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The present study was designed to investigate the incidence of retention of fetal membranes (RFM) on 605 buffaloes at 2nd to 5th calving with normal calving, premature birth and abortion. The normally calved buffaloes with retention of fetal membranes (after 12 h) were randomly classified in treatment (n = 6) and control (n = 6) groups. The buffaloes in treatment group were administered Dinoprost (PGF2α analogue, 25 mg); Meloxicam (anti-inflammatory drug 0.5 mg/kg body weight,); Oxytetracycline (Broadspectrum antibiotics, 5 mg/kg body weight) intramuscularly and intravenous calcium therapy (Calcium gluconate, 1.86% w/v; Magnesium hypophosphite, 5% w/v; Dextrose anhydrous, 20% w/v; Chlorocresol 0.1% w/v, 450 ml) after 12 after calving. The incidence of retention of fetal membranes in buffaloes was 9.98 %, 34.61% and 46.87% in normal calving, premature birth and abortion, respectively. The overall incidence of RFM was 14.04%. There was significant rise in the level of calcium (P<0.01), phosphorus (P<0.05) and magnesium (P<0.05) on day 30 and day 45 in treatment than control group. However, there was no significant difference observed in level of glucose, NEFA and progesterone between treatment and control group on day 0, 30 and 45. RFM is considered as one of the major problem with higher incidence in premature birth and abortions by ultimately affecting the reproductive efficiency in buffaloes.