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The aim of the present study was to compare plasma mineral status of anoestrus and normally cyclic cattle and buffaloes of central zone of Punjab and to evaluate the use of area specific mineral mixture as a treatment of anoestrus in dairy animals. Thirty two non-cyclic animals (16 cattle, 16 buffaloes) and 32 cyclic animals (16 cattle, 16 buffaloes) in the 2nd t o 3rd lactation were selected for the study on basis of rectal examination and plasma progesterone levels at 0, 7 and 14 days by Radio Immuno Assay (RIA) technique. Blood samples from these animals were analyzed for various minerals viz., Ca, Mg, P, Cu, Zn, Fe and Mn. Anoestrus dairy animals had significantly lower levels of calcium, phosphorus and copper as compared to cyclic animals. For therapeutic trial, anoestrus animals (10 cattle and 10 buffaloes) kept as treatment group were fed area specific mineral mixture (50 gm/animal/ day) for 2 months. In total, 17 animals out of 20 (85%) regained cyclicity i.e. had at least one value of plasma progesterone level above 0.5 ng/ml out of 3 weekly samples collected. Average time taken for animals to come into heat after area specific mineral mixture supplementation was found to be 53.4±2.7 and 48.9±2.1 days in buffaloes and cattle, respectively.