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The present study was conducted on 24 buffaloes having leukoderma. Three treatment protocols were evaluated. The cases were diagnosed on the basis of history and clinical signs and later confirmed by micro mineral status. Hematology and serum micro minerals status were evaluated before and after the treatment. Animals in Group 1, treated with injection copper glycinate of 1 ml/ 100 kg bwt S/C at interval of 10 days, showed 66.67% recovery in 60 days. Recovery in second group administered multi mineral injection 10 ml S/C at weekly interval was 83.33% in 60 days. In group third mineral mixture powder was administered 50 gram orally daily till 60 days with 16.67% recovery. Recovery was adjudged by the disappearance of the clinical signs. After end of treatment the clinical observations and analysis of all the findings of hematological and serum mineral values revealed that Group 2 had best therapeutic response.