Ecological phytochemistry of medicinal Trema orientalis (L.) Blume (Cannabaceae) in Phu Meang-Phu Thong forest group of Thailand

Authors

  • Apsonsawan Jaiboon Biotechnology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province
  • Keerati Tanruean Biotechnology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province / Biology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province
  • Pisit Poolprasert Biology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province
  • Pornpat Sam-ang Chemistry major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province
  • Manop Poopath The Forest Herbarium, Forest Botany division, Department of National Parks Wildlife and Plant conservation, Bangkok
  • Kusuma Phumkhonsarn Protected Area Regional Office 11, Department of National Parks Wildlife and Plant conservation, Phitsanulok province
  • Wichai Santimaleeworagun Department of Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom province
  • Tiwtawat Napiroon Biotechnology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province / Biology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province

Keywords:

Biotechnology, Chemodiversity, Cannabaceae, Forest ecology

Abstract

Trema orientalis (L.) Blume in family Cannabaceae is wildly distributed in the tropical regions. In Thailand, these plants were found in various habitats, all plant parts were used as traditional medicine in the treatment of infection. The purpose of our work was to compare the chemical profiles among similar plant part extracts from five different ecological habitats includes hill evergreen forest, mixed deciduous forest, coniferous forest, edge and disturbed forest and agricultural areas at the Phu Meang-Phu Thong forest complex using TLC and HPLC chromatographic techniques. The lipophilic extracts of all plant parts were analyzed for chemical characters with TLC, mobile phase (hexane : ethyl acetate, 8:2 v/v) and UV illuminated detection (wavelength 254 and 365 nm). HPLC, mobile phase consisted in a solvent system, 60% v/v methanol in 40% v/v aqueous buffer, wavelength 230 nm. TLC analysis showed single band separation and Rf values measurement at the positive results of terpenoids, phenolic compounds, coumarins and sterols. For quantitative HPLC, each plant part extract (10 mg/mL) showed signals appearing of UV detection nearly or at the same position, as same as their UV absorbance values in each dominant compound. The retention time period of dominant peak was repeatability in all replication with retention time in average of leaf extracts at 2.73 and 27.24 min, stem bark extracts at 2.76, 23.18, 25.89 and 29.57 min and the inflorescence and infructescence extracts at 2.78, 26.73, 27.60 and 29.40 min respectively. Moreover, our results also showed the chemical pattern compounds that tend to accumulate highly in each plants part of disturbed and agricultural habitat which are useful to guide the bioactive compound isolation from T. orientalis. For habitat relationships, the chemical profiles in each plants part extracts from different ecological habitats were classified in 2 groups which consisted of leaf extracts and stem bark together with inflorescence and infructescence extracts which related to compounds appearing and Rf value factors analyzed by PCA analysis.

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Author Biographies

Apsonsawan Jaiboon, Biotechnology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province



Keerati Tanruean, Biotechnology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province / Biology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province




Pisit Poolprasert, Biology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province



Pornpat Sam-ang, Chemistry major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province





Manop Poopath, The Forest Herbarium, Forest Botany division, Department of National Parks Wildlife and Plant conservation, Bangkok




Kusuma Phumkhonsarn, Protected Area Regional Office 11, Department of National Parks Wildlife and Plant conservation, Phitsanulok province




Wichai Santimaleeworagun, Department of Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom province



Tiwtawat Napiroon, Biotechnology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province / Biology major, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok province




The comparison of retention times (min) and intensity of absorbance (mAU) in plant parts lipophilic extracts (10 mg/mL concentration) from different habitats.

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Published

2020-07-22

Issue

Vol. 4 No. 2 (2020): July - December

Section

Original Article