Hematobiochemical alterations in clinical and subclinical hypophosphatemia in Egyptian buffaloes (Bubalus bubalis)
Keywords:
Bubalus bubalis, buffaloes, hematology, biochemistry, hypophosphatemiaAbstract
This study aimed to verify the hematological and biochemical alterations in buffaloes with clinical (CH) and subclinical hypophosphatemia (SCH). For this intention blood and serum samples from 40 adult female water buffaloes were collected. These animals were divided into 3 groups according to the clinical signs and serum phosphorous level. The first group was ten apparently clinically healthy buffaloes and was assigned as a normal control group. The second and the third groups were fifteen buffaloes for each CH and SCH groups. Anorexia, weakness, emaciation, and pale mucous membrane were the most clinical findings in all affected cattle. Red urine was an additional sign in buffaloes with CH. Mean erythrocytic count, hemoglobin concentration and hematocrit were significantly decreased in CH and SCH, while total leukocytic counts were significantly increased in both groups when compared with the control group. The main biochemical changes were significant decrease in serum levels of phosphorous, total proteins, albumin in diseased groups. Serum urea, creatinine, glucose, bilirubin were significantly elevated in both affected groups. Meanwhile, serum calcium level was significantly elevated only in the CH when compared with the control group. It could be concluded that SCH and CH are accompanied with anemia, changes in total and differential leukocytic counts, and alterations in the hepatic and kidney functions.
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References
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Al-Majalli. 2010. Clinical and biochemical. The 4th Congress of Egypt, Egypt.
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Benjamin, M.M. 1978. Outline of Veterinary Clinical Pathology, 3rd ed. Iowa State University Press, Ames, Iowa, USA.
Bhikane, A.U. and A.M. Syed. 2014. Recent trends in management of metabolic disorders of transition cows and buffaloes. Intas Polivet, 15(2): 485-496.
Bremmer, D.R., S.L. Trower and S.J. Bertics. 2000. Etiology of fatty liver in dairy cattle: Effects of nutritional and hormonal status on hepatic microsomal triglyceride transfer protein. J. Dairy Sci., 83(10): 2239-2251. DOI: 10.3168/jds.S0022-0302(00)75108-3
Digraskar, S., B. Singh and B.B. Deshpande. 1991. Epidemiology and clinico-pathology of hemoglobinuria in buffalo (Bubalus bubalis). Livestock Advisor, 16: 32-38.
Doumas, B.T., D.D. Bayse, R.J. Carter, T. Peters Jr and R. Schaffer. 1981. A candidate reference method for determination for total protein in serum. I. Development and validation. Clin. Chem., 27(10): 1642-1650.
Doumas, B.T., W.A. Watson and H.G. Biggs. 1971. Albumin standards and the measurement of serum albumin with bromocryzol green. Clin. Chem. Acta., 31(1): 87-96. DOI: 10.1016/0009-8981(71)90365-2
Durrani, A.Z., N. Kamal, A. Shakoori and R.M. Younus. 2010. Prevalence of post parturient haemoglobinuria in buffalo and therapeutic trials with toldimfos sodium and tea leaves in Pakistan. Turk. J. Vet. Anim. Sci., 34(1): 45-51.
Fayed, H., M. Ghanem, Y. Abdel-Raof and H.M. El-Attar. 2018. Hematobiochemical and urological alterations in buffaloes with post parturient haemoglobinuria. Benha Veterinary Medical Journal, 34(3): 287-294. DOI: 10.21608/BVMJ.2018.47846
Feldman, G.S., N.R. Perkins and N.C. Jain. 2000. Schalm’s Veterinary Heamatology, 4th ed. Lea and Fibiger, Philadelphia, USA.
Forrester, S.D. and K.J. Moreland. 1989. Hypophosphatemia. Causes and clinical consequences. J. Vet. Intern. Med., 3(3): 149-159. DOI: 10.1111/j.1939-1676.1989.tb03091.x
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Kurek, L., K. Lutnicki and A. Barch. 2010. Various types of hypophosphatemia in dairy cows and the clinical implications depending on the intensity of the deficiency. B. Vet. I. Pulawy, 54(1): 35-41.
Latimer, K.S., E.A. Mahaffey, K.W. Prasse and S. Ducan. 2003. Veterinary Laboratory Medicine: Clinical Pathology, 4th ed. Iowa State Press, Iowa, USA.
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Marik, P.E. and R. Bellomo. 2013. Stress hyperglycemia: An essential survival response. Crit. Care, 17(2): 305. DOI: 10.1186/cc12514
Muhammad, G., M. Saqib and M. Athar. 2001. A rational approach to diagnosis, treatment and control of parturient hemoglobinuria (red water) in buffaloes and cattle. Pak. Vet. J., 21(4): 214-219.
Navjot, S., K. Palneez, S. Harbir and Sh. Neelesh. 2017. Postpartum hemoglobinuria (PPH) in bovine. Theriogenology Insight, 7(1): 51-59. DOI: 10.5958/2277-3371.2017.00016.X
Purohit, G.N., G. Trilok, K. Amit, Sh. Atul, G. Mitesh and Sh. Chandra. 2018. Perspectives of parturient hemoglobinuria (pph) in buffaloes. International Journal of Development Research, 2: 23513-23520.
Rahmati, S., A. Aziz, M. Tawfeeq, J. Zabuli and S. Nazhat. 2021. Clinical features of post-parturient hemoglobinuria in dairy cattle and buffaloes. Open Journal of Veterinary Medicine, 11(4): 143-155. DOI: 10.4236/ojvm.2021.114010
Rana, J.P. and R.M. Bhardwaj. 1988. Relationship of intraerythrocytic ATP and membrane phospholipids to red cell shape in hemoglobinuric buffaloes. In The Proceeding 2nd World Buffalo Congress, New Delhi, India. p. 178-184.
Rock, R.C., W.G. Walker and C.D. Jennings. 1987. Nitrogen metabolites and renal function. In Fundamentals of Clinical Chemistry 3rd ed. WB Saunders, Philadelphia, USA.
Sarma, K., M. Saravanan, K. Pankaj, M. Kumar, R.K. Jadav and D.B. Monda. 2014. Influence on haemato-biochemical and oxidative indices of post parturient haemoglobinuric (PHU) buffalo. Buffalo Bull., 33(4): 343-348. Available on: https://ibic.lib.ku.ac.th/e-bulletin/IBBU201404013.pdf
Stockdale, C.R., T.E. Moyes and R. Dyson. 2005. Acute post-parturient haemoglobinuria in dairy cows and phosphorus status. Aust. Vet. J., 83(6): 362-266. DOI: 10.1111/j.1751-0813.2005.tb15635.x
Tamhane, A.C. and D.D. Dunlop. 2000. Statistics and Data Analysis from Elementary to Intermediate. Upper Saddle River, USA.
Tietz, N.W. 1990. Clinical Guide to Laboratory Tests, 2nd ed. WB Saunders Company, Philadelphia, USA. p. 444-447.
Tietz, N.W. 1995. Clinical Guide to laboratory Tests, 3rd ed. WB Saunders Company, Philadelphia, USA. p. 102.
Trinder, P. 1969. Enzymatic determination of glucose. Ann. Clin. Biochem., 6: 24-26.
Vassault, A., D. Grafmeyer, J. de Graeve, R. Cohen, A. Beaudonnet and J. Bienvenu. 1986. Protocol for the validation of methods. Ann. Biol. Clin.-Paris, 44: 686-745.
Wang, X.L., C.H. Gallager, T.J. Mcclure, V.E. Reeve and P.J. Canfield. 1985. Bovine post-parturient haemoglobinuria: Effect of inorganic phosphate on red cell metabolism. Res. Vet. Sci., 39(3): 333-339.
Zaghawa, A. Yousef and H, Nayel M. 2019. Clinical, epidemiological and clinic-pathological aspects of hypophosphatemia in buffaloes in Egypt. Bioscience Research, 16: 31-41.
Ziwei, Z., B.I. Mingyu and X.U. Shiwen. 2017. Effect of phosphorus deficiency on erythrocytic morphology and function in cows. Vet. Sci., 18(3): 333-340. DOI: 10.4142/jvs.2017.18.3.333
Al-Majalli. 2010. Clinical and biochemical. The 4th Congress of Egypt, Egypt.
Belfield, A. and D.M. Goldberg. 1971. Revised assay for serum phenyl phosphate activity using 4-amino-antipyrine. Enzyme, 12(15): 561-573. DOI: 10.1159/000459586
Benjamin, M.M. 1978. Outline of Veterinary Clinical Pathology, 3rd ed. Iowa State University Press, Ames, Iowa, USA.
Bhikane, A.U. and A.M. Syed. 2014. Recent trends in management of metabolic disorders of transition cows and buffaloes. Intas Polivet, 15(2): 485-496.
Bremmer, D.R., S.L. Trower and S.J. Bertics. 2000. Etiology of fatty liver in dairy cattle: Effects of nutritional and hormonal status on hepatic microsomal triglyceride transfer protein. J. Dairy Sci., 83(10): 2239-2251. DOI: 10.3168/jds.S0022-0302(00)75108-3
Digraskar, S., B. Singh and B.B. Deshpande. 1991. Epidemiology and clinico-pathology of hemoglobinuria in buffalo (Bubalus bubalis). Livestock Advisor, 16: 32-38.
Doumas, B.T., D.D. Bayse, R.J. Carter, T. Peters Jr and R. Schaffer. 1981. A candidate reference method for determination for total protein in serum. I. Development and validation. Clin. Chem., 27(10): 1642-1650.
Doumas, B.T., W.A. Watson and H.G. Biggs. 1971. Albumin standards and the measurement of serum albumin with bromocryzol green. Clin. Chem. Acta., 31(1): 87-96. DOI: 10.1016/0009-8981(71)90365-2
Durrani, A.Z., N. Kamal, A. Shakoori and R.M. Younus. 2010. Prevalence of post parturient haemoglobinuria in buffalo and therapeutic trials with toldimfos sodium and tea leaves in Pakistan. Turk. J. Vet. Anim. Sci., 34(1): 45-51.
Fayed, H., M. Ghanem, Y. Abdel-Raof and H.M. El-Attar. 2018. Hematobiochemical and urological alterations in buffaloes with post parturient haemoglobinuria. Benha Veterinary Medical Journal, 34(3): 287-294. DOI: 10.21608/BVMJ.2018.47846
Feldman, G.S., N.R. Perkins and N.C. Jain. 2000. Schalm’s Veterinary Heamatology, 4th ed. Lea and Fibiger, Philadelphia, USA.
Forrester, S.D. and K.J. Moreland. 1989. Hypophosphatemia. Causes and clinical consequences. J. Vet. Intern. Med., 3(3): 149-159. DOI: 10.1111/j.1939-1676.1989.tb03091.x
Goff, J.P. 2000. Pathophysiology of calcium and phosphorus disorders. Vet. Clin. N. Am., 16(2): 319-337. DOI: 10.1016/s0749-0720(15)30108-0
Grunberg, W. 2008. Phosphorous homostasis in dairy cattle: Some answers, more questions. In Proceedings of The 17th Annual Tri-State Dairy Nutrition Conference, Fort Wayne, Indiana, USA.
Grunberg, W. 2017. Metabolic disorders of phosphorous metabolism, hypophosphatemia. Veterinary Manual. Available on: www.msd.vet.manual.com
Kaneko, J.J., J.W. and M.L. 1997. Clinical Biochemistry of Domestic Animals, 5th ed. Academic Press San Diego, California, USA.
Konchel, J.P. 2006. Modern nutritional in Health and Disease, 10th ed. Baltimore, Lippincott Williams and Wikins, Philadelphia , USA.
Kumar, A., V. Thakur, P. Sandeep, S. Harpreet, R. Swati, G. Anita, R. Biswa and R.S. Bisla. 2019. Study on incidence, haemato biochemical changes and therapeutic management of post parturient haemoglobinuria in Murrah buffaloes. The Pharma Innovation Journal, 8(1): 147-150.
Kurek, L., K. Lutnicki and A. Barch. 2010. Various types of hypophosphatemia in dairy cows and the clinical implications depending on the intensity of the deficiency. B. Vet. I. Pulawy, 54(1): 35-41.
Latimer, K.S., E.A. Mahaffey, K.W. Prasse and S. Ducan. 2003. Veterinary Laboratory Medicine: Clinical Pathology, 4th ed. Iowa State Press, Iowa, USA.
Mahmut, O.K., H. Guzelbektes, I. Sen, A. Coskun and A.S. Ozturk. 2009. Post-parturient haemoglobinuria in three dairy cows. A case report. B. Vet. I. Pulawy, 53(3): 421-423.
Malik, H.U. and A. Samad. 1996. Pathogenesis of phosphorous deficiency haemoglobinuria in buffaloes. Influence of extracellular inorganic phosphorous on glucose metabolism in buffalo erythrocytes. Buffalo Journal, 12: 73-84.
Marik, P.E. and R. Bellomo. 2013. Stress hyperglycemia: An essential survival response. Crit. Care, 17(2): 305. DOI: 10.1186/cc12514
Muhammad, G., M. Saqib and M. Athar. 2001. A rational approach to diagnosis, treatment and control of parturient hemoglobinuria (red water) in buffaloes and cattle. Pak. Vet. J., 21(4): 214-219.
Navjot, S., K. Palneez, S. Harbir and Sh. Neelesh. 2017. Postpartum hemoglobinuria (PPH) in bovine. Theriogenology Insight, 7(1): 51-59. DOI: 10.5958/2277-3371.2017.00016.X
Purohit, G.N., G. Trilok, K. Amit, Sh. Atul, G. Mitesh and Sh. Chandra. 2018. Perspectives of parturient hemoglobinuria (pph) in buffaloes. International Journal of Development Research, 2: 23513-23520.
Rahmati, S., A. Aziz, M. Tawfeeq, J. Zabuli and S. Nazhat. 2021. Clinical features of post-parturient hemoglobinuria in dairy cattle and buffaloes. Open Journal of Veterinary Medicine, 11(4): 143-155. DOI: 10.4236/ojvm.2021.114010
Rana, J.P. and R.M. Bhardwaj. 1988. Relationship of intraerythrocytic ATP and membrane phospholipids to red cell shape in hemoglobinuric buffaloes. In The Proceeding 2nd World Buffalo Congress, New Delhi, India. p. 178-184.
Rock, R.C., W.G. Walker and C.D. Jennings. 1987. Nitrogen metabolites and renal function. In Fundamentals of Clinical Chemistry 3rd ed. WB Saunders, Philadelphia, USA.
Sarma, K., M. Saravanan, K. Pankaj, M. Kumar, R.K. Jadav and D.B. Monda. 2014. Influence on haemato-biochemical and oxidative indices of post parturient haemoglobinuric (PHU) buffalo. Buffalo Bull., 33(4): 343-348. Available on: https://ibic.lib.ku.ac.th/e-bulletin/IBBU201404013.pdf
Stockdale, C.R., T.E. Moyes and R. Dyson. 2005. Acute post-parturient haemoglobinuria in dairy cows and phosphorus status. Aust. Vet. J., 83(6): 362-266. DOI: 10.1111/j.1751-0813.2005.tb15635.x
Tamhane, A.C. and D.D. Dunlop. 2000. Statistics and Data Analysis from Elementary to Intermediate. Upper Saddle River, USA.
Tietz, N.W. 1990. Clinical Guide to Laboratory Tests, 2nd ed. WB Saunders Company, Philadelphia, USA. p. 444-447.
Tietz, N.W. 1995. Clinical Guide to laboratory Tests, 3rd ed. WB Saunders Company, Philadelphia, USA. p. 102.
Trinder, P. 1969. Enzymatic determination of glucose. Ann. Clin. Biochem., 6: 24-26.
Vassault, A., D. Grafmeyer, J. de Graeve, R. Cohen, A. Beaudonnet and J. Bienvenu. 1986. Protocol for the validation of methods. Ann. Biol. Clin.-Paris, 44: 686-745.
Wang, X.L., C.H. Gallager, T.J. Mcclure, V.E. Reeve and P.J. Canfield. 1985. Bovine post-parturient haemoglobinuria: Effect of inorganic phosphate on red cell metabolism. Res. Vet. Sci., 39(3): 333-339.
Zaghawa, A. Yousef and H, Nayel M. 2019. Clinical, epidemiological and clinic-pathological aspects of hypophosphatemia in buffaloes in Egypt. Bioscience Research, 16: 31-41.
Ziwei, Z., B.I. Mingyu and X.U. Shiwen. 2017. Effect of phosphorus deficiency on erythrocytic morphology and function in cows. Vet. Sci., 18(3): 333-340. DOI: 10.4142/jvs.2017.18.3.333
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2021-11-11
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El-Deen, N. N., Neamat-Allah, A., Rizk, L., & Fareed, R. S. (2021). Hematobiochemical alterations in clinical and subclinical hypophosphatemia in Egyptian buffaloes (Bubalus bubalis). Buffalo Bulletin, 40(4), 1–11. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/4663
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