Characterization and validation of point mutation in MBL1 gene and its relationship with mastitis in Murrah buffalo (Bubalus bubalis)

Authors

  • Kamaldeep Dhundwal Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
  • B. L. Pander Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
  • D. S. Dalal Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
  • Ankit Magotra Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
  • Dinesh Mittal Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
  • M. Singh College Central Laboratory, College of Veterinary Science, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
  • Anika Malik Department of Veterinary and Animal Husbandry Extension Education, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
  • Asha Garg Animal Genomic Lab, Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

Keywords:

Murrah buffalo, Murrah, Bubalus bubalis, Polymorphism, mastitis, Exon, mutation, Buffalo, Bovine Mannose-Binding Lectins, MBL1, gene, polymorphism, exon

Abstract

Mannose Binding Lectins (MBL) gene possibly contributes to bacterial infection resistance and was proposed as a molecular marker for milk production traits to control mastitis A total of 60 Murrah buffaloes were selected to characterize exon 2 of MBL1 gene to identify polymorphism and its association with mastitis. A 401 bp PCR fragment of MBL1 gene targeting g.2686T>C was amplified and digested with HaeIII restriction enzyme. Genotype analysis using PCR-RFLP revealed a monomorphic CC banding pattern. Sequencing was also carried out to explore other SNPs. The result indicates highly conserved DNA sequence in Murrah buffalo. This study provides preliminary information that the targeted region of MBL1 gene in water buffalo has no significant association with mastitis resistance which maybe a breed specific characteristic. Since present study has formulated the results based on a relatively small sample, further studies are required to validate these results in large samples.

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Author Biographies

B. L. Pander, Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

Professor, Department of Animal Genetics and Breeding, LUVAS. Hisar

D. S. Dalal, Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

Professor, Department of Animal Genetics and Breeding, LUVAS. Hisar

Ankit Magotra, Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

Asistant Professor, Department of Animal Genetics and Breeding, LUVAS. Hisar

Dinesh Mittal, Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

Assistant Professor, Department of  Veterinary Public Health and Epidemiology, LUVAS. Hisar

M. Singh, College Central Laboratory, College of Veterinary Science, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

Scientist, College Central Laboratory, College of Veterinary science, LUVAS

Anika Malik, Department of Veterinary and Animal Husbandry Extension Education, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

Assistant Professor, Department of Veterinary and Animal Husbandry Extension Education, LUVAS, Hisar

Asha Garg, Animal Genomic Lab, Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

Research Worker, Animal Genomic Lab, Department of AGB, LUVAS. Hisar

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Published

2019-09-30

How to Cite

Dhundwal, K., Pander, B. L., Dalal, D. S., Magotra, A., Mittal, D., Singh, M., … Garg, A. (2019). Characterization and validation of point mutation in MBL1 gene and its relationship with mastitis in Murrah buffalo (Bubalus bubalis). Buffalo Bulletin, 38(3), 451–457. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/524

Issue

Vol. 38 No. 3 (2019): July-September

Section

Original Article

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