Fertility response in subclinical endometritic Murrah buffaloes treated with different therapeutic regimens
DOI:
https://doi.org/10.56825/bufbu.2024.4334509Keywords:
Bubalus bubalis, buffaloes, subclinical endometritis, endometrial cytology, fertility response, cytological endometritis, polymorphonuclear cellsAbstract
The present study was planned to study the therapeutic efficacy of different therapeutic regimes for enhancing uterine immunity and fertility response in Murrah buffaloes suffering from sub-clinical endometritis. Thirty postpartum buffaloes (28 to 45 days postpartum) found positive for sub-clinical endometritis by endometrial cytology in various dairy farms of Jabalpur (Madhya Pradesh) were included in the study. These animals were randomly divided into five treatment (n=06/group) and were subjected to different treatment regimen. Group 1 animals were intramuscularly injected with 500 mcg cloprostenol, Group 2 animals were given 500 mcg Cloprostenol intramuscularly + 500 mg single intrauterine infusion of cephapirin benzathin, Group 3 animals were given single intrauterine infusion of 100 mcg E. coli LPS in 30 ml sterile PBS solution, Group 3 animals were given single intrauterine infusion of 500 mg Oyster glycogen in 30 ml sterile PBS solution and Group IV animals were given single intrauterine infusion of 0.25% Lugol’s iodine (20 ml). All the 30 buffaloes were tracked for next successive oestrus and were again subjected to endometrial cytology and were bred by natural service and confirmed for pregnancy between 35 to 60 days post-breeding by trans-rectal ultrasonography.
The pre-treatment PMN percent varied significantly (P<0.05) between treatment groups. Significant decline (P<0.05) in post-treatment PMN percent was observed in all the treatment groups. The first service pregnancy rate was found to be highest (66.67%) in Group 3 and 4. Whereas overall pregnancy rate was higher (83.33%) in treatment Groups 2, 3 and 4.
Downloads
Metrics
References
Ahmed, F.O. and A.S. Elsheikh. 2014. Treatment of repeat breeding in dairy cattle with Lugol’s iodine. IOSR Journal of Agriculture and Veterinary Science, 7(4): 22-26. DOI: 10.9790/2380-07412226
Barlund, C.S., T.D. Carruthers, C.L. Waldner and C.W. Palmer. 2008. A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology, 69(6): 714-723. DOI: 10.1016/j.theriogenology.2007.12.005
Biswal, S.S., S. Das, D.N. Mohanty and G.R. Sahoo. 2014. Effect of immunomodulators on acute phase proteins and conception rate in endometritic cattle. In 29th Annual Convention and National Symposium on Frontier Reproductive Biotechnologies for Enhancing Animal Fertility and Fecundity: Global Prespective, Nagpur, Maharastra, India.
Boby, J., H. Kumar, H.P. Gupta, M.H. Jan, S.K. Singh, M.K. Patra, S. Nandi, A. Abraham and N. Krishnaswamy. 2017. Endometritis increases pro-inflammatory cytokines in follicular fluid and cervico-vaginal mucus in the buffalo cow. Anim. Biotechnol., 28(3): 163-167. DOI: 10.1080/10495398.2016.1244067
Brodzki, P., N. Krzysztof, K. Krzysztof, A. Brodzki, L. Kurek and J. Marczuk. 2014. Cytological evaluation of inflammation of the uterus and influence of endometritis on selected reproductive parameters in dairy cows. B. Vet. I. Pulawy, 58(2): 235-242. DOI: 10.2478/bvip-2014-0035
Dadarwal, D., J. Singh, M. Honparkhe, G.S. Dhaliwal and G.S. Cheede. 2007. Field efficacy of E. coli lipopolysaccharide intrauterine infusion in treating endometritis in crossbred cows. Indian J. Anim. Sci., 77(12): 1224-1237.
Drillich, M., D. Raab, W. Miriam and W. Heuwieser. 2005. Treatment of chronic endometritis in dairy cows with an intrauterine application of enzymes: A field trial. Theriogenology, 63(7): 1811-1823. DOI: 10.1016/j.theriogenology.2004.05.031
Dubuc, J., T.F. Duffield, K.E. Leslie, J.S. Walton and S.J. Leblanc. 2011. Randomized clinical trial of antibiotic and prostaglandin treatments for uterine health and reproductive performance in dairy cows. J. Dairy Sci., 94(3): 1325-1338. DOI: 10.3168/jds.2010-3757
Ghasemi, F., P. Gonzalez-Cano, P.J. Griebel and C. Palmer. 2012. Proinflammatory cytokine gene expression in endometrial cytobrush samples harvested from cows with and without subclinical endometritis. Theriogenology, 78(7): 1538-1547. DOI: 10.1016/j.theriogenology.2012.06.022
Kasimanickam, R., T.F. Duffield, R.A. Foster, C.J. Gartely, K.E. Leslie, J.S. Walton and W.H. Johnson. 2004. Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology, 62(1-2): 9-23. DOI: 10.1016/j.theriogenology.2003.03.001
Kasimanickam, R., T.F. Duffield, R.A. Foster, C.J. Gartley, K.E. Leslie, J.S. Walton and W.H. Johnson. 2005. A comparison of the cytobrush and uterine lavage techniques to evaluate endometrial cytology in clinically normal postpartum dairy cows. Canadian Vet. J., 46(3): 255-259.
Lee, S.C., J.K. Jeong, I.S. Choi, HG. Kang, Y.H. Jung, S.B. Park and H. Kim. 2018. Cytological endometritis in dairy cows: Diagnostic threshold, risk factors, and impact on reproductive performance. J. Vet. Sci., 19(2): 301-308. DOI: 10.4142/jvs.2018.19.2.301
Madoz, L.V., M.J. Giuliodori, A.L. Migliiorisi, M. Jaureguiberry and R.L. Sota. 2014. Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows. J. Dairy Sci., 97: 1-7. DOI: 10.3168/jds.2013-6836
Melcher, Y., I. Prunner and M. Drillich. 2014. Degree of variation and reproducibility of different methods for the diagnosis of subclinical endometritis. Theriogenology, 82(1): 57-63. DOI: 10.1016/j.theriogenology.2014.03.003
Prasad, J.K., M.S. Saxena, S. Prasad and G.K. Singh. 2009. Comparative efficacy of Escherichia coli lipopolysaccharide, oyster glycogen and enrofloxacin on uterine defense mechanism and fertility in crossbred cows with endometritis. Indian J. Anim. Sci., 79(11): 1111-1115.
Paisley, L.G., W.D. Mickelsen and P.B. Andersen. 1986. Mechanisms and therapy for retained fetal membranes and uterine infections of cows: A review. Theriogenology, 25: 353-381.
Rathod, S.B., A.P. Gawande, S.R. Chinckar, S.K. Sahatpure, M.S. Patil, R.P. Limsay, S.R. Warke, P.R. Sirsath and B.R. Ratnaparkhi. 2014. Comparative efficacy of levamisol and oyster glycogen on fertility of cross-bred cows with sub-clinical endometritis. In 29th Annual Convention and National Symposium on Frontier Reproductive Biotechnologies for Enhancing Animal Fertility and Fecundity: Global prespective, Nagpur, Maharastra, India.
Saini, P.S., A.S. Nanda, A.S. Grewal and A.K. Arora. 1995. Intrauterine immunotherapy with immunomodulator lipopolysaccharide (LPS) for bacterial endometritis associated clinical signs of repeat breeders in dairy cattle (Bos taurus x Bos indicus) and buffaloes (Bubalus bubalis bull). Jpn. J. Vet. Sci., 6(2): 128-134.
Sheldon, I.M. and H. Dobson. 2004. Postpartum uterine health in cattle. Anim. Reprod. Sci., 82-83: 295-306. DOI: 10.1016/j.anireprosci.2004.04.006
Singh, J. 2014. Immunomodulation therapy as an alternative approach to antibiotic therapy in endometritic dairy cattle. M.V.Sc. Thesis, Guru Angad Dev Veterinary Science University, Ludhiana, India.
Singh, H., P.S. Brar, M. Honparkhe, A.K. Arora and S.S. Dhindsa. 2019. Subclinical endometritis in estrual buffaloes: Diagnosis, prevalence and impact on reproductive performance. Trop. Anim. Health Pro., DOI: 10.1007/s11250-019-02022-w.
Singh, J., S.S. Sidhu, G.S. Dhaliwal, G.R. Pangaokar, A.S. Nanda and A.S. Grewal. 2000. Effectiveness of lipopolysaccharide as an intrauterine immunomodulator in curing bacterial endometritis in repeat breeding cross-bred cows. Anim. Reprod. Sci., 59(3-4): 159-166. DOI: 10.1016/s0378-4320(00)00144-5
Singla, P., J. Singh, G.S. Dhaliwal and A. Kumar. 2004. Effect of post AI intrauterine treatment with E. coli lipopolysachharide and autologous serum on characteristics of cervico-vaginal mucous in subclinical endometritic cows. Indian J. Anim. Sci., 25(1): 55-57.
Snedecor, G.W. and W.G. Cocharn. 1994. Statistical Methods, 8th ed. Iowa State University Press, Ames, USA.
Tizard, I.R. 1998. Veterinary Immunology an Introduction, 5th ed. Harcourt Brace and Co., Singapore. 531p.
Downloads
Published
How to Cite
Issue
Section
.png){kind=logo}
