Morphological studies of cryopreserved Toda buffalo spermatozoa by CASA

Authors

  • R. Anilkumar Sheep Breeding Research Station, Tamilnadu Veterinary and Animal Sciences University, Sandynallah, Ooty, The Nilgiris, Tamilnadu, India
  • M.N. Sundararaman Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai, India,
  • D.V. Patel Sabarmati Ashram Gaushala, Bidaj Farm, PO- Lali, Dist, Kheda, Gujarat, India
  • M. Iyue Sheep Breeding Research Station, Tamilnadu Veterinary and Animal Sciences University, Sandynallah, Ooty, The Nilgiris, Tamilnadu, India
  • R. Kasiraj Sabarmati Ashram Gaushala, Bidaj Farm, PO- Lali, Dist, Kheda, Gujarat, India

Keywords:

Buffaloes, Bubalus bubalis, Pasteurella multocida, Multilocus sequence typing, Sequence type, Allelic profile, Gujarat, India

Abstract

Toda buffaloes are the endangered species of the Nilgiri Hills of South India. Successful attempts have been made in cryopreservation of semen of Toda bulls. Thawed semen samples from Toda buffalo bulls were used to analyze the spermatozoal morphometry by Computer assisted semen analyzer (CASA). Head length (major axis), width (minor axis), area, perimeter, elongation and tail length were the morphometrical characteristics measured. The mean (±SE) length, width, area, perimeter, elongation percentage and tail length were 7.38±0.02 µm, 4.68±0.02 µm, 28.53±0.17 µm2 , 20.32±0.19 µm, 62.95±0.22% and 52.93±0.41 µm respectively. Overall spermatozoal abnormality was 30.75% and coiled tail was the most common (50.49%) abnormality. The spermatozoal morphometry reported in this study may form a preliminary data in Toda buffalo semen analysis and helps for comparison with other breeds and can complement sperm motility assessment.

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Published

2017-10-11

How to Cite

Anilkumar, R., Sundararaman, M., Patel, D., Iyue, M., & Kasiraj, R. (2017). Morphological studies of cryopreserved Toda buffalo spermatozoa by CASA. Buffalo Bulletin, 36(2), 447–453. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/724

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Original Article