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This study compared the effect of kisspeptin-10 or GnRH administration on LH release in swamp buffalo during the luteal phase of the estrous cycle. Six animals were treated with a single intravenous injection of 1,000 pmol/kg b.w. of kisspeptin-10 or a single intramuscular injection of 10 µg/cow of GnRH agonist buserelin. For plasma LH analysis blood samples were collected every 15 minutes, 1 h before and 6 h after kisspeptin-10 and GnRH administration. An increase in LH plasma concentrations was observed after GnRH administration but not after kisspeptin-10 administration. The results of this study indicate that during the luteal phase of the estrous cycle, administration of GnRH, but not kisspeptin-10, stimulate LH secretion.