IDENTIFICATION OF ANATOLIAN BUFFALOES BREED IN THE PROVINCE OF TOKAT BASED ON COI (MITOCHONDRIAL CYTOCHROME C OXIDASE SUBUNIT I) GENE REGION
DOI:
https://doi.org/10.56825/bufbu.2025.4425851Keywords:
Bubalus bubalis, buffaloes, Anatolian buffalo , DNA barcoding , phylogenetic tree , COI gene , molecular identificationAbstract
This study aimed to barcode the COI gene region for molecular identification and taxonomic classification of Anatolian buffaloes breed in the province of Tokat. In the study, blood samples were taken from 20 randomly selected Anatolian buffaloes breed in the Central, Turhal, and Pazar districts of Tokat. Sequence data of 709-base pair long COI gene region in Anatolian buffaloes were obtained. The sequences of COI gene region in buffalo species were analyzed in the MEGA program and a phylogenetic tree was created.
In light of the data, the intra-species genetic difference of the population in Tokat was calculated as 0.002 and the inter-species genetic distance was statistically insignificant. In addition, the inter-species distance was determined by a phylogenetic tree. As a result of the phylogenetic tree, it was confirmed that Anatolian buffaloes breed in Tokat belong to Bubalus Bubalis species. For five of the studied buffalo samples formed a subgroup. The other samples were divided into different branches.
In conclusion, it is thought that the study findings will significantly contribute to the literature, that DNA barcoding techniques can be used in determination the genetic characterization of Anatolian buffaloes, and that it will shed light on relevant studies to be carried out.
Downloads
Metrics
References
Atasever, S. and H. Erdem. 2008. Manda yetiştiriciliği ve Türkiye’deki geleceği. Anadolu Tarım Bilimleri Dergisi 23(1): 59-64. (In Turkish).
Cooper, J.K., G. Sykes, S. King, K. Cottrill, N.V. Ivanova, R. Hanner and P. Ikonomi. 2007. Species identification in cell culture: A two-pronged molecular approach. In Vitro Cell Dev. An., 43(10): 344-51. DOI: 10.1007/s11626-007-9060-2
Şahin, A., Y. Aksoy, A. Yıldırım, Z. Ulutaş. 2023. Determination of some factors affecting the twelfth months live weight of Anatolian buffaloes using the CHAID algorithm. Journal of Kırşehir Ahi Evran University, 3(2): 270-277.
Hassan, A.A.M., E.A. Balabel, H.A.S. Oraby and S.A. Darwish. 2018. Buffalo species identification and delineation using genetic barcoding markers. Journal of Genetic Engineering and Biotechnology, 16(2): 499-505. DOI: 10.1016/j.jgeb.2018.07.006
Hebert, P.D.N., A. Cywinska, S.L. Ball and J.R. deWaard. 2003a. Biological identifications through DNA barcodes. P. Roy. Soc. B.-Biol. Sci., 270(1512): 313-321. DOI: 10.1098/rspb.2002.2218
Hebert, P.D.N., S. Ratnasingham and J.R. Dewaard. 2003b. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. P. Roy. Soc. B.-Biol. Sci., 270(Suppl 1): S96-S99.
Hebert, P.D.N. and T.R. Gregory. 2005. The promise of DNA barcoding for taxonomy. Syst. Biol., 54(5): 852-859. DOI: 10.1080/10635150500354886
Haider, N., I. Nabuls and B. Al-Safadi. 2012. Identification of meat species by PCR-RFLP of the mitochondrial COI gene. Meat Sci., 90(2): 490-493. DOI: 10.1016/j.meatsci.2011.09.013
Ibtisham, F., L. Zhang, M. Xiao, L. An, M.B. Ramzan, A. Nawab, Y. Zhao, G. Li and Y.M. Xu. 2017. Genomic selection and its application in animal breeding. Thai J. Vet. Med., 47(3): 301-310. DOI: 10.56808/2985-1130.2838
Kress, W.J., K.J. Wurdack, E.A. Zimmer, L.A. Weigt and D.H. Janzen. 2005. Use of DNA barcodes to identify flowering plants. P. Natl. Acad. Sci. USA, 102(23): 8369-8374. DOI: 10.1073/pnas.0503123102
Özcan, S., G. Ekrem and M. Babaoğlu. 2004. Bitki Biyoteknolojisi, Genetik Mühendisliği Uygulamaları II, S.Ü. Basımevi Konya, Turkiye. 456p.
Priyono, D.S., D.D. Solıhın, A. Farajallah, D.I.D. Arını. 2018. Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode. Biodiversitas, 19(6): 1985-1992. DOI: 10.13057/biodiv/d190602
Tamburrano, A., B. Tavazzi, C.A.M. Callà, A.M. Amorini, G. Lazzarino, S. Vincenti, T. Zottola, T.C. Campagna, U, Moscato and P. Laurenti. 2019. Biochemical and nutritional characteristics of buffalo meat and potential implications on human health for a personalized nutrition. Italian Journal of Food Safety, 8(3): 8317. DOI: 10.4081/ijfs.2019.8317
Saputra, F. and C. Sumantri. 2014. Genetic variation of mtDNA cytochrome oxidase subunit I (COI) in local Swamp buffaloes in Indonesia. Media Peternakan, 36(3): 165. DOI: 10.5398/medpet.2013.36.3.165
Spychaj, A., M. Szalata, R. Słomski and E. Pospiech. 2016. Identification of bovine, pig and duck meat species in mixtures and in meat products on the basis of the mtDNA cytochrome oxidase subunit I (COI) gene sequence. Pol. J. Food Nutr. Sci., 66(1): 31-36. DOI: 10.1515/pjfns-2015-0051
Walsh, B. 2000. Minireview: Quantitative genetics in the age of genomics. Theor. Popul. Biol., 59(3): 175-184. DOI: 10.1006/tpbi.2001.1512
Waugh, J. 2007. DNA barcoding in animal species: progress, potential and pitfalls. BioEssays, 29(2): 188-197. DOI: 10.1002/bies.20529
