Evaluation of fresh semen quality and predicting the number of frozen semen doses in Jaffrabadi buffalo bull

Abstract

Scientific information on frozen semen characteristics of Jaffrabadi buffaloes (Bubalis bubalis) is scanty and centre to this research is to evaluate frozen semen characteristics in Jaffrabadi buffalo bulls. The study included six buffalo bulls with average age 95.83±5.47 months and average body weight of 834.16±47.63 kg maintained at Cattle Breeding Farm, Junagadh Agricultural University, Junagadh. A total of 206 ejaculates were collected during the period of study. Ejaculates collected from the six Jaffrabadi buffalo bulls were clean, dense to very dense (D = 67.2%, DD = 32.8%) and milky white (72.5%) to creamy (27.5%) in colour. During this observatory study average (Mean±SE) semen parameter like, ejaculatory volume, mass activity, sperm concentration, initial progressive sperm motility and total sperm number per ejaculate were 5.11±0.17 ml, +3.43±0.04, 838.30±25.74×106 /ml, 79.41±0.60% and 4053.99±150.56×106 spermatozoa, respectively. Average dilution rate in 0.5 ml medium straw (40 million sperm/straw) was found to be ideal at 10.48±0.32 ml. In a year expected number of ejaculates that could be frozen from the 6 bulls was 34.34±6.43 and correspondingly, the expected number of frozen doses produced from bulls could be 3546.46±540.30 numbers.