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Artificial insemination with cryopreserved semen is the most viable biotechnology for faster and increased genetic improvement in many species allowing for improved herd performance and productivity. Pakistan has 34.6 million buffalo which have major share in total milk produced in the country. Rapid increase in human population and demand for animal products motivated the researchers to increase per animal milk production using this biotechnology. In buffalo, natural breeding practice is common in the country compared to artificial insemination, low fertility rate with cryopreserved semen is main hindrance in its propagation. It is need of the hour to disseminate the knowledge of various factors and components contributing in buffalo semen cryopreservation to improve milk and fertility rate of this breed.