Genetic profiling of K232A mutation of DGAT1 gene in Murrah buffalo
DOI:
https://doi.org/10.56825/bufbu.2024.4343561Keywords:
Bubalus bubalis, buffaloes, DGAT1, mutation, candidate gene, MurrahAbstract
The aim of this study was to characterize and validate the candidate K232A in in Murrah buffalo’s Diacylglycerol O-acyltransferase1 (DGAT1) gene. The DGAT1 gene has been identified as a possible candidate gene for milk fat. EaeI restriction enzyme was used to digest a 413 bp PCR product that covered the DGAT1 gene’s exon 7, intron 8, exon 9, and partial exon 9 regions. This allowed us to screen for the reported mutation. Within our resource population, a monomorphic banding pattern with genotype KK was discovered. Additionally, sequencing was done to evaluate and validate the screening mutation in a specific region’s nucleotide sequence. The outcome suggests that the Murrah buffalo has a substantially conserved sequence.
Downloads
Metrics
References
Berry, D.P., D. Howard, P. O’Boyle, S. Waters, J.F. Kearney and M. Mc Cabe. 2010. Associations between the K232A polymorphism in the diacylglycerol-O-transferase 1 (DGAT1) gene and performance in Irish Holstein-Friesian dairy cattle. Irish J. Agr. Food Res., 49(1): 1-9. DOI: 10.2307/20788561
Borghese, A. 2005. Buffalo Production and Research, Reu Technicalseries 67. Food and Agriculture Organization of the United Nations, Rome, Italy.
Cases, S., S.J. Smith, Y.W. Zheng, H.M. Myers, S.R. Lear, E. Sande, S. Novak, C. Collins, C.B. Welch, A.J. Lusis and S.K. Erickson. 1998. Identification of a gene encoding an acyl CoA: Diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. In The Proceedings of the National Academy of Sciences, 95(22): 13018-13023. DOI: 10.1073/pnas.95.22.13018
Ganguly, I., S. Kumar, G.K. Gaur, U. Singh, A. Kumar, S. Kumar and S. Mann. 2013. DGAT1 polymorphism K232A in Sahiwal (Indian Zebu) and frieswal (Holstein friesian X Sahiwal crossbred) cattle. Indian J. Anim. Res., 47(4): 360-363.
Gautier, M., A. Capitan, S. Fritz, A Eggen, D. Boichard and T. Drue. 2007. Characterization of the DGAT1 K232A and variable number of tandam repeat polymorphisms in French dairy cattle. J. Dairy Sci., 90(6): 2980-2988. DOI: 10.3168/jds.2006-707
Grisart, B., W. Coppieters, F. Farnir, L. Karim, C. Ford, P. Berzi, N. Cambisano, M. Mni, S. Reid, P. Simon and R. Spelman. 2002. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Res., 12(2): 222-231. DOI: 10.1101/gr.224202
Grisart, B., F. Farnir, L. Karim, N. Cambisano, J.J. Kim, A. Kvasz, M. Mni, P. Simon, J.M. Frere, W. Coppieters and M. Georges. 2004. Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition. In The Proceedings of the National Academy of Sciences of the United States of America, 101(8): 2398-2403. DOI: 10.1073/pnas.0308518100
Gothwal, A., A. Magotra, Y.C. Bangar, B.S. Malik, A.S. Yadav and A.R. Garg. 2022. Candidate K232A mutation of DGAT1 gene associated with production and reproduction traits in Indian Dairy cattle. Anim Biotechnol., 34(7): 2608-2616. DOI: 10.1080/10495398.2022.2109041
Heydarian, D., S.R. Miraei-Ashtiani and M. Sadeghi. 2014. Study on DGAT1-exon8 polymorphism in Iranian buffalo. International Journal of Advanced Biological and Biomedical Research, 2(7): 2276-2282.
Jensen, R.G., A.M. Ferris and C.J. Lammi-Keefe. 1991. The composition of milk fat. J. Dairy Sci., 74(9): 3228-3243. DOI: 10.3168/jds.S0022-0302(91)78509-3
Kaupe, B., A. Winter, R. Fries and G. Erhardt. 2004. DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds. J. Dairy Res., 71(2): 182-187. DOI: 10.1017/s0022029904000032
Komisarek, J., K. Waskowicz, A. Michalak and Z. Dorynek. 2004. Effects of DGAT1 variants on milk production traits in Jersey cattle. Anim. Sci. Pap. Rep., 22(3): 307-313.
Kühn, C., G. Thaller, A. Winter, O.R.P. Bininda-Emonds, B. Kaupe, G. Erhardt, J. Bennewitz, M. Schwerin and R. Fries. 2004. Evidence for multiple alleles at the DGAT1 locus better explains a quantitative trait locus with major effect on milk fat content in cattle. Genetics, 167(4): 1873-1881. DOI: 10.1534/genetics.103.022749
Lacorte, G.A., M.A. Machado, M.L. Martinez, A.L. Campos, R.P. Maciel, R.S. Verneque, R.L. Teodoro, M.G.C.D. Peixoto, M.R.S. Carvalho and C. Fonseca. 2006. DGAT1 K323A polymorphism in Brazilian cattle breeds, Genet. Mol. Res., 5(3): 475-482.
Mishra, B., M.S. Tantia, S.T.B. Kumar and R.K. Vijh. 2007. Characterization of the DGAT1 gene in the Indian buffalo (Bubalus bubalis). Genet. Mol. Biol., 30(4): 1097-1100. DOI: 10.1590/S1415-47572007000600012
Pareek, C.S., U. Czarnik, T. Zabolewicz, R.S. Pareek and K. Walawski. 2005. DGAT1 K232A quantitative trait nucleotide polymorphism in Polish Black-and-White cattle. J. Appl. Genet., 46(1): 85-87.
Raut, A.A., A. Kumar, S.N. Kala, V. Chhokar and N. Rana. 2012. Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP. Genet. Mol. Biol., 35(3): 610-613. DOI: 10.1590/S1415-47572012005000043
Rosati, A. and L.D. Van Vleck. 2002. Estimation of genetic parameters for milk, fat, protein and mozzarella cheese production for the Italian river buffalo Bubalus bubalis population. Livest. Prod. Sci., 74(2): 185-190. DOI: 10.1016/S0301-6226(01)00293-7
Sambrook, J. and D.W. Russell. 2001. Molecular cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, New York, USA.
Sara Abu, S.M., A.S. Ahmed, M.K.A. Ahmed, M. Reissmann, G.A. Brockmann and S.A. Rahmatalla. 2015. Variants of the Diacylglycerol acyltransferase 1 (Dgatl) gene in Sudanese dairy cattle (Kenana and Butana). Journal of Molecular Genetics, 7(1-4): 5-9. DOI: 10.3923/jmolgene.2015.5.9
Spelman, R.J., C.A. Ford, P. McElhinney, G.C. Gregory and R.G. Snell. 2002. Characterization of the DGAT1 gene in the New Zealand dairy population, J. Dairy Sci., 85: 3514-3517. DOI: 10.3168/jds.S0022-0302(02)74440-8
Strzalkowska, N., E. Siadkowska, K. Sloniewski, J. Krzyzewski and L. Zwierzchowski. 2005. Effect of the DGAT1 gene polymorphism on milk production traits in Black-and-White (Friesian) cows. Animal Science Papers and Reports, 23(3): 189-197.
Tantia, M.S., R.K. Vijh, B.P. Mishra, B. Mishra, S.B. Kumar and M. Sodhi. 2006. DGAT1 and ABCG2 polymorphism in Indian cattle (Bos indicus) and buffalo (Bubalus bubalis) breeds. BMC Vet. Res., 2(1): 32. DOI: 10.1186/1746-6148-2-32
Thaller, G., W. Kramer, A. Winter, B. Kaupe, G. Erhardt and R. Fries. 2003. Effects of DGAT1 variants on milk production traits in German cattle breeds. J. Anim. Sci., 81(8): 1911-1918. DOI: 10.2527/2003.8181911x
Tonhati, H., F.B. Vasconcellors and L.G. Albuquerque. 2000. Genetic aspects of productive and reproductive traits in a Murrah buffalo herd in São Paulo, Brazil. J. Anim. Breed Genet., 117(5): 331-336. DOI: 10.1046/j.1439-0388.2000.00249.x
Venkatachalapathy, R.T., A. Sharma and K. Radha. 2014. Polymorphism at DGAT1 locus in Indian buffalo, zebu and Bos indicus × Bos taurus cattle breeds. Veterinary Science Research Journal, 5(1/2): 13-17. DOI: 10.15740/HAS/VSRJ/5.1and2/13-17
Weller, J.I., M. Golik, E. Seroussi, E. Ezra and M. Ron. 2003. Population-wide analysis of a QTL affecting milk-fat production in the Israeli Holstein population. J. Dairy Sci., 86(6): 2219-2227. DOI: 10.3168/jds.S0022-0302(03)73812-0
Winter, A., W. Kramer, F.A. Werner, S. Kollers, S. Kata, G. Durstewitz, J. Buitkamp, J.E. Womack, G. Thaller and R. Fries. 2002. Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. P. Natl. Acad. Sci. USA., 99(14): 9300-9305. DOI: 10.1073/pnas.142293799
Yuan, J., J. Zhou, X. Deng, X. Hu and N. Li. 2007. Molecular cloning and single nucleotide polymorphism detection of buffalo DGAT1 gene. Biochem Genet., 45: 611-621. DOI: 10.1007/s10528-007-910FSiBundleContentBlockBundleEntityTranslatableBlockTranslatableFilesElement/filePath/187/strona307-314.pdf
Downloads
Published
How to Cite
Issue
Section
.png){kind=logo}
