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India.

Mahmood, S.A., A. Ijaz, N. Ahmed, H. Rehman, H. Zaneb and U. Farooq. 2014. A study on relationships among age, body weight, orchidometry and semen quality parameters in adult Cholistani breeding bulls. J. Anim. Plant Sci., 24(2): 380-384. Available on:

Mathur, A.K., D.K. Mandal, M. Kumar and S. Tyagi. 2006. Biometry of testes in Frieswal bulls. Indian J. Anim. Sci., 76(1): 38-40.

Montes, I., I. Pérez and J.G. Martinto. 2007. Desarrollo corporal y testicular del búfalo hasta los 11 meses de nacidosen Cuba. Ciencia y Tecnología Ganadera. 1: 35-40.

Pant, H.C., R.K. Sharma, S.H. Patel, S.H. Shukla, A.K. Mittal, R. Kasiraj, A.K. Mishra and J.H. Prabhakar. 2003. Testicular development and its relationship to semen production in Murrah buffalo bulls. Theriogenology, 60(1): 27-34. DOI: 10.1016/s0093-691x(02)01037-3

Perumal, P. 2014. Scrotal circumference and its relationship with testicular growth, age, and body weight in Tho Tho (Bos indicus) bulls. International Scholarly Research Notices, 2014(29). DOI: 10.1155/2014/249537

Raji, A.O., J.U. Igwebuike and J. Aliyu. 2008. Testicular biometry and its relationship with body weight of indigenous goats in semi arid region of Nigeria. American Journal of Agricultural and Biological Sciences, 3(4): 6-9.

Sharma, A.K. 1987. Studies on the process of reproduction, reproductive capacity and extra gonadal sperm transit time in buffalo bull. Ph.D. Thesis, Haryana Agricultural University Hisar, Haryana, India.

Shende, V.H., S.H. Sontakke, V.V. Potdar, T.V. Shirsath and J.R. Khadse. 2019. Scrotal circumference and its relationship with testicular growth, age, and body weight in Murrah buffalo bulls. International Journal of Current Microbiology and Applied Sciences, 8(11): 2270-2274. DOI: 10.20546/ijcmas.2019.811.264

Siddiqui, A.R., J. Bhattacharjee, Z.C. Das, M.M. Islam, M.A. Islam, M.A. Haque, J.J. Parrish and M. Shamsuddin. 2008: Crossbred bull selection for bigger scrotum and shorter age at puberty with potentials for better quality semen. Reprod. Domest. Anim., 43(1): 74-79. DOI: 10.1111/j.1439-0531.2007.00857.x

Siqueira, J.B., E. Oba, R.O. Pinho, S.E.F. Guimaraes, T.M. Neto and J.D. Guimaraes. 2012. Testicular shape and andrological aspects of young Nellore bulls under extensive farming. Rev. Bras. Zootecn., 41(3): 612-617. DOI: 10.1590/S1516-35982012000300020

Sudheer, S. 2000. Relationship of age and scrotal circumference with the seminal characteristics of crossbred bulls. Indian J. Anim. Sci., 70(8): 804-806.

Suri, A.K. 1993. Neonate and pubertal androgen-leutenizing hormone relationship in male calves. Ph.D. Thesis, Indian Council of Agricultural Research, National Dairy Research Institute, Deemed University, Karnal, Haryana, India.

Sylla, L., G. Stradaioli, S. Borgami and M. Monaci. 2007. Breeding soundness examination of Chianina, Marchigiana, and Romagnola yearling bulls in performance tests over a 10-year period. Theriogenology, 67(8): 1351-1358. DOI: 10.1016/j.theriogenology.2007.02.007

Togun, V.A. and G.N. Egbunike. 2006. Seasonal variations in the sperm production characteristics of Zebu (White Fulani) cattle genitalia in the humid tropical environment. Middle-East Journal of Science Research, 1: 87-95.

Torres-Júnior, J.R.S. and M. Henry. 2005. Sexual development of Guzerat (Bos taurus indicus) bulls raised in a tropical region. Anim. Reprod., 2(2): 114-121.

Varghese, M.R., M.A. Kataktalware, S. Jeyakumar, D.N. Das, K.P. Ramesha and P.R. Wankhade. 2019. Testicular biometry and its relationship with age and body weight in young Deoni males. Indian J. Anim. Res., 53(12): 1624-1628. DOI: 10.18805/ijar.B-3703

Viu, M.A.O., C.U. Magnabosco, B.D.O. Filho., D.T. Lopes, R.N.G. Porto, H.T. Ferraz and M.L. Gambarini. 2015. Testicular biometry, semen quality and sexual maturity of Nellore young bulls raised in the Brazilian tropical savannah. J. Anim. Vet. Adv., 14(6): 144-150. DOI: 10.3923/javaa.2015.144.150

Waldner, C.L., R.I. Kennedy and C.W. Palmer. 2010. A description of the findings from bull breeding soundness evaluations and their association with pregnancy outcomes in a study of western Canadian beef herds. Theriogenology, 74(5): 871-883. DOI: 10.1016/j.theriogenology.2010.04.012




How to Cite

Chand Ranga, L., Kumar Chandolia, R., Singh, L., & Dutt, R. (2023). Correlation of age with body weight and testicular biometry in developing buffalo males of Murrah breed. Buffalo Bulletin, 42(4), 455–466.



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